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. 1978 May;20(2):375–380. doi: 10.1128/iai.20.2.375-380.1978

Production and partial purification of Salmonella enterotoxin.

D M Sedlock, L R Koupal, R H Deibel
PMCID: PMC421865  PMID: 352941

Abstract

By using a strain of Salmonella typhimurium, we detected the presence of an enterotoxin, as determined by the rabbit ileal loop assay, in various complex and defined media. The enterotoxin was concentrated by ultrafiltration of culture supernatant fluids and eluted in and adjacent to the void volume of a Sephadex G-100 column. This suggested that the enterotoxic factor was of a relatively high molecular weight, and additional evidence indicated it was heterogeneous in size. Further chromatography, using a diethylaminoethyl-cellulose anion exchanger, facilitated at least a 50-fold purification of the Salmonella enterotoxin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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