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. 1977 Nov;18(2):524–530. doi: 10.1128/iai.18.2.524-530.1977

Detection of hepatitis A antigen by immunofluorescence.

L R Mathiesen, S M Feinstone, R H Purcell, J A Wagner
PMCID: PMC421263  PMID: 200565

Abstract

Hepatitis A antigen (HA Ag) was demonstrated by immunofluorescence (IF) in liver biopsies from chimpanzees with experimental hepatitis A virus infection. Blocking experiments with paired sera from patients with hepatitis types A, B, or non-A, non-B, as well as with purified HA Ag, showed that the fluorescence was specific for HA Ag. HA Ag could be demonstrated only in biopsies from chimpanzees inoculated with hepatitis A virus. In two of four chimpanzees biopsied weekly, HA Ag could be detected by IF before stool shedding of HA Ag, elevation in serum alanine aminotransferase (SGPT), or histopathological evidence of liver disease was seen. The HA Ag was detected for 4 to 5 weeks; the last IF-positive biopsy was obtained after SGPT activity had returned to normal. In the two other chimpanzees, HA Ag could be detected only in the biopsy taken at the time of SGPT elevation. In the early IF-positive biopsies, HA Ag was diffusely distributed in the cytoplasm of many cells, but it later accumulated in a focal distribution in the cytoplasm of a few of the hepatocytes and Kupffer cells. This cytoplasmic distribution agrees with previous electron microscopic data.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alberti A., Realdi G., Tremolada F., Spina G. P. Liver cell surface localization of hepatitis B antigen and of immunoglobulins in acute and chronic hepatitis and in liver cirrhosis. Clin Exp Immunol. 1976 Sep;25(3):396–402. [PMC free article] [PubMed] [Google Scholar]
  2. Barker L. F., Chisari F. V., McGrath P. P., Dalgard D. W., Kirschstein R. L., Almeida J. D., Edington T. S., Sharp D. G., Peterson M. R. Transmission of type B viral hepatitis to chimpanzees. J Infect Dis. 1973 Jun;127(6):648–662. doi: 10.1093/infdis/127.6.648. [DOI] [PubMed] [Google Scholar]
  3. Boggs J. D., Melnick J. L., Conrad M. E., Felsher B. F. Viral hepatitis. Clinical and tissue culture studies. JAMA. 1970 Nov 9;214(6):1041–1046. doi: 10.1001/jama.214.6.1041. [DOI] [PubMed] [Google Scholar]
  4. CLARK H. F., SHEPARD C. C. A DIALYSIS TECHNIQUE FOR PREPARING FLUORESCENT ANTIBODY. Virology. 1963 Aug;20:642–644. doi: 10.1016/0042-6822(63)90292-7. [DOI] [PubMed] [Google Scholar]
  5. Dienstag J. L., Feinstone S. M., Purcell R. H., Hoofnagle J. H., Barker L. F., London W. T., Popper H., Peterson J. M., Kapikian A. Z. Experimental infection of chimpanzees with hepatitis A virus. J Infect Dis. 1975 Nov;132(5):532–545. doi: 10.1093/infdis/132.5.532. [DOI] [PubMed] [Google Scholar]
  6. Dienstag J. L., Routenberg J. A., Purcell R. H., Hooper R. R., Harrison W. O. Foodhandler-associated outbreak of hepatitis type A. An immune electron microscopic study. Ann Intern Med. 1975 Nov;83(5):647–650. doi: 10.7326/0003-4819-83-5-647. [DOI] [PubMed] [Google Scholar]
  7. Edgington T. S., Ritt D. J. Intrahepatic expression of serum hepatitis virus-associated antigens. J Exp Med. 1971 Oct 1;134(4):871–885. doi: 10.1084/jem.134.4.871. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Feinstone S. M., Kapikian A. Z., Purceli R. H. Hepatitis A: detection by immune electron microscopy of a viruslike antigen associated with acute illness. Science. 1973 Dec 7;182(4116):1026–1028. doi: 10.1126/science.182.4116.1026. [DOI] [PubMed] [Google Scholar]
  9. Feinstone S. M., Kapikian A. Z., Purcell R. H., Alter H. J., Holland P. V. Transfusion-associated hepatitis not due to viral hepatitis type A or B. N Engl J Med. 1975 Apr 10;292(15):767–770. doi: 10.1056/NEJM197504102921502. [DOI] [PubMed] [Google Scholar]
  10. Hollinger F. B., Bradley D. W., Maynard J. E., Dreesman G. R., Melnick J. L. Detection of hepatitis A viral antigen by radioimmunoassay. J Immunol. 1975 Nov;115(5):1464–1466. [PubMed] [Google Scholar]
  11. Holmes A. W., Wolfe L., Rosenblate H., Deinhardt F. Hepatitis in marmosets: induction of disease with coded specimens from a human volunteer study. Science. 1969 Aug 22;165(3895):816–817. doi: 10.1126/science.165.3895.816. [DOI] [PubMed] [Google Scholar]
  12. Maynard J. E., Bradley D. W., Gravelle C. R., Ebert J. W., Krushak D. H. Preliminary studies of hepatitis A in chimpanzees. J Infect Dis. 1975 Feb;131(2):194–197. doi: 10.1093/infdis/131.2.194. [DOI] [PubMed] [Google Scholar]
  13. Miller W. J., Provost P. J., McAleer W. J., Ittensohn O. L., Villarejos V. M., Hilleman M. R. Specific immune adherence assay for human hepatitis A antibody application to diagnostic and epidemiologic investigations. Proc Soc Exp Biol Med. 1975 May;149(1):254–261. doi: 10.3181/00379727-149-38783. [DOI] [PubMed] [Google Scholar]
  14. Moritsugu Y., Dienstag J. L., Valdesuso J., Wong D. C., Wagner J., Routenberg J. A., Purcell R. H. Purification of hepatitis A antigen from feces and detection of antigen and antibody by immune adherence hemagglutination. Infect Immun. 1976 Mar;13(3):898–908. doi: 10.1128/iai.13.3.898-908.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Murphy B. L., Peterson J. M., Ebert J. W., Berquist K. R., Maynard J. E., Purcell R. H. Immunofluorescent localization of hepatitis B antigens in chimpanzee tissues. Intervirology. 1975;6(4-5):207–211. doi: 10.1159/000149475. [DOI] [PubMed] [Google Scholar]
  16. Nowoslawski A., Krawczyński K., Brzosko W. J., Madaliński K. Tissue localization of Australia antigen immune complexes in acute and chronic hepatitis and liver cirrhosis. Am J Pathol. 1972 Jul;68(1):31–56. [PMC free article] [PubMed] [Google Scholar]
  17. Provost P. J., Ittensohn O. L., Villarejos V. M., Hilleman M. R. A specific complement-fixation test for human hepatitis a employing CR326 virus antigen. Diagnosis and epidemiology. Proc Soc Exp Biol Med. 1975 Apr;148(4):962–969. doi: 10.3181/00379727-148-38669. [DOI] [PubMed] [Google Scholar]
  18. Provost P. J., Wolanski B. S., Miller W. J., Ittensohn O. L., McAleer W. J., Hilleman M. R. Physical, chemical and morphologic dimensions of human hepatitis A virus strain CR326 (38578). Proc Soc Exp Biol Med. 1975 Feb;148(2):532–539. doi: 10.3181/00379727-148-38578. [DOI] [PubMed] [Google Scholar]
  19. Purcell R. H., Wong D. C., Alter H. J., Holland P. V. Microtiter solid-phase radioimmunoassay for hepatitis B antigen. Appl Microbiol. 1973 Oct;26(4):478–484. doi: 10.1128/am.26.4.478-484.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Purcell R. H., Wong D. C., Moritsugu Y., Dienstag J. L., Routenberg J. A., Boggs J. D. A microtiter solid-phase radioimmunoassay for hepatitis A antigen and antibody. J Immunol. 1976 Feb;116(2):349–356. [PubMed] [Google Scholar]
  21. Rakela J., Stevenson D., Edwards V. M., Gordon I., Mosley J. W. Antibodies to hepatitis A virus: patterns by two procedures. J Clin Microbiol. 1977 Jan;5(1):110–111. doi: 10.1128/jcm.5.1.110-111.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Schulman A. N., Dienstag J. L., Jackson D. R., Hoofnagle J. H., Gerety R. J., Purcell R. H., Barker L. F. Hepatitis A antigen particles in liver, bile, and stool of chimpanzees. J Infect Dis. 1976 Jul;134(1):80–84. doi: 10.1093/infdis/134.1.80. [DOI] [PubMed] [Google Scholar]

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