Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1977 Nov;18(2):386–392. doi: 10.1128/iai.18.2.386-392.1977

Mycoplasma-dependent activation of normal lymphocytes: role of arginine and nonviable mycoplasma antigen in the induction of lymphocyte-mediated cytotoxicity for syngeneic mouse target cells.

K E Aldridge, B C Cole, J R Ward
PMCID: PMC421244  PMID: 562854

Abstract

The ability of arginine supplementation to abrogate or potentiate Mycoplasma arthritidis-mediated cytotoxicity induction by normal CBA mouse lymphocytes was investigated. Also, nonviable antigens of M. arthritidis were assayed for their ability to induce a cytotoxic response. The presence of additional arginine in the culture medium had no significant effect on the amount of cytotoxicity produced. In some cases, an increase in cytotoxicity was seen in the presence of additional arginine. Amino acid analysis of the culture supernatants showed that the arginine in the nonsupplemented medium was exhausted during the assay, whereas ample amounts of arginine were present in the supplemented medium. However in both cases the amount of cytotoxicity was comparable. Nonviable antigens of M. arthritidis were unable to induce a significant cytotoxic response in the presence of normal lymphocytes. The same antigens exhibited a suppressive response when tested for blastogenesis in the presence of normal lymphocytes. A discussion of lymphocyte involvement is presented.

Full text

PDF
386

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aldridge K. E., Cole B. C., Ward J. R. Mycoplasma-dependent activation of normal lymphocytes: induction of a lymphocyte-mediated cytotoxicity for allogeneic and syngeneic mouse target cells. Infect Immun. 1977 Nov;18(2):377–385. doi: 10.1128/iai.18.2.377-385.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Andersson J., Sjöberg O., Möller G. Induction of immunoglobulin and antibody synthesis in vitro by lipopolysaccharides. Eur J Immunol. 1972 Aug;2(4):349–353. doi: 10.1002/eji.1830020410. [DOI] [PubMed] [Google Scholar]
  3. Biberfeld G., Gronowicz E. Mycoplasma pneumoniae is a polyclonal B-cell activator. Nature. 1976 May 20;261(5557):238–239. doi: 10.1038/261238a0. [DOI] [PubMed] [Google Scholar]
  4. Callewaert D. M., Kaplan J., Peterson W. D., Jr, Lightbody J. J. Suppression of lymphocyte activation by a factor produced by Mycoplasma arginini. J Immunol. 1975 Dec;115(6):1662–1664. [PubMed] [Google Scholar]
  5. Cohen I. R., Feldman M. The lysis of fibroblasts by lymphocytes sensitized in vitro: specific antigen activates a nonspecific effect. Cell Immunol. 1970 Nov;1(5):521–535. doi: 10.1016/0008-8749(70)90039-0. [DOI] [PubMed] [Google Scholar]
  6. Cole B. C., Aldridge K. E., Ward J. R. Mycoplasma-dependent activation of normal lymphocytes: mitogenic potential of mycoplasmas for mouse lymphocytes. Infect Immun. 1977 Nov;18(2):393–399. doi: 10.1128/iai.18.2.393-399.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cole B. C., Golightly-Rowland L., Ward J. R. Arthritis of mice induced by Mycoplasma arthritidis. Humoral antibody and lymphocyte responses of CBA mice. Ann Rheum Dis. 1976 Feb;35(1):14–22. doi: 10.1136/ard.35.1.14. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cole B. C., Golightly-Rowland L., Ward J. R. Arthritis of mice induced by Mycoplasma pulmonis: humoral antibody and lymphocyte responses of CBA mice. Infect Immun. 1975 Nov;12(5):1083–1092. doi: 10.1128/iai.12.5.1083-1092.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cole B. C., Overall J. C., Jr, Lombardi P. S., Glasgow L. A. Induction of interferon in ovine and human lymphocyte cultures by mycoplasmas. Infect Immun. 1976 Jul;14(1):88–94. doi: 10.1128/iai.14.1.88-94.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Copperman R., Morton H. E. Reversible inhibition of mitosis in lymphocyte cultures by non-viable Mycoplasma. Proc Soc Exp Biol Med. 1966 Dec;123(3):790–795. doi: 10.3181/00379727-123-31605. [DOI] [PubMed] [Google Scholar]
  11. Ginsburg H., Nicolet J. Extensive transformation of lymphocytes by a mycoplasma organism. Nat New Biol. 1973 Dec 5;246(153):143–146. doi: 10.1038/newbio246143a0. [DOI] [PubMed] [Google Scholar]
  12. Granger G. A., Williams T. W. Lymphocyte cytotoxicity in vitro: activation and release of a cytotoxic factor. Nature. 1968 Jun 29;218(5148):1253–1254. doi: 10.1038/2181253a0. [DOI] [PubMed] [Google Scholar]
  13. Janossy G., Greaves M. F. Lymphocyte activation. I. Response of T and B lymphocytes to phytomitogens. Clin Exp Immunol. 1971 Oct;9(4):483–498. [PMC free article] [PubMed] [Google Scholar]
  14. Kaklamanis E., Pavlatos M. The immunosuppressive effect of mycoplasma infection. I. Effect on the humoral and cellular response. Immunology. 1972 Apr;22(4):695–702. [PMC free article] [PubMed] [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  16. Lee S. H., Rozee K. R. Enhanced responsiveness of L-929 cells treated with interferon to arginine starvation. Exp Cell Res. 1975 Jun;93(1):142–151. doi: 10.1016/0014-4827(75)90433-4. [DOI] [PubMed] [Google Scholar]
  17. Ruddle N. H., Waksman B. H. Cytotoxicity mediated by soluble antigen and lymphocytes in delayed hypersensitivity. 3. Analysis of mechanism. J Exp Med. 1968 Dec 1;128(6):1267–1279. doi: 10.1084/jem.128.6.1267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Simberkoff M. S., Thorbecke G. J., Thomas L. Studies of PPLO infection. V. Inhibition of lymphocyte mitosis and antibody formation by mycoplasmal extracts. J Exp Med. 1969 Jun 1;129(6):1163–1181. doi: 10.1084/jem.129.6.1163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stanbridge E. Mycoplasmas and cell cultures. Bacteriol Rev. 1971 Jun;35(2):206–227. doi: 10.1128/br.35.2.206-227.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES