Abstract
The clinical and histopathological manifestations of the infection of immunosuppressed (cyclophosphamide-treated) and immunocompetent (control) adult mice with the CVS ts 2 strain of fixed rabies virus were correlated with the kinetics of virus multiplication in the central nervous system and with the development of serum antibody. In immunocompetent mice severe paralytic disease causing 80% mortality was accompanied by marked inflammation and degeneration of the central nervous system parenchymatous tissue. Antirabies antibody was detected in all immunocompetent mice severely paralyzed from postinoculation day 6 on; virus was rarely isolated. In contrast, immunosuppressed mice developed encephalitic symptoms with only minor paralysis; the infection was 100% fatal. Histopathological changes in immunosuppressed mice were confined to degeneration and necrosis of individual neurons and mild microglial reaction; virus was isolated from all of these mice. No significant level of antibody was detected. Similar manifestations were seen after infection of immunodeficient (athymic) mice except that the athymic mice developed levels of antibody similar to those of control mice on day 6; antibodies in athymic mice were predominantly of immunoglobulin class M.
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- Clark H. F., Cohen M. M., Lunger P. D. Comparative characterization of a C-type virus-producing cell line (VSW) and a virus-free cell line (VH2) from Vipera russelli. J Natl Cancer Inst. 1973 Aug;51(2):645–657. [PubMed] [Google Scholar]
- Clark H. F., Ohtani S. Temperature-sensitive mutants of rabies virus in mice: a mutant (ts2) revertant mixture selectively pathogenic by the peripheral route of inoculation. Infect Immun. 1976 May;13(5):1418–1425. doi: 10.1128/iai.13.5.1418-1425.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cohen S. L., Gardner S., Lanyi C., McDonald J. R., Rée H., Southorn P. A., Woodruff A. W. A case of rabies in man: some problems in diagnosis and management. Br Med J. 1976 May 1;1(6017):1041–1042. doi: 10.1136/bmj.1.6017.1041. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gerhard W., Braciale T. J., Klinman N. R. The analysis of the monoclonal immune response to influenza virus. I. Production of monoclonal anti-viral antibodies in vitro. Eur J Immunol. 1975 Oct;5(10):720–725. doi: 10.1002/eji.1830051013. [DOI] [PubMed] [Google Scholar]
- Maton P. N., Pollard J. D., Davis J. N. Human rabies encephalomyelitis. Br Med J. 1976 May 1;1(6017):1038–1040. doi: 10.1136/bmj.1.6017.1038. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sedwick W. D., Wiktor T. J. Reproducible plaquing system for rabies, lymphocytic choriomeningitis,k and other ribonucleic acid viruses in BHK-21-13S agarose suspensions. J Virol. 1967 Dec;1(6):1224–1226. doi: 10.1128/jvi.1.6.1224-1226.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Segal G. P., Klinman N. R. Defining the heterogeneity of anti-tumor antibody responses. J Immunol. 1976 Jun;116(6):1539–1546. [PubMed] [Google Scholar]
- Sikes R. K., Cleary W. F., Koprowski H., Wiktor T. J., Kaplan M. M. Effective protection of monkeys against death from street virus by post-exposure administration of tissue-culture rabies vaccine. Bull World Health Organ. 1971;45(1):1–11. [PMC free article] [PubMed] [Google Scholar]
- Wiktor T. J., Doherty P. C., Koprowski H. In vitro evidence of cell-mediated immunity after exposure of mice to both live and inactivated rabies virus. Proc Natl Acad Sci U S A. 1977 Jan;74(1):334–338. doi: 10.1073/pnas.74.1.334. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wiktor T. J., Kuwert E., Koprowski H. Immune lysis of rabies virus-infected cells. J Immunol. 1968 Dec;101(6):1271–1282. [PubMed] [Google Scholar]