Figure 8.

Re-enforced polarization toward M2 phenotype increases proMMP-9 production and angiogenesis-inducing capacity of LLC TAMs. (A) Forty-eight-hour SF CM from WT LLC TAMs, incubated with IL-4 to “re-enforce” the M2 phenotype or left not treated (NT), was analyzed for proMMP-9 and TIMP-1. (B) Freshly isolated TAMs were compared with TAMs treated in vitro with IL-4 or left not treated (NT) for angiogenesis-inducing capacity (3 × 10⁴ cells per onplant). Pooled data from two independent experiments, each employing from four to six embryos grafted with four to six onplants per variant, are presented. Data are means ± SEM. *P < .05, **P < .005, ***P < .0001.