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. 1977 Feb;15(2):478–484. doi: 10.1128/iai.15.2.478-484.1977

Effect of rheumatoid factor and anti-immunoglobulin G antibodies on complement-mediated lysis of herpes simplex virus-infected human fibroblasts.

J C Shivers, C A Daniels
PMCID: PMC421393  PMID: 191401

Abstract

The effect of various anti-immunoglobulin G (IgG) antibodies on the complement-mediated lysis of herpes simplex virus-infected human fibroblasts was determined. IgM rheumatoid factor, a naturally occurring anti-human Fc, inhibited lysis, whereas rabbit anti-human IgG serum potentiated immune cytolysis. We attempted to explain this disparity by determining the effect various classess of anti-IgG's with differing specificities had on complement-mediated lysis. Inhibition of cytolysis occurred with IgM anti-Fc and all of the IgG antiglobulins (anti-IgG, Fab, and Fc). In contrast, IgM anti-Fab enhanced lysis. IgM anti-IgG suppressed immune cytolysis when high concentrations of antiviral serum were incubated with the virus-infected cell, but augmented lysis when low concentrations of anti-herpes simplex virus antibody were exposed to the fibroblasts. The experiments indicated that whether a particular antiglobulin potentiates or inhibits lysis depends on the concentration of antibody bound to the target cells as well as the class and specificity of the antiglobulin exposed to the antibody-coated cell.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashe W. K., Notkins A. L. Neutralization of an infectious herpes simplex virus-antibody complex by anti-gamma-globulin. Proc Natl Acad Sci U S A. 1966 Aug;56(2):447–451. doi: 10.1073/pnas.56.2.447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BALL J., LAWRENCE J. S. Epidemiology of the sheep cell agglutination test. Ann Rheum Dis. 1961 Sep;20:235–243. doi: 10.1136/ard.20.3.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bianco N. E., Dobkin L. W., Schur P. H. Immunological properties of isolated IgG and IgM anti-gamma-globulins (rheumatoid factors). Clin Exp Immunol. 1974 May;17(1):91–101. [PMC free article] [PubMed] [Google Scholar]
  4. Borsos T., Rapp H. J. Complement fixation on cell surfaces by 19S and 7S antibodies. Science. 1965 Oct 22;150(3695):505–506. doi: 10.1126/science.150.3695.505. [DOI] [PubMed] [Google Scholar]
  5. Borsos T., Rapp H. J. Hemolysin titration based on fixation of the activated first component of complement: evidence that one molecule of hemolysin suffices to sensitize an erythrocyte. J Immunol. 1965 Sep;95(3):559–566. [PubMed] [Google Scholar]
  6. Boyle M. D., Ohanian S. H., Borsos T. Studies on the terminal stages of antibody-complement-mediated killing of a tumor cell. II. Inhibition of transformation of T to dead cells by 3'5' cAMP. J Immunol. 1976 May;116(5):1276–1279. [PubMed] [Google Scholar]
  7. Brier A. M., Wohlenberg C., Rosenthal J., Mage M., Notkins A. L. Inhibition or enhancement of immunological injury of virus-infected cells. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3073–3077. doi: 10.1073/pnas.68.12.3073. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Burakoff S. J., Martz E., Benacerraf B. Is the primary complement lesion insufficient for lysis? Failure of cells damaged under osmotic protection to lyse in EDTA or at low temperature after removal of osmotic protection. Clin Immunol Immunopathol. 1975 May;4(1):108–126. doi: 10.1016/0090-1229(75)90045-8. [DOI] [PubMed] [Google Scholar]
  9. Capra J. D., Winchester R. J., Kunkel H. G. Cold-reactive rheumatoid factors in infectious mononucleosis and other diseases. Arthritis Rheum. 1969 Apr;12(2):67–73. doi: 10.1002/art.1780120202. [DOI] [PubMed] [Google Scholar]
  10. Dudley F. J., O'Shea M. J., Sherlock S. Serum autoantibodies in hepatitis associated antigen (HAA) positive patients. A possible index of cell-mediated immunity to the associated-infective agent. Clin Exp Immunol. 1973 Mar;13(3):367–372. [PMC free article] [PubMed] [Google Scholar]
  11. FAHEY J. L., McCOY P. F., GOULIAN M. Chromatography of serum proteins in normal and pathologic sera: the distribution of protein-bound carbohydrate and cholesterol, siderophilin, thyroxin-binding protein, B12-binding protein, alkaline and acid phosphatases, radio-iodinated albumin and myeloma proteins. J Clin Invest. 1958 Feb;37(2):272–284. doi: 10.1172/JCI103606. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. FRANKLIN E. C. Structural units of human 7S gamma globulin. J Clin Invest. 1960 Dec;39:1933–1941. doi: 10.1172/JCI104218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gipson T. G., Daniels C. A., Notkins A. L. Interaction of rheumatoid factor with infectious vaccinia virus-antibody complexes. J Immunol. 1974 Jun;112(6):2087–2093. [PubMed] [Google Scholar]
  14. Gipson T. G., Daniels C. A. The effect of rheumatoid factor on the immune lysis of vaccinia virus-infected cells. Clin Immunol Immunopathol. 1975 May;4(1):16–23. doi: 10.1016/0090-1229(75)90034-3. [DOI] [PubMed] [Google Scholar]
  15. Goetzl E. J., Metzger H. Affinity labeling of a mouse myeloma protein which binds nitrophenyl ligands. Kinetics of labeling and isolation of a labeled peptide. Biochemistry. 1970 Mar 3;9(5):1267–1278. doi: 10.1021/bi00807a031. [DOI] [PubMed] [Google Scholar]
  16. HEIMER R., LEVIN F. M., RUDD E. GLOBULINS RESEMBLING RHEUMATOID FACTOR IN SERUM OF THE AGED. Am J Med. 1963 Aug;35:175–181. doi: 10.1016/0002-9343(63)90208-0. [DOI] [PubMed] [Google Scholar]
  17. HOWELL D. S., MALCOLM J. M., PIKE R. The F-II agglutinating factors in serums of patients with non-rheumatic diseases. Am J Med. 1960 Oct;29:662–671. doi: 10.1016/0002-9343(60)90099-1. [DOI] [PubMed] [Google Scholar]
  18. Hampar B., Notkins A. L., Mage M., Keehn M. A. Heterogeneity in the properties of 7 S and 19S rabbit-neutralizing antibodies to herpes simplex virus. J Immunol. 1968 Mar;100(3):586–593. [PubMed] [Google Scholar]
  19. Humphrey J. H. Haemolytic efficiency of rabbit IgG anti-Forssman antibody and its augmentation by anti-rabbit IgG. Nature. 1967 Dec 30;216(5122):1295–1296. doi: 10.1038/2161295a0. [DOI] [PubMed] [Google Scholar]
  20. JOHNSON R. E., HALL A. P. Rubella arthritis; report of cases studied by latex tests. N Engl J Med. 1958 Apr 10;258(15):743–745. doi: 10.1056/NEJM195804102581506. [DOI] [PubMed] [Google Scholar]
  21. KUNKEL H. G., SIMON H. J., FUDENBERG H. Observations concerning positive serologic reactions for rheumatoid factor in certain patients with sarcoidosis and other hyperglobulinemic states. Arthritis Rheum. 1958 Aug;1(4):289–296. doi: 10.1002/art.1780010402. [DOI] [PubMed] [Google Scholar]
  22. LAWRENCE J. S., LAINE V. A., de GRAAFF The epidemiology of rheumatoid arthritis in Northern Europe. Proc R Soc Med. 1961 Jun;54:454–462. doi: 10.1177/003591576105400604. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Langenhuysen M. M. Antibodies against -globulin after blood transfusion and cytomegalovirus-infection. Clin Exp Immunol. 1971 Sep;9(3):393–398. [PMC free article] [PubMed] [Google Scholar]
  24. Markenson J. A., Daniels C. A., Notkins A. L., Hoofnagle J. H., Gerety J., Barker L. F. The interaction of rheumatoid factor with hepatitis B surface antigen-antibody complexes. Clin Exp Immunol. 1975 Feb;19(2):209–217. [PMC free article] [PubMed] [Google Scholar]
  25. Ohanian S. H., Borsos T. Lysis of tumor cells by antibody and complement. II. Lack of correlation between amount of C4 and C3 fixed and cell lysis. J Immunol. 1975 Apr;114(4):1292–1295. [PubMed] [Google Scholar]
  26. Panush R. S., Bianco N. E., Schur P. H. Serum and synovial fluid IgG, IgA and IgM antigammaglobulins in rheumatoid arthritis. Arthritis Rheum. 1971 Nov-Dec;14(6):737–747. doi: 10.1002/art.1780140609. [DOI] [PubMed] [Google Scholar]
  27. SCHROHENLOHER R. E., KUNKEL H. G., TOMASI T. B. ACTIVITY OF DISSOCIATED AND REASSOCIATED 19S ANTI-GAMMA-GLOBULINS. J Exp Med. 1964 Dec 1;120:1215–1229. doi: 10.1084/jem.120.6.1215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. SINGER J. M., PLOTZ C. M., PERLATA F. M., LYONS H. C. The presence of anti-gamma globulin factors in sera of patients with active pulmonary tuberculosis. Ann Intern Med. 1962 Apr;56:545–552. doi: 10.7326/0003-4819-56-4-545. [DOI] [PubMed] [Google Scholar]
  29. SINGER J. M. The latex fixation test in rheumatic diseases: a review. Am J Med. 1961 Nov;31:766–779. doi: 10.1016/0002-9343(61)90161-9. [DOI] [PubMed] [Google Scholar]
  30. SVEC K. H., DINGLE J. H. THE OCCURENCE OF RHEUMATOID FACTOR IN ASSOCIATION WITH ANTIBODY RESPONSE TO INFLUENZA A2(ASIAN) VIRUS. Arthritis Rheum. 1965 Aug;8:524–529. doi: 10.1002/art.1780080406. [DOI] [PubMed] [Google Scholar]
  31. Schmid F. R., Roitt I. M., Rocha M. J. Complement fixation by a two-component antibody system: immunoglobulin G and immunoglobulin M anti-globulin (rheumatoid factor). Parodoxical effect related to immunoglobulin G concentration. J Exp Med. 1970 Oct 1;132(4):673–693. doi: 10.1084/jem.132.4.673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Torrigiani G., Roitt I. M. Antiglobulin factors in sera from patients with rheumatoid arthritis and normal subjects. Quantitative estimation in different immunoglobulin classes. Ann Rheum Dis. 1967 Jul;26(4):334–340. doi: 10.1136/ard.26.4.334. [DOI] [PMC free article] [PubMed] [Google Scholar]

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