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. 1977 Apr;16(1):181–188. doi: 10.1128/iai.16.1.181-188.1977

Serial enzymatic hydrolysis of cell walls of two serotypes of yeast-form Histoplasma capsulatum with alpha(1 leads to 3)-glucanase, beta(1 leads to 3)-glucanase, pronase, and chitinase.

E Reiss
PMCID: PMC421506  PMID: 873605

Abstract

A serial enzymatic hydrolysis procedure for the partial lysis of Histoplasma capsulatum yeast-form cell walls was described, and its application for the differentiation of two serotypes was evaluated. Cell walls were serially digested with alpha(1 leads to 3)-glucanase and beta(1 leads to 3)-glucanse of Cladosporium resinae, then by Pronase, and then by chitinase. The walls of serotype 1, 2, 3 (61.5% digested) were not susceptible to alpha(1 leads to 3)-glucanse, and they contained 30.3% chitin, thus identifying the strain as chemotype 1 (chem 1). Serotype 1, 4 cells walls (51.6% digested) released 27.3% as glucose after treatment with alpha(1 leads to 3)-glucanse and contained 7.8% chitin, compatible with chemotype 2 (chem 2). In addition to quantitating the monomeric products of enzymolysis, I recovered soluble nondialyzable polysaccharide from the digests of both serotypes.

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Selected References

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  1. Bartnicki-Garcia S. Cell wall chemistry, morphogenesis, and taxonomy of fungi. Annu Rev Microbiol. 1968;22:87–108. doi: 10.1146/annurev.mi.22.100168.000511. [DOI] [PubMed] [Google Scholar]
  2. Bradley G., Pine L., Reeves M. W., Moss C. W. Purification, composition, and serological characterization of histoplasmin-H and M antigens. Infect Immun. 1974 May;9(5):870–880. doi: 10.1128/iai.9.5.870-880.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cabib E., Bowers B. Chitin and yeast budding. Localization of chitin in yeast bud scars. J Biol Chem. 1971 Jan 10;246(1):152–159. [PubMed] [Google Scholar]
  4. Chattaway F. W., Holmes M. R., Barlow A. J. Cell wall composition of the mycelial and blastospore forms of Candida albicans. J Gen Microbiol. 1968 May;51(3):367–376. doi: 10.1099/00221287-51-3-367. [DOI] [PubMed] [Google Scholar]
  5. Colonna W. J., Lampen J. O. Structure of the mannan form Saccharomyces strain FH4C, a mutant constitutive for invertase biosynthesis. I. Significance of phosphate to the structure and refractoriness of the molecule. Biochemistry. 1974 Jun 18;13(13):2741–2748. doi: 10.1021/bi00710a013. [DOI] [PubMed] [Google Scholar]
  6. Domer J. E. Monosaccharide and chitin content of cell walls of Histoplasma capsulatum and Blastomyces dermatitidis. J Bacteriol. 1971 Sep;107(3):870–877. doi: 10.1128/jb.107.3.870-877.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ebisu S., Kato K., Kotani S., Misaki A. Isolation and purification of Flavobacterium alpha-1,3-glucanase-hydrolyzing, insoluble, sticky glucan of Streptococcus mutans. J Bacteriol. 1975 Dec;124(3):1489–1501. doi: 10.1128/jb.124.3.1489-1501.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Feit C., Tewari R. P. Immunogenicity of Ribosomal Preparations from Yeast Cells of Histoplasma capsulatum. Infect Immun. 1974 Nov;10(5):1091–1097. doi: 10.1128/iai.10.5.1091-1097.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fleet G. H., Phaff H. J. Glucanases in Schizosaccharomyces. Isolation and properties of the cell wall-associated beta(1 leads to 3)-glucanases. J Biol Chem. 1974 Mar 25;249(6):1717–1728. [PubMed] [Google Scholar]
  10. Garcia J. P., Howard D. H. Characterization of antigens from the yeast phase of Histoplasma capsulatum. Infect Immun. 1971 Aug;4(2):116–125. doi: 10.1128/iai.4.2.116-125.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HEINER D. C. Diagnosis of histoplasmosis using precipitin reactions in agargel. Pediatrics. 1958 Oct;22(4 Pt 1):616–627. [PubMed] [Google Scholar]
  12. Hasegawa S., Nordin J. H. Enzymes that hydrolyze fungal cell wall polysaccharides. I. Purification and properties of an endo-alpha-D-(1-3)-glucanase from Trichoderma. J Biol Chem. 1969 Oct 25;244(20):5460–5470. [PubMed] [Google Scholar]
  13. KAUFMAN L., KAPLAN W. Preparation of a fluorescent antibody specific for the yeast phase of Histoplasma capsulatum. J Bacteriol. 1961 Nov;82:729–735. doi: 10.1128/jb.82.5.729-735.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. KESSLER G., NICKERSON W. J. Glucomannan-protein complexes from cell walls of yeasts. J Biol Chem. 1959 Sep;234:2281–2285. [PubMed] [Google Scholar]
  15. Kanetsuna F., Carbonell L. M., Gil F., Azuma I. Chemical and ultrastructural studies on the cell walls of the yeastlike and mycelial forms of Histoplasma capsulatum. Mycopathol Mycol Appl. 1974 Oct 15;54(1):1–13. doi: 10.1007/BF02055967. [DOI] [PubMed] [Google Scholar]
  16. Kaufman L., Blumer S. Occurrence of serotypes among Histoplasma capsulatum strains. J Bacteriol. 1966 Apr;91(4):1434–1439. doi: 10.1128/jb.91.4.1434-1439.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kopecká M., Phaff H. J., Fleet G. H. Demonstration of a fibrillar component in the cell wall of the yeast Saccharomyces cerevisiae and its chemical nature. J Cell Biol. 1974 Jul;62(1):66–76. doi: 10.1083/jcb.62.1.66. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. MANDELS M., PARRISH F. W., REESE E. T. Sophorose as an inducer of cellulase in Trichoderma viride. J Bacteriol. 1962 Feb;83:400–408. doi: 10.1128/jb.83.2.400-408.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Mann J. W., Heintz C. E., Macmillan J. D. Yeast spheroplasts formed by cell wall-degrading enzymes from Oerskovia sp. J Bacteriol. 1972 Sep;111(3):821–824. doi: 10.1128/jb.111.3.821-824.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Manners D. J., Masson A. J., Patterson J. C. The structure of a beta-(1 leads to 3)-D-glucan from yeast cell walls. Biochem J. 1973 Sep;135(1):19–30. doi: 10.1042/bj1350019. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Marriott M. S. Isolation and chemical characterization of plasma membranes from the yeast and mycelial forms of Candida albicans. J Gen Microbiol. 1975 Jan;86(1):115–132. doi: 10.1099/00221287-86-1-115. [DOI] [PubMed] [Google Scholar]
  22. Monreal J., Reese D. T. Lysis of living candida utilis cells by enzymes of micromonospora. Arch Biochem Biophys. 1968 Sep 10;126(3):960–962. doi: 10.1016/0003-9861(68)90492-x. [DOI] [PubMed] [Google Scholar]
  23. Monreal J., Reese E. T. The chitinase of Serratia marcescens. Can J Microbiol. 1969 Jul;15(7):689–696. doi: 10.1139/m69-122. [DOI] [PubMed] [Google Scholar]
  24. Odds F. C., Kaufman L., McLaughlin D., Callaway C., Blumer S. O. Effect of chitinase complex on the antigenicity and chemistry of yeast-form cell walls and other fractions of Histoplasma capsulatum and Blastomyces dermatitidis. Sabouraudia. 1974 Jul;12(2):138–149. doi: 10.1080/00362177485380201. [DOI] [PubMed] [Google Scholar]
  25. Pine L., Boone C. J. Cell wall composition and serological reactivity of Histoplasma capsulatum serotypes and related species. J Bacteriol. 1968 Sep;96(3):789–798. doi: 10.1128/jb.96.3.789-798.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Reiss E., Mitchell W. O., Stone S. H., Hasenclever H. F. Cellular immune activity of a galactomannan-protein complex from mycelia of Histoplasma capsulatum. Infect Immun. 1974 Oct;10(4):802–809. doi: 10.1128/iai.10.4.802-809.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Reiss E., Stone S. H., Hasenclever H. F. Serological and cellular immune activity of peptidoglucomannan fractions of Candida albicans cell walls. Infect Immun. 1974 May;9(5):881–890. doi: 10.1128/iai.9.5.881-890.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. San-Blas G., Carbonell L. M. Chemical and ultrastructural studies on the cell walls of the yeastlike and mycelial forms of Histoplasma farciminosum. J Bacteriol. 1974 Aug;119(2):602–611. doi: 10.1128/jb.119.2.602-611.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Sentandreu R., Northcote D. H. The structure of a glycopeptide isolated from the yeast cell wall. Biochem J. 1968 Sep;109(3):419–432. doi: 10.1042/bj1090419. [DOI] [PMC free article] [PubMed] [Google Scholar]

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