Abstract
A platelet migration inhibition test was devised to determine the presence of antiplatelet activity in serum collected from experimentally produced and natural cases of canine ehrlichiosis. The maximum platelet migration inhibition effect was observed during the acute phase of the disease and before the appearance of specific humoral antibody, measured by the indirect fluorescent-antibody test. Platelet migration inhibition may be one of the earliest events leading to pancytopenia. In most cases, sera positive for humoral antibodies also were positive for platelet migration inhibition, although no direct correlation was evident between the serological titer and the degree of platelet migration inhibition. Inoculation of dogs with uninfected canine blood did not induce the production of inhibition factor or antibody activity, which precluded a histocompatibility response to the cellular elements in the inoculum. Scanning electron microscopy indicated that the platelet inhibition factor interfered with platelet migration by inhibiting pseudopod formation. Affected platelets became rounded and showed evidence of clumping and leakage.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Buhles W. C., Jr, Huxsoll D. L., Ristic M. Tropical canine pancytopenia: Clinical, hematologic, and serologic response of dogs to Ehrlichia canis infection, tetracycline therapy, and challenge inoculation. J Infect Dis. 1974 Oct;130(4):357–367. doi: 10.1093/infdis/130.4.357. [DOI] [PubMed] [Google Scholar]
- Duquesnoy R. J., Lorentsen D. F., Aster R. H. Platelet migration inhibition: a new method for detection of platelet antibodies. Blood. 1975 Jun;45(6):741–747. [PubMed] [Google Scholar]
- Hand W. L., Miller J. B., Reinarz J. A., Sanford J. P. Rocky Mountain spotted fever. A vascular disease. Arch Intern Med. 1970 May;125(5):879–882. [PubMed] [Google Scholar]
- Ristic M., Huxsoll D. L., Weisiger R. M., Hildebrandt P. K., Nyindo M. B. Serological diagnosis of tropical canine pancytopenia by indirect immunofluorescence. Infect Immun. 1972 Sep;6(3):226–231. doi: 10.1128/iai.6.3.226-231.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith R. D., Ristic M., Huxsoll D. L., Baylor R. A. Platelet kinetics in canine ehrlichiosis: evidence for increased platelet destruction as the cause of thrombocytopenia. Infect Immun. 1975 Jun;11(6):1216–1221. doi: 10.1128/iai.11.6.1216-1221.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weisiger R. M., Ristic M., Huxsoll D. L. Kinetics of antibody response to Ehrlichia canis assayed by the indirect fluorescent antibody method. Am J Vet Res. 1975 May;36(5):689–694. [PubMed] [Google Scholar]