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. 2014 Oct 23;16(10):771–788. doi: 10.1016/j.neo.2014.08.013

Figure 1.

Figure 1

Normal neutrophils are the major producer of MMP-9. (A) Unfractionated PB from WT mice was immunostained for Gr-1 (green) and MMP-9 (red). Cell nuclei were highlighted with DAPI (blue). Bar, 5 μm. (B–D) Neutrophils were isolated from PB or BM of WT or Mmp9-KO mice, whereas macrophages (Mϕs) were generated from adherent BM mononuclear cells. Bars, 10 μm (B) HEMA staining. (C) Immunostaining (green) for Ly6G or F4/80. (D) Immunostaining (green) for MMP-9. (E–H) Zymographic analysis of MMP-9. Recombinant murine proMMP-9 (Rec. MMP-9) provided standards for quantification of MMP-9. Arrows point to the 105-kDa monomers, ~ 130-kDa heterodimers, and ~ 270-kDa multimers of proMMP-9. (E) Cell lysates (CL) of BMD-Mϕs and neutrophils isolated from PB or BM of WT or Mmp9-KO mice. (F) CL and releasates (RL) from WT PB neutrophils and CL and CM from WT PBD-Mϕs. (G) RL from PB and BM neutrophils isolated from WT and Mmp9-KO mice. (H) CM from WT BMD-Mϕs.