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. 1977 Dec;18(3):806–815. doi: 10.1128/iai.18.3.806-815.1977

Epidemic caprine keratoconjunctivitis: recovery of Mycoplasma conjunctivae and its possible role in pathogenesis.

E J Baas, S L Trotter, R M Franklin, M F Barile
PMCID: PMC421305  PMID: 591066

Abstract

Clinical, microbiological, serological, histological, and therapeutic aspects of two separate outbreaks of caprine keratoconjunctivitis are described. The disease was characterized by a high rate of contagion, rapid onset, intense lacrimation, conjunctival hyperemia, and corneal opacity with neovascularization. In addition, many of the animals developed respiratory illness during the second epidemic. The only organism consistentlyisolated was Mycoplasma conjunctivae. A total of 23 strains were isolated from 18 inflamed conjunctivae, one normal conjunctiva, and the nasal secretions of four goats with concomitant respiratory illness. The convalescent sera of goats in the first outbreak had neutralizing antibody titers to M. conjunctivae that ranged from 1:32 to 1:256. In the milder second outbreak the antibody titers ranged from 1:4 to 1:32 in animals with only ocular disease and from 1:4 to 1:64 in animals with only respiratory disease. Whereas little change was noted in antibody titers of goats with only localized eye disease, 43% of the goats with respiratory disease showed significant fourfold rises. The histological picture was consistent with acute corneal infection. Animals requiring antibiotic treatment appeared to respond favorably to a combination of oxytetracycline and polymyxin B, but not to penicillin. These findings suggest that M. conjunctivae is one cause of epidemic caprine keratoconjunctivitis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barile M. F., Del Giudice R. A., Tully J. G. Isolation and characterization of Mycoplasma conjunctivae sp. n. from sheep and goats with keratoconjunctivitis. Infect Immun. 1972 Jan;5(1):70–76. doi: 10.1128/iai.5.1.70-76.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cole B. C., Golightly L., Ward J. R. Characterization of mycoplasma strains from cats. J Bacteriol. 1967 Nov;94(5):1451–1458. doi: 10.1128/jb.94.5.1451-1458.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Del Giudice R. A., Robillard N. F., Carski T. R. Immunofluorescence identification of Mycoplasma on agar by use of incident illumination. J Bacteriol. 1967 Apr;93(4):1205–1209. doi: 10.1128/jb.93.4.1205-1209.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. HOLLAND M. C., WORLTON J. T., Jr Relationship of pleuropneumonialike organisms and uveitis. Am J Ophthalmol. 1957 Apr;43(4 Pt 1):597–606. doi: 10.1016/0002-9394(57)90812-7. [DOI] [PubMed] [Google Scholar]
  5. Hudson J. R., Cottew G. S., Adler H. E. Diseases of goats caused by mycoplasma: a review of the subject with some new findings. Ann N Y Acad Sci. 1967 Jul 28;143(1):287–297. doi: 10.1111/j.1749-6632.1967.tb27668.x. [DOI] [PubMed] [Google Scholar]
  6. Jonas A. M., Barber T. L. Mycoplasma mycoides var, capri isolated from a goat in Connecticut. J Infect Dis. 1969 Feb;119(2):126–131. doi: 10.1093/infdis/119.2.126. [DOI] [PubMed] [Google Scholar]
  7. Klingler K., Nicolet J., Schipper E. Neue Befunde über die Gemsblindheit. Schweiz Arch Tierheilkd. 1969 Oct;111(10):587–602. [PubMed] [Google Scholar]
  8. Langford E. V., Leach R. H. Characterization of a mycoplasma isolated from infectious bovine keratoconjunctivitis: M. bovoculi sp. nov. Can J Microbiol. 1973 Nov;19(11):1435–1444. doi: 10.1139/m73-231. [DOI] [PubMed] [Google Scholar]
  9. Langford E. V. Mycoplasma and associated bacteria isolated from ovine pink-eye. Can J Comp Med. 1971 Jan;35(1):18–21. [PMC free article] [PubMed] [Google Scholar]
  10. NEWNHAM A. G., CHU H. P. AN IN VITRO COMPARISON OF THE EFFECT OF SOME ANTIBACTERIAL, ANTIFUNGAL AND ANTIPROTOZOAL AGENTS ON VARIOUS STRAINS OF MYCOPLASMA (PLEUROPNEUMONIA-LIKE ORGANISMS: P.P.L.O.). J Hyg (Lond) 1965 Mar;63:1–23. doi: 10.1017/s0022172400044922. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nicolet J., Dauwalder M., Boss P. H., Anetzhofer J. Die "primär" infektiöse Keratoconjunctivitis des Rindes. Mögliche ätiologische Rolle von Mycoplasma bovoculi. Schweiz Arch Tierheilkd. 1976 Apr;118(4):141–150. [PubMed] [Google Scholar]
  12. Nicolet J., Freundt E. A. Isolation of mycoplasma conjunctivae from Chamois and sheep affected with kerato-conjunctivitis. Zentralbl Veterinarmed B. 1975 Jun;22(4):302–307. doi: 10.1111/j.1439-0450.1975.tb00591.x. [DOI] [PubMed] [Google Scholar]
  13. Surman P. G. Cytology of "pink-eye" of sheep, including a reference to trachoma of man, by employing acridine orange and iodine stains, and isolation of Mycoplasma agents from infected sheep eyes. Aust J Biol Sci. 1968 Jun;21(3):447–467. doi: 10.1071/bi9680447. [DOI] [PubMed] [Google Scholar]
  14. Surman P. G. Mycoplasma aetiology of keratoconjunctivitis ("pink-eye") in domestic ruminants. Aust J Exp Biol Med Sci. 1973 Oct;51(5):589–607. doi: 10.1038/icb.1973.56. [DOI] [PubMed] [Google Scholar]
  15. Taylor-Robinson D., Purcell R. H., Wong D. C., Chanock R. M. A colour test for the measurement of antibody to certain mycoplasma species based upon the inhibition of acid production. J Hyg (Lond) 1966 Mar;64(1):91–104. doi: 10.1017/s0022172400040377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Trotter S. L., Franklin R. M., Baas E. J., Barile M. F. Epidemic caprine keratoconjunctivitis: experimentally induced disease with a pure culture of Mycoplasma conjunctivae. Infect Immun. 1977 Dec;18(3):816–822. doi: 10.1128/iai.18.3.816-822.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. al-Aubaidi J. M., Dardiri A. H., Muscoplatt C. C., McCauley E. H. Identification and characterization of Acholeplasma oculusi spec. nov. from the eyes of goats with keratoconjunctivitis. Cornell Vet. 1973 Jan;63(1):117–129. [PubMed] [Google Scholar]

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