Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1977 Mar;15(3):745–750. doi: 10.1128/iai.15.3.745-750.1977

Antibacterial product of peritoneal exudate cell cultures from guinea pigs infected with mycobacteria, listeriae, and rickettsiae.

S D Sharma, G Middlebrook
PMCID: PMC421433  PMID: 15946

Abstract

In an in vitro model of cellular immunity, the antibacterial product of immunologically mediated mononuclear cell activation was studied from guinea pigs infected with listeriae and rickettsiae and compared with the product previously described from animals infected with mycobacteria. We found that this product, active against gram-positive bacilli, appeared to be identical in the three different infections with regard to its heat stability, its chromatographic adsorption and elution pattern, its susceptibility to inactivation by proteolytic enzymes, and its antibacterial spectrum

Full text

PDF
745

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bast R. C., Jr, Cleveland R. P., Littman B. H., Zbar B., Rapp H. J. Acquired cellular immunity: extracellular killing of Listeria monocytogenes by a product of immunologically activated macrophages. Cell Immunol. 1974 Feb;10(2):248–259. doi: 10.1016/0008-8749(74)90116-6. [DOI] [PubMed] [Google Scholar]
  2. Behin R., Mauel J., Biroum-Noerjasin, Rowe D. S. Mechanisms of protective immunity in experimental cutaneous leishmaniasis of the guinea-pig. II. Selective destruction of different Leishmania species in activated guinea-pig and mouse macrophages. Clin Exp Immunol. 1975 May;20(2):351–358. [PMC free article] [PubMed] [Google Scholar]
  3. Blanden R. V., Mackaness G. B., Collins F. M. Mechanisms of acquired resistance in mouse typhoid. J Exp Med. 1966 Oct 1;124(4):585–600. doi: 10.1084/jem.124.4.585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. David J. R. Lymphocyte mediators and cellular hypersensitivity. N Engl J Med. 1973 Jan 18;288(3):143–149. doi: 10.1056/NEJM197301182880311. [DOI] [PubMed] [Google Scholar]
  5. David J. R. Macrophage activation by lymphocyte mediators. Fed Proc. 1975 Jul;34(8):1730–1736. [PubMed] [Google Scholar]
  6. ELBERG S. S., SCHNEIDER P., FONG J. Cross-immunity between Brucella melitensis and Mycobacterium tuberculosis; intracellular behavior of Brucella melitensis in monocytes from vaccinated animals. J Exp Med. 1957 Oct 1;106(4):545–554. doi: 10.1084/jem.106.4.545. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fowles R. E., Fajardo I. M., Leibowitch J. L., David J. R. The enhancement of macrophage bacteriostasis by products of activated lymphocytes. J Exp Med. 1973 Oct 1;138(4):952–964. doi: 10.1084/jem.138.4.952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gambrill M. R., Wisseman C. L., Jr Mechanisms of immunity in typhus infections. I. Multiplication of typhus rickettsiae in human macrophage cell cultures in the nonimmune system: influence of virulence of rickettsial strains and of chloramphenicol. Infect Immun. 1973 Oct;8(4):519–527. doi: 10.1128/iai.8.4.519-527.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. HOLLAND J. J., PICKETT M. J. A cellular basis of immunity in experimental Brucella infection. J Exp Med. 1958 Sep 1;108(3):343–360. doi: 10.1084/jem.108.3.343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Koster F. T., McGregor D. D. The mediator of cellular immunity. 3. Lymphocyte traffic from the blood into the inflamed peritoneal cavity. J Exp Med. 1971 Apr 1;133(4):864–876. doi: 10.1084/jem.133.4.864. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. MACKANESS G. B. Cellular resistance to infection. J Exp Med. 1962 Sep 1;116:381–406. doi: 10.1084/jem.116.3.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. MACKANESS G. B. THE IMMUNOLOGICAL BASIS OF ACQUIRED CELLULAR RESISTANCE. J Exp Med. 1964 Jul 1;120:105–120. doi: 10.1084/jem.120.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mackaness G. B. The influence of immunologically committed lymphoid cells on macrophage activity in vivo. J Exp Med. 1969 May 1;129(5):973–992. doi: 10.1084/jem.129.5.973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. McGregor D. D., Koster F. T., Mackaness G. B. The mediator of cellular immunity. I. The life-span and circulation dynamics of the immunologically committed lymphocyte. J Exp Med. 1971 Feb 1;133(2):389–399. doi: 10.1084/jem.133.2.389. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. McGregor D. D., Logie P. S. The mediator of cellular immunity. VI. Effect of the antimitotic drug vinblastine on the mediator of cellular resistance to infection. J Exp Med. 1973 Mar 1;137(3):660–674. doi: 10.1084/jem.137.3.660. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Middlebrook G., Salmon B. J., Kreisberg J. I. Sterilization of Listeria monocytogenes by guinea pig peritoneal exudate cell cultures. Cell Immunol. 1974 Nov;14(2):270–283. doi: 10.1016/0008-8749(74)90211-1. [DOI] [PubMed] [Google Scholar]
  18. North R. J., Spitalny G. Inflammatory lymphocyte in cell-mediated antibacterial immunity: factors governing the accumulation of mediator T cells in peritoneal exudates. Infect Immun. 1974 Sep;10(3):489–498. doi: 10.1128/iai.10.3.489-498.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sethi K. K., Teschner M., Brandis H. In vitro antilisterial activity of soluble product(s) released from Listeria-immune murine peritoneal macrophages. Infect Immun. 1974 Oct;10(4):960–962. doi: 10.1128/iai.10.4.960-962.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sharma S. D., Middlebrook G. Antibacterial activity of stimulated guinea pig peritoneal exudate cell culture supernates. Cell Immunol. 1976 Jun 1;24(1):123–131. doi: 10.1016/0008-8749(76)90138-6. [DOI] [PubMed] [Google Scholar]
  21. Simon H. B., Sheagren J. N. Cellular immunity in vitro. I. Immunologically mediated enhancement of macrophage bactericidal capacity. J Exp Med. 1971 Jun 1;133(6):1377–1389. doi: 10.1084/jem.133.6.1377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Simon H. B., Sheagren J. N. Enhancement of macrophage bactericidal capacity by antigenically stimulated immune lymphocytes. Cell Immunol. 1972 Jun;4(2):163–174. doi: 10.1016/0008-8749(72)90015-9. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES