Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1977 Feb;15(2):453–460. doi: 10.1128/iai.15.2.453-460.1977

Immunoprotective activity of ribosomes from Haemophilus influenzae.

M Lynn, R P Tewari, M Solotorovsky
PMCID: PMC421389  PMID: 300360

Abstract

Immunization with ribosomal preparations from Haemophilus influenzae type b elicited protective immunity in mice. Ribosomes from disrupted cells where isolated by differential centrifugation using sodium dodecyl sulfate. The washed ribosomes contained 25% protein and 75% ribonucleic acid and sedimented as a single peak on sucrose density gradient analysis with a sedimentation coefficient of 67S, using Escherichia coli ribosomes as a 70S marker. Immunodiffusion tests with antipolyribose phosphate serum showed that the ribosomes were free from capsular material. Mice immunized subcutaneously with ribosomes, with or without adjuvant, were challenged intraperitoneally with 100 to 1,000 mean lethal doses of H. influenzae type b suspended in gastric mucin. Significant protection was induced by ribosomes and was compared to that obtained after sublethal infection with live cells. The protection was greatly enhanced after incorporation of ribosomes into adjuvants. Maximum protection (90 to 95%) was observed at 1 to 2 weeks after immunization. Ribosomes from a nonencapsulated strain of H. influenzae were as immunogenic as those from the encapsulated strain, demonstrating that the capsular material is not responsible for immunogenicity of Haemophilus ribosomes.

Full text

PDF
453

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson P., Johnston R. B., Jr, Smith D. H. Human serum activities against Hemophilus influenzae, type b. J Clin Invest. 1972 Jan;51(1):31–38. doi: 10.1172/JCI106793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Andron LA I. I., Eigelsbach H. T. Biochemical and immunological properties of ribonucleic acid-rich extracts from Francisella tularensis. Infect Immun. 1975 Jul;12(1):137–142. doi: 10.1128/iai.12.1.137-142.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BROWN H. M., WILSON R. N. Chronic bronchitis in industry; an account of a trial of H. influenzae vaccine. Br Med J. 1959 Jan 31;1(5117):263–267. doi: 10.1136/bmj.1.5117.263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Collins F. M. Mechanisms in antimicrobial immunity. J Reticuloendothel Soc. 1971 Jul;10(1):58–99. [PubMed] [Google Scholar]
  5. EDWARDS G., BUCKLEY A. R., FEAR E. C., WILLIAMSON G. M., ZINNEMANN K. Adult chronic bronchitis; the infective factor and its treatment. Br Med J. 1957 Aug 3;2(5039):259–264. doi: 10.1136/bmj.2.5039.259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Feit C., Tewari R. P. Immunogenicity of Ribosomal Preparations from Yeast Cells of Histoplasma capsulatum. Infect Immun. 1974 Nov;10(5):1091–1097. doi: 10.1128/iai.10.5.1091-1097.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HILLEMAN M. R. A FORWARD LOOK AT VIRAL VACCINES: WITH SPECIAL REFERENCE TO A NEW IMMUNOLOGIC ADJUVANT. Am Rev Respir Dis. 1964 Nov;90:683–706. doi: 10.1164/arrd.1964.90.5.683. [DOI] [PubMed] [Google Scholar]
  8. Handzel Z. T., Argaman M., Parke J. C., Jr, Schneerson R., Robbins J. B. Heteroimmunization to the capsular polysaccharide of Haemophilus influenzae type b induced by enteric cross-reacting bacteria. Infect Immun. 1975 May;11(5):1045–1052. doi: 10.1128/iai.11.5.1045-1052.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jensen R., Gregory B., Naylor J., Actor P. Isolation of protective somatic antigen from Vibrio cholerae (Ogawa) ribosomal preparations. Infect Immun. 1972 Aug;6(2):156–161. doi: 10.1128/iai.6.2.156-161.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Johnson W. Ribosomal vaccines. I. Immunogenicity of ribosomal fractions isolated from Salmonella typhimurium and Yersinia pestis. Infect Immun. 1972 Jun;5(6):947–952. doi: 10.1128/iai.5.6.947-952.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Johnson W. Ribosomal vaccines. II. Specificity of the immune response to ribosomal ribonucleic acid and protein isolated from Salmonella typhimurium. Infect Immun. 1973 Sep;8(3):395–400. doi: 10.1128/iai.8.3.395-400.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. MARTIN R. G., AMES B. N. A method for determining the sedimentation behavior of enzymes: application to protein mixtures. J Biol Chem. 1961 May;236:1372–1379. [PubMed] [Google Scholar]
  14. Michaels R. H. Increase in influenzal meningitis. N Engl J Med. 1971 Sep 16;285(12):666–667. doi: 10.1056/NEJM197109162851207. [DOI] [PubMed] [Google Scholar]
  15. NYHAN W. L., RICHARDSON F. Complications of meningitis. Annu Rev Med. 1963;14:243–260. doi: 10.1146/annurev.me.14.020163.001331. [DOI] [PubMed] [Google Scholar]
  16. Preston P. M., Dumonde D. C. Immunogenicity of a ribosomal antigen of Leishmania enriettii. Trans R Soc Trop Med Hyg. 1971;65(1):18–19. [PubMed] [Google Scholar]
  17. Rodrigues L. P., Schneerson R., Robbins J. B. Immunity to Hemophilus influenzae type b. I. The isolation, and some physicochemical, serologic and biologic properties of the capsular polysaccharide of Hemophilus influenzae type b. J Immunol. 1971 Oct;107(4):1071–1080. [PubMed] [Google Scholar]
  18. Schalla W. O., Johnson W. Immunogenicity of ribosomal vaccines isolated from group A, type 14 Streptococcus pyogenes. Infect Immun. 1975 Jun;11(6):1195–1202. doi: 10.1128/iai.11.6.1195-1202.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schneerson R., Rodrigues L. P., Parke J. C., Jr, Robbins J. B. Immunity to disease caused by Hemophilus influenzae type b. II. Specificity and some biologic characteristics of "natural," infection-acquired, and immunization-induced antibodies to the capsular polysaccharide of Hemophilus influenzae type b. J Immunol. 1971 Oct;107(4):1081–1089. [PubMed] [Google Scholar]
  20. Sell S. H., Merrill R. E., Doyne E. O., Zimsky E. P., Jr Long-term sequelae of Hemophilus influenzae meningitis. Pediatrics. 1972 Feb;49(2):206–211. [PubMed] [Google Scholar]
  21. Smith A. L., Smith D. H., Averill D. R., Jr, Marino J., Moxon E. R. Production of Haemophilus influenzae b meningitis in infant rats by intraperitoneal inoculation. Infect Immun. 1973 Aug;8(2):278–290. doi: 10.1128/iai.8.2.278-290.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Smith E. W., Jr, Haynes R. E. Changing incidence of Hemophilus influenzae meningitis. Pediatrics. 1972 Nov;50(5):723–727. [PubMed] [Google Scholar]
  23. Smith R. A., Bigley N. J. Ribonucleic acid-protein fractions of virulent Salmonella typhimurium as protective immunogens. Infect Immun. 1972 Sep;6(3):377–383. doi: 10.1128/iai.6.3.377-383.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Swendsen C. L., Johnson W. Humoral immunity to Streptococcus pneumoniae induced by a pneumococcal ribosomal protein fraction. Infect Immun. 1976 Aug;14(2):345–354. doi: 10.1128/iai.14.2.345-354.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Thomas D. W., Weiss E. Response of mice to injection of ribosomal fraction from group B Neisseria meningitidis. Infect Immun. 1972 Sep;6(3):355–363. doi: 10.1128/iai.6.3.355-363.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Thompson H. C., Eisenstein T. K. Biological properties of an immunogenic pneumococcal subcellular preparation. Infect Immun. 1976 Mar;13(3):750–757. doi: 10.1128/iai.13.3.750-757.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Thompson H. C., Snyder I. S. Protection against pneumococcal infection by a ribosomal preparation. Infect Immun. 1971 Jan;3(1):16–23. doi: 10.1128/iai.3.1.16-23.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Venneman M. R., Bigley N. J., Berry L. J. Immunogenicity of Ribonucleic Acid Preparations Obtained from Salmonella typhimurium. Infect Immun. 1970 Jun;1(6):574–582. doi: 10.1128/iai.1.6.574-582.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Wehrle P. F., Mathies A. W., Jr, Leedom J. M. The critically ill child: management of acute bacterial meningitis. Pediatrics. 1969 Dec;44(6):991–998. [PubMed] [Google Scholar]
  30. Winston S. H., Berry L. J. Antibacterial immunity induced by ribosomal vaccines. J Reticuloendothel Soc. 1970 Jul;8(1):13–24. [PubMed] [Google Scholar]
  31. Winston S., Berry L. J. Immunity induced by ribosomal extracts from Staphylococcus aureus. J Reticuloendothel Soc. 1970 Jul;8(1):66–73. [PubMed] [Google Scholar]
  32. YOUMANS A. S., YOUMANS G. P. IMMUNOGENIC ACTIVITY OF A RIBOSOMAL FRACTION OBTAINED FROM MYCOBACTERIUM TUBERCULOSIS. J Bacteriol. 1965 May;89:1291–1298. doi: 10.1128/jb.89.5.1291-1298.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Youmans A. S., Youmans G. P. Factors affecting immunogenic activity of mycobacterial ribosomal and ribonucleic acid preparations. J Bacteriol. 1969 Jul;99(1):42–50. doi: 10.1128/jb.99.1.42-50.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Youmans A. S., Youmans G. P. Preparation and effect of different adjuvants on the immunogenic activity of mycobacterial ribosomal fraction. J Bacteriol. 1967 Oct;94(4):836–843. doi: 10.1128/jb.94.4.836-843.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES