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. 1977 Dec;18(3):746–753. doi: 10.1128/iai.18.3.746-753.1977

Effect of alveolar macrophages on Toxoplasma gondii.

F W Ryning, J S Remington
PMCID: PMC421298  PMID: 591065

Abstract

As pulmonary involvement can occur in disseminated toxoplasmosis in immunosuppressed patients, studies were initiated to define local mechanisms of resistance of the lung to Toxoplasma gondii. Alveolar macrophages were obtained from normal mice and mice chronically infected with T. gondii by bronchopulmonary lavage and cultured in vitro. Although normal alveolar macrophages were difficult to infect with Toxoplasma, they supported intracellular multiplication of this organism. When exposed to Toxoplasma that had been pretreated with heat-inactivated serum containing specific antibody, the number of intracellular organisms increased remarkably, and the macrophages destroyed the coated parasites. After development of chronic infections with Toxoplasma, there was a transient period during which a striking increase in numbers of alveolar macrophages was observed in lavage specimens. These macrophages differed from those of normal alveolar macrophages. There was a greater percentage of large cells, a greater tendency to spread on glass, and an increased number of intracellular Toxoplasma, and the cells were activated to kill or inhibit multiplication of the parasite. During the period when activated macrophages were demonstrable in bronchopulmonary washings, histological changes in the lungs revealed a marked mononuclear cell infiltrate. These studies support a role for the activated alveolar macrophage as an effector in resistance of the lung to infection with Toxoplasma.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson S. E., Jr, Bautista S. C., Remington J. S. Specific antibody-dependent killing of Toxoplasma gondii by normal macrophages. Clin Exp Immunol. 1976 Dec;26(3):375–380. [PMC free article] [PubMed] [Google Scholar]
  2. Anderson S. E., Jr, Remington J. S. Effect of normal and activated human macrophages on Toxoplasma gondii. J Exp Med. 1974 May 1;139(5):1154–1174. doi: 10.1084/jem.139.5.1154. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Borges J. S., Johnson W. D., Jr Inhibition of multiplication of Toxoplasma gondii by human monocytes exposed to T-lymphocyte products. J Exp Med. 1975 Feb 1;141(2):483–496. doi: 10.1084/jem.141.2.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bulmer G. S., Tacker J. R. Phagocytosis of Cryptococcus neoformans by alveolar macrophages. Infect Immun. 1975 Jan;11(1):73–79. doi: 10.1128/iai.11.1.73-79.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cantey J. R., Hand W. L. Cell-mediated immunity after bacterial infection of the lower respiratory tract. J Clin Invest. 1974 Nov;54(5):1125–1134. doi: 10.1172/JCI107856. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cohen A. B., Cline M. J. The human alveolar macrophage: isolation, cultivation in vitro, and studies of morphologic and functional characteristics. J Clin Invest. 1971 Jul;50(7):1390–1398. doi: 10.1172/JCI106622. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dannenberg A. M., Jr Cellular hypersensitivity and cellular immunity in the pathogensis of tuberculosis: specificity, systemic and local nature, and associated macrophage enzymes. Bacteriol Rev. 1968 Jun;32(2):85–102. doi: 10.1128/br.32.2.85-102.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. De Sanchez S. B., Carbonell L. M. Immunological studies on Histoplasma capsulatum. Infect Immun. 1975 Feb;11(2):387–394. doi: 10.1128/iai.11.2.387-394.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. FRENKEL J. K., JACOBS L. Ocular toxoplasmosis; pathogenesis, diagnosis and treatment. AMA Arch Ophthalmol. 1958 Feb;59(2):260–279. [PubMed] [Google Scholar]
  10. Frenkel J. K. Adoptive immunity to intracellular infection. J Immunol. 1967 Jun;98(6):1309–1319. [PubMed] [Google Scholar]
  11. GOLDMAN M. Staining Toxoplasma gondii with fluorescein-labelled antibody. I. The reaction in smears of peritoneal exudate. J Exp Med. 1957 Jun 1;105(6):549–556. doi: 10.1084/jem.105.6.549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gentry L. O., Remington J. S. Resistance against Cryptococcus conferred by intracellular bacteria and protozoa. J Infect Dis. 1971 Jan;123(1):22–31. doi: 10.1093/infdis/123.1.22. [DOI] [PubMed] [Google Scholar]
  13. Gleason T. H., Hamlin W. B. Disseminated toxoplasmosis in the compromised host. A report of five cases. Arch Intern Med. 1974 Dec;134(6):1059–1062. [PubMed] [Google Scholar]
  14. Hoff R. L., Frenkel J. K. Cell-mediated immunity against Besnoitia and toxoplasma in specifically and cross-immunized hamsters and in cultures. J Exp Med. 1974 Mar 1;139(3):560–580. doi: 10.1084/jem.139.3.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Johnson J. D., Hand W. L., King N. L., Hughes C. G. Activation of alveolar macrophages after lower respiratory tract infection. J Immunol. 1975 Jul;115(1):80–84. [PubMed] [Google Scholar]
  16. Jones T. C., Hirsch J. G. The interaction between Toxoplasma gondii and mammalian cells. II. The absence of lysosomal fusion with phagocytic vacuoles containing living parasites. J Exp Med. 1972 Nov 1;136(5):1173–1194. doi: 10.1084/jem.136.5.1173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Jones T. C., Len L., Hirsch J. G. Assessment in vitro of immunity against Toxoplasma gondii. J Exp Med. 1975 Feb 1;141(2):466–482. doi: 10.1084/jem.141.2.466. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Jones T. C., Yeh S., Hirsch J. G. The interaction between Toxoplasma gondii and mammalian cells. I. Mechanism of entry and intracellular fate of the parasite. J Exp Med. 1972 Nov 1;136(5):1157–1172. doi: 10.1084/jem.136.5.1157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. KAPLOW L. S. SIMPLIFIED MYELOPEROXIDASE STAIN USING BENZIDINE DIHYDROCHLORIDE. Blood. 1965 Aug;26:215–219. [PubMed] [Google Scholar]
  20. Kaltreider H. B. Expression of immune mechanisms in the lung. Am Rev Respir Dis. 1976 Mar;113(3):347–379. doi: 10.1164/arrd.1976.113.3.347. [DOI] [PubMed] [Google Scholar]
  21. Myrvik Q. N. Function of the alveolar macrophage in immunity. J Reticuloendothel Soc. 1972 May;11(5):459–468. [PubMed] [Google Scholar]
  22. North R. J., Mackaness G. B., Elliott R. W. The histogenesis of immunologically committed lymphocytes. Cell Immunol. 1972 Apr;3(4):680–694. doi: 10.1016/0008-8749(72)90130-x. [DOI] [PubMed] [Google Scholar]
  23. Remington J. S., Krahenbuhl J. L., Mendenhall J. W. A role for activated macrophages in resistance to infection with Toxoplasma. Infect Immun. 1972 Nov;6(5):829–834. doi: 10.1128/iai.6.5.829-834.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Reynolds H. Y., Atkinson J. P., Newball H. H., Frank M. M. Receptors for immunoglobulin and complement on human alveolar macrophages. J Immunol. 1975 Jun;114(6):1813–1819. [PubMed] [Google Scholar]
  25. Reynolds H. Y., Kazmierowski J. A., Newball H. H. Specificity of opsonic antibodies to enhance phagocytosis of Pseudomonas aeruginosa by human alveolar macrophages. J Clin Invest. 1975 Aug;56(2):376–385. doi: 10.1172/JCI108102. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Ruskin J., Remington J. S. Toxoplasmosis in the compromised host. Ann Intern Med. 1976 Feb;84(2):193–199. doi: 10.7326/0003-4819-84-2-193. [DOI] [PubMed] [Google Scholar]
  27. Ruskin J., Rengton J. S. Role for the macrophage in acquired immunity to phylogenetically unrelated intracellular organisms. Antimicrob Agents Chemother (Bethesda) 1968;8:474–477. doi: 10.1128/AAC.8.4.474. [DOI] [PubMed] [Google Scholar]
  28. Truitt G. L., Mackaness G. B. Cell-mediated resistance to aerogenic infection of the lung. Am Rev Respir Dis. 1971 Dec;104(6):829–843. doi: 10.1164/arrd.1971.104.6.829. [DOI] [PubMed] [Google Scholar]
  29. VISCHER W. A., SUTER E. Intracellular multiplication of Toxoplasma gondi in adult mammalian macrophages cultivated in vitro. Proc Soc Exp Biol Med. 1954 Jul;86(3):413–419. doi: 10.3181/00379727-86-21117. [DOI] [PubMed] [Google Scholar]
  30. Vietzke W. M., Gelderman A. H., Grimley P. M., Valsamis M. P. Toxoplasmosis complicating malignancy. Experience at the National Cancer Institute. Cancer. 1968 May;21(5):816–827. doi: 10.1002/1097-0142(196805)21:5<816::aid-cncr2820210506>3.0.co;2-#. [DOI] [PubMed] [Google Scholar]
  31. Yam L. T., Li C. Y., Crosby W. H. Cytochemical identification of monocytes and granulocytes. Am J Clin Pathol. 1971 Mar;55(3):283–290. doi: 10.1093/ajcp/55.3.283. [DOI] [PubMed] [Google Scholar]

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