Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1977 Nov;18(2):348–351. doi: 10.1128/iai.18.2.348-351.1977

Escherichia coli heat-labile enterotoxin: comparison of antitoxin assays and serum antitoxin levels.

I K Wachsmuth, J G Wells, R W Ryder
PMCID: PMC421238  PMID: 336543

Abstract

The mouse adrenal tumor cells (Y-1 strain) and the Chinese hamster ovary cells, two routinely used tissue culture assays for Escherichia coli heat-labile enterotoxin (LT), were used to detect serum antitoxin responses in culture-positive patients from several well-defined sources. There was no correlation between a significant antitoxin response and isolation of LT-producing E. coli in two "domestic" diarrheal outbreaks. Serum samples from a third group of individuals in a rural cholera-endemic area consistently demonstrated significant rises in neutralizing antibody to LT.

Full text

PDF
348

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Donta S. T. Editorial: Gastroenterologists on the move: the nature of travelers' diarrhea. N Engl J Med. 1976 Jun 10;294(24):1340–1341. doi: 10.1056/NEJM197606102942409. [DOI] [PubMed] [Google Scholar]
  2. Donta S. T., Moon H. W., Whipp S. C. Detection of heat-labile Escherichia coli enterotoxin with the use of adrenal cells in tissue culture. Science. 1974 Jan 25;183(4122):334–336. doi: 10.1126/science.183.4122.334. [DOI] [PubMed] [Google Scholar]
  3. Donta S. T., Sack D. A., Wallace R. B., Dupont H. L., Sack R. B. Tissue-culture assay of antibodies to heat-liable Escherichia coli enterotoxins. N Engl J Med. 1974 Jul 18;291(3):117–121. doi: 10.1056/NEJM197407182910302. [DOI] [PubMed] [Google Scholar]
  4. Evans D. G., Evans D. J., Jr, Gorbach S. L. Identification of enterotoxigenic Escherichia coli and serum antitoxin activity by the vascular permeability factor assay. Infect Immun. 1973 Nov;8(5):731–735. doi: 10.1128/iai.8.5.731-735.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Guerrant R. L., Brunton L. L. Characterization of the Chinese hamster ovary cell assay for the enterotoxins of Vibrio cholerae and Escherichia coli and for specific antisera, and toxoid. J Infect Dis. 1977 May;135(5):720–728. doi: 10.1093/infdis/135.5.720. [DOI] [PubMed] [Google Scholar]
  6. Guerrant R. L., Brunton L. L., Schnaitman T. C., Rebhun L. I., Gilman A. G. Cyclic adenosine monophosphate and alteration of Chinese hamster ovary cell morphology: a rapid, sensitive in vitro assay for the enterotoxins of Vibrio cholerae and Escherichia coli. Infect Immun. 1974 Aug;10(2):320–327. doi: 10.1128/iai.10.2.320-327.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gyles C. L. Immunological study of the heat-labile enterotoxins of Escherichia coli and Vibrio cholerae. Infect Immun. 1974 Mar;9(3):564–570. doi: 10.1128/iai.9.3.564-570.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Merson M. H., Morris G. K., Sack D. A., Wells J. G., Feeley J. C., Sack R. B., Creech W. B., Kapikian A. Z., Gangarosa E. J. Travelers' diarrhea in Mexico. A prospective study of physicians and family members attending a congress. N Engl J Med. 1976 Jun 10;294(24):1299–1305. doi: 10.1056/NEJM197606102942401. [DOI] [PubMed] [Google Scholar]
  9. Morris G. K., Merson M. H., Sack D. A., Wells J. G., Martin W. T., Dewitt W. E., Feeley J. C., Sack R. B., Bessudo D. M. Laboratory investigation of diarrhea in travelers to Mexico: evaluation of methods for detecting enterotoxigenic Echerichia coli. J Clin Microbiol. 1976 May;3(5):486–495. doi: 10.1128/jcm.3.5.486-495.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Portnoy B. L., Du Pont H. L. Letter: Turista. N Engl J Med. 1975 Jul 10;293(2):101–102. doi: 10.1056/NEJM197507102930219. [DOI] [PubMed] [Google Scholar]
  11. Rosenberg M. L., Koplan J. P., Wachsmuth I. K., Wells J. G., Gangarosa E. J., Guerrant R. L., Sack D. A. Epidemic diarrhea at Crater Lake from enterotoxigenic Escherichia coli. A large waterborne outbreak. Ann Intern Med. 1977 Jun;86(6):714–718. doi: 10.7326/0003-4819-86-6-714. [DOI] [PubMed] [Google Scholar]
  12. Ryder R. W., Sack D. A., Kapikian A. Z., McLaughlin J. C., Chakraborty J., Mizanur Rahman A. S., Merson M. H., Wells J. G. Enterotoxigenic Escherichia coli and Reovirus-like agent in rural Bangladesh. Lancet. 1976 Mar 27;1(7961):659–663. doi: 10.1016/s0140-6736(76)92776-8. [DOI] [PubMed] [Google Scholar]
  13. Sack D. A., Sack R. B. Test for enterotoxigenic Escherichia coli using Y-1 adrenal cells in miniculture. Infect Immun. 1975 Feb;11(2):334–336. doi: 10.1128/iai.11.2.334-336.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sack R. B., Gorbach S. L., Banwell J. G., Jacobs B., Chatterjee B. D., Mitra R. C. Enterotoxigenic Escherichia coli isolated from patients with severe cholera-like disease. J Infect Dis. 1971 Apr;123(4):378–385. doi: 10.1093/infdis/123.4.378. [DOI] [PubMed] [Google Scholar]
  15. Sack R. B., Hirschborn N., Woodward W. E., Sack D. A., Cash R. A. Antibodies to heat-labile Escherichia coli enterotoxin in Apaches in Whiteriver, Arizona. Infect Immun. 1975 Dec;12(6):1475–1477. doi: 10.1128/iai.12.6.1475-1477.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sack R. B. Human diarrheal disease caused by enterotoxigenic Escherichia coli. Annu Rev Microbiol. 1975;29:333–353. doi: 10.1146/annurev.mi.29.100175.002001. [DOI] [PubMed] [Google Scholar]
  17. Sack R. B., Jacobs B., Mitra R. Antitoxin responses to infections with enterotoxigenic Escherichia coli. J Infect Dis. 1974 Mar;129(3):330–335. doi: 10.1093/infdis/129.3.330. [DOI] [PubMed] [Google Scholar]
  18. Sack R. B., Johnson J., Pierce N. F., Keren D. F., Yardley J. H. Challenge of dogs with live enterotoxigenic Escherichia coli and effects of repeated challenges on fluid secretion in jejunal Thiry-Vella loops. J Infect Dis. 1976 Jul;134(1):15–24. doi: 10.1093/infdis/134.1.15. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES