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. 1978 Apr;20(1):167–172. doi: 10.1128/iai.20.1.167-172.1978

Isolation of the L-phase variant from toxigenic Corynebacterium diphtheriae C7(beta).

C Kanel, T Uchida, M Yoneda
PMCID: PMC421568  PMID: 97222

Abstract

The L-phase variant was isolated from lysogenic strain C7(beta) of Corynebacterium diphtheriae by the penicillin disk tecnnique on an agar medium containing 3.7% brain heart infusion, 3% NaCl, 1.25% Grycine, 1% Noble agar, and 10% horse serum. The L-phase formed a "fried egg" colony; it was resistant to antibiotics that inhibit biosynthesis of bacterial cell walls but was susceptible to antibiotics that act on the protein-synthesizing system. After several transfers of the L-phase initially isolated, the L-phase of strain C7(beta) became stable, forming a typical L-phase colony on the agar even without penicillin. The L-phase variant retained lysogenicity and toxigenicity. The toxin production by this L-phase variant was, like that by the bacterial phase, inhibited by excess iron.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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