Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1977 Apr;16(1):107–109. doi: 10.1128/iai.16.1.107-109.1977

Oral toxicities of Clostridium botulinum toxins in response to molecular size.

I Ohishi, S Sugii, G Sakaguchi
PMCID: PMC421495  PMID: 326664

Abstract

Clostridium botulinum type A, B, and F toxins of different molecular sizes were fed to mice to compare the oral toxicities. The progenitor toxin, a complex of a toxic and nontoxic component, of any type was higher in oral toxicity to mice than the dissociated toxic component or the derivative toxin. The former may no doubt play a more important role in the pathogenesis of food-borne botulism. The higher oral toxicity possessed by the progenitor toxin, including the exceptionally high one found with type B-L toxin, can be explained solely by the protection afforded by the nontoxic component attached to the toxic component. The possibility of the highest oral toxicity of type B-L toxin to humans is discussed.

Full text

PDF
107

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DOLMAN C. E., DARBY G. E., LANE R. F. Type E botulism due to salmon eggs. Can J Public Health. 1955 Apr;46(4):135–141. [PubMed] [Google Scholar]
  2. DUFF J. T., WRIGHT G. G., KLERER J., MOORE D. E., BIBLER R. H. Studies on immunity to toxins of Clostridium botulinum. I. A simplified procedure for isolation of type A toxin. J Bacteriol. 1957 Jan;73(1):42–47. doi: 10.1128/jb.73.1.42-47.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DUFF J. T., WRIGHT G. G., YARINSKY A. Activation of Clostridium botulinum type E toxin by trypsin. J Bacteriol. 1956 Oct;72(4):455–460. doi: 10.1128/jb.72.4.455-460.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kitamura M., Sakaguchi G. Dissociation and reconstitution of 12-S toxin of Clostridium botulinum type E. Biochim Biophys Acta. 1969 Dec 23;194(2):564–571. doi: 10.1016/0005-2795(69)90118-4. [DOI] [PubMed] [Google Scholar]
  5. Kitamura M., Sakaguchi S., Sakaguchi G. Significance of 12S toxin of Clostridium botulinum type E. J Bacteriol. 1969 Jun;98(3):1173–1178. doi: 10.1128/jb.98.3.1173-1178.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kozaki S., Sakaguchi S., Sakaguchi G. Purification and some properties of progenitor toxins of Clostridium botulinum type B. Infect Immun. 1974 Oct;10(4):750–756. doi: 10.1128/iai.10.4.750-756.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lamanna C., Sakaguchi G. Botulinal toxins and the problem of nomenclature of simple toxins. Bacteriol Rev. 1971 Sep;35(3):242–249. doi: 10.1128/br.35.3.242-249.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. MEYER K. F., EDDIE B. Perspectives concerning botulism. Z Hyg Infektionskr. 1951;133(4):255–263. doi: 10.1007/BF02149050. [DOI] [PubMed] [Google Scholar]
  9. Ohishi I., Sakaguchi G. Molecular construction of Clostridium botulinum type F progenitor toxin. Appl Microbiol. 1975 Apr;29(4):444–447. doi: 10.1128/am.29.4.444-447.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Oishi I., Sakaguchi G. Purification of Clostridium botuliunum type F progenitor toxin. Appl Microbiol. 1974 Dec;28(6):923–928. doi: 10.1128/am.28.6.923-928.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sakaguchi G., Sakaguchi S., Karashimada T. Molecular size of Clostridium botulinum type E toxin in "izushi". Jpn J Med Sci Biol. 1966 Aug;19(4):201–207. [PubMed] [Google Scholar]
  12. Sakaguchi G., Sakaguchi S., Kondo H. Rapid bioassay for Clostridium botulinum type-E toxins by intravenous injection into mice. Jpn J Med Sci Biol. 1968 Dec;21(6):369–378. doi: 10.7883/yoken1952.21.369. [DOI] [PubMed] [Google Scholar]
  13. Sakaguchi G., Sakaguchi S. Oral toxicities of Clostridium botulinum type E toxins of different forms. Jpn J Med Sci Biol. 1974 Aug;27(4):241–244. doi: 10.7883/yoken1952.27.241. [DOI] [PubMed] [Google Scholar]
  14. Sugii S., Sakaguchi G. Molecular construction of Clostridium botulinum type A toxins. Infect Immun. 1975 Dec;12(6):1262–1270. doi: 10.1128/iai.12.6.1262-1270.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sugiyama H., Das Gupta R., Yang K. H. Disulfide-toxicity relationship of botulinal toxin types A, E, and F. Proc Soc Exp Biol Med. 1973 Jul;143(3):589–591. doi: 10.3181/00379727-143-37372. [DOI] [PubMed] [Google Scholar]
  16. Sugiyama H., DasGupta B. R., Yang K. H. Toxicity of purified botulinal toxin fed to mice. Proc Soc Exp Biol Med. 1974 Nov;147(2):589–591. doi: 10.3181/00379727-147-38394. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES