Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1978 Apr;20(1):130–135. doi: 10.1128/iai.20.1.130-135.1978

Orofacial Herpes Simplex Virus Infection in Hairless Mice: Latent Virus in Trigeminal Ganglia After Topical Antiviral Treatment †

Richard J Klein 1, Alvin E Friedman-Kien 1, Paul B Yellin 1
PMCID: PMC421562  PMID: 208970

Abstract

Inoculation of herpes simplex virus on the forehead and/or snout of hairless mice resulted in a significantly lower mortality rate than inoculation of the skin in the lumbosacral area. Latent herpes simplex virus infections were detected in all forehead-inoculated and in 90% of snout-inoculated mice. Phosphonoacetic acid was highly effective in preventing the development of skin lesions, and no latent infections were detected when phosphonoacetic acid ointment was applied 3 h after infection. Neither adenine arabinoside nor adenine arabinoside monophosphate prevented the establishment of latent infections in the trigeminal ganglia, although they protected the mice from the fatal outcome of the infection. The antibody response after adenine arabinoside or adenine arabinoside monophosphate treatment was similar to that observed in untreated animals, and it was six to eight times higher than in mice treated with phosphonoacetic acid. Mice without evidence of latent infection had, in general, lower serum antibody titers than those with latent infections in the ganglia. An analysis of the pathogenesis of herpes simplex virus infection in mice treated with adenine arabinoside showed that virus penetration into the nerve endings was delayed and that the amount of free virus in ganglionic homogenates was 10 to 100 times less than that for untreated mice.

Full text

PDF
130

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cook M. L., Stevens J. G. Latent herpetic infections following experimental viraemia. J Gen Virol. 1976 Apr;31(1):75–80. doi: 10.1099/0022-1317-31-1-75. [DOI] [PubMed] [Google Scholar]
  2. Hill T. J., Field H. J., Blyth W. A. Acute and recurrent infection with herpes simplex virus in the mouse: a model for studying latency and recurrent disease. J Gen Virol. 1975 Sep;28(3):341–353. doi: 10.1099/0022-1317-28-3-341. [DOI] [PubMed] [Google Scholar]
  3. Kitces E. N., Morahan P. S., Tew J. G., Murray B. K. Protection from oral herpes simplex virus infection by a nucleic acid-free virus vaccine. Infect Immun. 1977 Jun;16(3):955–960. doi: 10.1128/iai.16.3.955-960.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Klein R. J., Friedman-Kien A. E., Fondak A. A., Buimovici-Klein E. Immune response and latent infection after topical treatment of herpes simplex virus infection in hairless mice. Infect Immun. 1977 Jun;16(3):842–848. doi: 10.1128/iai.16.3.842-848.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Klein R. J., Friedman-Kien A. E. Latent herpes simplex virus infections in sensory ganglia of mice after topical treatment with adenine arabinoside and adenine arabinoside monophosphate. Antimicrob Agents Chemother. 1977 Nov;12(5):577–581. doi: 10.1128/aac.12.5.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Knotts F. B., Cook M. L., Stevens J. G. Latent herpes simplex virus in the central nervous system of rabbits and mice. J Exp Med. 1973 Sep 1;138(3):740–744. doi: 10.1084/jem.138.3.740. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LORNEZ R. J. [On statistics of the plaque test]. Arch Gesamte Virusforsch. 1962;12:108–137. [PubMed] [Google Scholar]
  8. Price R. W., Katz B. J., Notkins A. L. Latent infection of the peripheral ANS with herpes simplex virus. Nature. 1975 Oct 23;257(5528):686–688. doi: 10.1038/257686a0. [DOI] [PubMed] [Google Scholar]
  9. Price R. W., Walz M. A., Wohlenberg C., Notkins A. L. Latent infection of sensory ganglia with herpes simplex virus: efficacy of immunization. Science. 1975 May 30;188(4191):938–940. doi: 10.1126/science.166432. [DOI] [PubMed] [Google Scholar]
  10. Stevens J. G., Cook M. L. Latent herpes simplex virus in spinal ganglia of mice. Science. 1971 Aug 27;173(3999):843–845. doi: 10.1126/science.173.3999.843. [DOI] [PubMed] [Google Scholar]
  11. Walz M. A., Price R. W., Notkins A. L. Latent ganglionic infection with herpes simplex virus types 1 and 2: viral reactivation in vivo after neurectomy. Science. 1974 Jun 14;184(4142):1185–1187. doi: 10.1126/science.184.4142.1185. [DOI] [PubMed] [Google Scholar]
  12. Walz M. A., Yamamoto H., Notkins A. L. Immunological response restricts number of cells in sensory ganglia infected with herpes simplex virus. Nature. 1976 Dec 9;264(5586):554–556. doi: 10.1038/264554a0. [DOI] [PubMed] [Google Scholar]
  13. Wohlenberg C. R., Walz M. A., Notkins A. L. Efficacy of phosphonoacetic acid on herpes simplex virus infection of sensory ganglia. Infect Immun. 1976 May;13(5):1519–1521. doi: 10.1128/iai.13.5.1519-1521.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES