Table 1.
Gene | Protein name | vs. RLL
|
vs. Elast
|
vs. Oldlg
|
IPI | |||
---|---|---|---|---|---|---|---|---|
Old lung | Elast | Old elast | Bleo | Old elast | Old elast | |||
Cytoskeletal | ||||||||
Actn4 | Alpha-actinin-4 | 0.200 | 1.000 | 0.470 | 0.048 | 0.643 | 0.467 | IPI00118899.1 |
Cbr2 | Carbonyl reductase [NADPH] 2 | 0.200 | 0.821 | 0.320 | 0.905 | 0.952 | 0.043 | IPI00128642.1 |
Flna | Isoform 1 of Filamin-A | 0.086 | 0.107 | 0.002 | 0.310 | 0.024 | 0.005 | IPI00131138.10 |
Plec | Plectin isoform 1b2alpha | 0.200 | 0.833 | 0.106 | 0.750 | 0.024 | 0.005 | IPI00229509.3 |
Spna2 | Isoform 2 of Spectrin alpha chain, brain | 0.810 | 0.833 | 0.011 | 0.036 | 0.012 | 0.014 | IPI00753793.2 |
Tuba1a | Tubulin alpha-1A chain | 0.005 | 0.119 | 0.087 | 0.012 | 0.714 | 0.010 | IPI00110753.1 |
Tuba1b | Tubulin alpha-1B chain | 1.000 | 1.000 | 0.061 | 0.012 | 0.155 | 0.133 | IPI00117348.4 |
Tubb2b | Tubulin beta-2B chain | 0.910 | 0.571 | 0.022 | 0.119 | 0.071 | 0.010 | IPI00109061.1 |
Vcl | Vinculin | 0.952 | 0.357 | 0.106 | 0.214 | 1.000 | 0.010 | IPI00405227.3 |
Vim | Vimentin | 0.048 | 0.714 | 0.158 | 0.381 | 0.012 | 0.005 | IPI00227299.6 |
Extracellular matrix proteins | ||||||||
Col1a2 | Collagen alpha-2(I) chain | 0.281 | 0.048 | 0.002 | 1.000 | 0.012 | 0.067 | IPI00222188.4 |
Col4a2 | Collagen alpha-2(IV) chain | 0.019 | 0.012 | 0.013 | 0.012 | 0.762 | 0.524 | IPI00338452.3 |
Col6a1 | Collagen alpha-1(VI) chain | 0.005 | 0.274 | 0.013 | 0.024 | 0.036 | 0.381 | IPI00339885.2 |
Col6a2 | Collagen alpha-2(VI) chain | 0.938 | 0.155 | 0.485 | 0.036 | 0.393 | 0.829 | IPI00621027.2 |
Col6a3 | collagen alpha-3(VI) chain | 0.133 | 0.024 | 0.004 | 0.702 | 0.238 | 0.019 | IPI00830749.3 |
Eln | Elastin | 0.619 | 0.548 | 0.632 | 0.024 | 0.738 | 0.381 | IPI00134505.1 |
Emilin1 | EMILIN-1 | 0.057 | 0.012 | 0.002 | 0.012 | 1.000 | 0.033 | IPI00115516.1 |
Fbln5 | Fibulin-5 | 0.033 | 0.083 | 0.028 | 0.929 | 1.000 | 1.000 | IPI00323035.3 |
Fbn1_1 | Fibrillin-1 | 1.000 | 0.083 | 1.000 | 0.012 | 0.083 | 1.000 | IPI00122438.1 |
Fga | Fibrinogen, alpha polypeptide isoform 2 | 0.467 | 0.750 | 0.455 | 0.012 | 0.333 | 1.000 | IPI00115522.3 |
Fgb | Fibrinogen beta chain | 0.957 | 0.810 | 0.182 | 0.012 | 0.333 | 0.033 | IPI00279079.1 |
Fgg | Putative uncharacterized protein | 0.043 | 0.202 | 0.015 | 0.012 | 0.333 | 1.000 | IPI00122312.2 |
Fn1_1 | Fibronectin | 0.871 | 0.024 | 0.303 | 0.012 | 0.202 | 0.400 | IPI00113539.2 |
Hspg2_1 | Basement membrane-specific heparan sulfate proteoglycan core protein | 0.005 | 0.333 | 0.275 | 0.262 | 1.000 | 0.043 | IPI00113824.1 |
Hspg2_2 | Basement membrane-specific heparan sulfate proteoglycan core protein | 0.033 | 1.000 | 0.028 | 0.881 | 0.012 | 1.000 | IPI00515360.8 |
Lama4 | Laminin subunit alpha-4 | 0.014 | 0.036 | 0.002 | 0.024 | 0.333 | 0.400 | IPI00223446.5 |
Lama5 | Laminin subunit alpha-5 | 0.329 | 0.643 | 0.693 | 0.321 | 0.917 | 0.200 | IPI00116913.3 |
Lamb1 | Laminin subunit beta-1 | 0.010 | 0.012 | 0.002 | 0.060 | 0.012 | 0.005 | IPI00338785.3 |
Lamb2 | Laminin subunit beta-2 precursor | 0.029 | 0.393 | 0.004 | 0.321 | 0.119 | 0.114 | IPI00109612.2 |
Lamb3 | Laminin subunit beta-3 | 0.243 | 0.214 | 0.006 | 0.060 | 0.071 | 0.048 | IPI00117093.1 |
Lamc1 | Laminin subunit gamma-1 | 0.143 | 0.893 | 0.045 | 0.333 | 0.071 | 0.310 | IPI00400016.1 |
Lamc2 | Laminin subunit gamma-2 | 0.005 | 0.012 | 0.004 | 0.155 | 0.488 | 0.857 | IPI00117115.3 |
Myh10 | Myosin, heavy polypeptide 10, non-muscle | 0.038 | 0.107 | 0.002 | 0.048 | 0.012 | 0.005 | IPI00338604.5 |
Myh11_1 | Isoform 1 of Myosin-11 | 0.357 | 0.571 | 0.002 | 0.298 | 0.012 | 0.014 | IPI00114894.1 |
Myh14 | Isoform 1 of Myosin-14 | 0.567 | 0.060 | 0.004 | 0.167 | 0.012 | 0.005 | IPI00453996.1 |
Myh6 | Myosin-6 | 0.162 | 0.048 | 0.095 | 0.095 | 0.012 | 0.005 | IPI00129404.1 |
Myh9 | Myosin-9 | 0.562 | 0.155 | 0.002 | 0.012 | 0.012 | 0.005 | IPI00123181.4 |
Nid1 | Nidogen-1 | 0.019 | 0.167 | 0.022 | 1.000 | 0.345 | 0.467 | IPI00111793.1 |
Intracellular cytoplasmic | ||||||||
Aco2 | Aconitate hydratase, mitochondrial | 0.033 | 0.333 | 1.000 | 1.000 | 0.333 | 0.033 | IPI00116074.1 |
Actb | Actin, cytoplasmic 1 | 0.076 | 0.155 | 0.227 | 0.071 | 0.024 | 0.500 | IPI00110850.1 |
Ctnna1 | Catenin alpha-1 | 0.900 | 0.536 | 0.455 | 0.202 | 0.012 | 0.400 | IPI00112963.1 |
Des | Desmin | 0.729 | 0.012 | 0.006 | 0.060 | 0.583 | 0.010 | IPI00130102.4 |
Dpysl2 | Dihydropyrimidinase-related protein 2 | 1.000 | 0.631 | 0.132 | 0.512 | 0.048 | 0.152 | IPI00114375.2 |
Eef2 | Elongation factor 2 | 0.010 | 0.940 | 0.697 | 0.012 | 0.750 | 0.010 | IPI00466069.3 |
Hadha | Trifunctional enzyme subunit alpha, mitochondrial | 0.010 | 0.012 | 1.000 | 0.012 | 0.012 | 0.010 | IPI00223092.5 |
Hadhb | Trifunctional enzyme subunit beta, mitochondrial | 0.400 | 1.000 | 1.000 | 1.000 | 1.000 | 0.400 | IPI00115607.3 |
Hbaa1 | Putative uncharacterized protein | 1.000 | 0.440 | 0.182 | 1.000 | 0.012 | 0.033 | IPI00110658.1 |
Hbbb1 | LOC100503164 Beta-globin | 0.867 | 0.393 | 0.069 | 0.095 | 0.036 | 0.095 | IPI00762198.2 |
Myo1c | Isoform 2 of Myosin-Ic | 0.019 | 1.000 | 0.673 | 0.750 | 0.893 | 0.143 | IPI00467172.2 |
Serpinh1 | Serpin H1 | 1.000 | 1.000 | 1.000 | 0.012 | 1.000 | 1.000 | IPI00114733.1 |
Tgm2 | Protein-glutamine gamma-glutamyltransferase 2 | 0.005 | 0.024 | 0.141 | 0.012 | 0.202 | 0.010 | IPI00126861.3 |
Ywhaz | 14-3-3 protein zeta/delta | 0.033 | 1.000 | 1.000 | 1.000 | 1.000 | 0.033 | IPI00116498.1 |
Intracellular nuclear | ||||||||
Eef1a2 | Elongation factor 1-alpha 2 | 1.000 | 0.333 | 1.000 | 0.012 | 1.000 | 1.000 | IPI00119667.1 |
Hist1h2bf_j_l_n | Histone H4 | 0.005 | 0.631 | 0.004 | 0.607 | 0.012 | 0.005 | IPI00407339.7 |
Hist1h2bn_f_j_l | Histone Type 1-F/J/L. | 0.033 | 0.893 | 0.552 | 0.024 | 0.798 | 0.019 | IPI00114642.4 |
Hnrnpk | Isoform 2 of Heterogeneous nuclear ribonucleoprotein K | 0.033 | 0.083 | 0.035 | 0.083 | 0.821 | 0.200 | IPI00224575.1 |
Ptrf | Polymerase I and transcript release factor | 0.400 | 0.012 | 0.015 | 0.012 | 0.048 | 0.048 | IPI00117689.1 |
Membrane | ||||||||
Anxa1 | Annexin A1 | 1.000 | 0.750 | 1.000 | 0.024 | 0.750 | 1.000 | IPI00230395.5 |
Anxa2 | Annexin A2 | 1.000 | 0.083 | 1.000 | 0.012 | 0.083 | 1.000 | IPI00468203.3 |
Atp5a1 | ATP synthase subunit alpha, mitochondrial | 0.029 | 0.714 | 0.232 | 0.048 | 0.250 | 0.005 | IPI00130280.1 |
Atp5b | ATP synthase subunit beta, mitochondrial | 0.038 | 0.071 | 0.429 | 0.857 | 0.143 | 0.071 | IPI00468481.2 |
Ehd2 | Eh domain-containing protein 1 | 0.490 | 0.036 | 0.251 | 0.488 | 0.131 | 0.190 | IPI00126083.3 |
Iqgap1 | Ras GTPase-activating-like protein IQGAP1 | 0.052 | 0.833 | 0.009 | 0.024 | 0.202 | 0.714 | IPI00467447.3 |
Npnt | Isoform 4 of Nephronectin | 1.000 | 0.083 | 1.000 | 0.012 | 0.083 | 1.000 | IPI00124689.1 |
Samhd1 | Mimecan | 0.033 | 1.000 | 1.000 | 0.333 | 1.000 | 0.033 | IPI00120848.1 |
Slc25a4 | ADP/ATP translocase 1 | 1.000 | 0.333 | 0.455 | 0.012 | 0.643 | 0.467 | IPI00115564.5 |
Vdac2 | Voltage-dependent anion-selective channel protein 2 | 0.033 | 0.333 | 1.000 | 0.333 | 0.333 | 0.033 | IPI00122547.1 |
Tln1 | Talin-1 | 0.010 | 0.619 | 0.002 | 0.345 | 0.012 | 0.129 | IPI00465786.3 |
Values represent p-values from two group comparisons of raw unique peptide hits using the non-parametric test (pre-planned, selected, pairwise comparisons are shown in the top two rows). Protein group assignments were generated from UniProt entries of positively identified proteins (proteins containing two or more unique peptide hits). Grayed boxes with italicized text indicate the treatment group (labeled in the second row) reached statistical significance (p-values less than 0.05) with higher amounts of unique peptide hits than the control group. White boxes with black bolded text indicate the control group (labeled in the top row) contained significantly more unique peptide hits.
Italics – treatment group significantly higher.
Black – control significantly higher (designated above).
Key: Elas = Elastase; Bleo = Bleomycin; Old Elast = Old Elastase; Oldlg = Old Mouse; RLL = Young Mouse, Right Lower Lobe.