Skip to main content
Infection and Immunity logoLink to Infection and Immunity
. 1978 May;20(2):418–420. doi: 10.1128/iai.20.2.418-420.1978

Cell envelope of Neisseria gonorrhoeae: phospholipase activity and its relationship to autolysis.

A F Cacciapuoti, W S Wegener, S A Morse
PMCID: PMC421872  PMID: 27458

Abstract

The relationship between conditions which permit or inhibit cell lysis and those which promote phospholipid hydrolysis in Neisseria gonorrhoeae was investigated. Suspension of exponential-phase gonococci in buffer in the absence of divalent cations resulted in autolysis but not in phosphlipid hydrolysis. The addition of Ca2+ or Mg2+ to the buffer inhibited autolysis and markedly stimulated the hydrolysis of phosphatidylethanolamine. Incubation of cells in buffer at pH 6 inhibited both autolysis and phospholipid hydrolysis.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albright F. R., White D. A., Lennarz W. J. Studies on enzymes involved in the catabolism of phospholipids in Escherichia coli. J Biol Chem. 1973 Jun 10;248(11):3968–3977. [PubMed] [Google Scholar]
  2. Beebe J. L., Wlodkowski T. J. Lipids of Branhamella catarrhalis and Neisseria gonorrhoeae. J Bacteriol. 1976 Jul;127(1):168–178. doi: 10.1128/jb.127.1.168-178.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Elmros T., Burman L. G., Bloom G. D. Autolysis of Neisseria gonorrhoeae. J Bacteriol. 1976 May;126(2):969–976. doi: 10.1128/jb.126.2.969-976.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hebeler B. H., Young F. E. Autolysis of Neisseria gonorrhoeae. J Bacteriol. 1975 May;122(2):385–392. doi: 10.1128/jb.122.2.385-392.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Morse S. A., Bartenstein L. Factors affecting autolysis of Neisseria gonorrhoeae. Proc Soc Exp Biol Med. 1974 Apr;145(4):1418–1421. doi: 10.3181/00379727-145-38025. [DOI] [PubMed] [Google Scholar]
  6. Morse S. A., Fitzgerald T. J. Effect of progesterone on Neisseria gonorrhoeae. Infect Immun. 1974 Dec;10(6):1370–1377. doi: 10.1128/iai.10.6.1370-1377.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Morse S. A., Stein S., Hines J. Glucose metabolism in Neisseria gonorrhoeae. J Bacteriol. 1974 Nov;120(2):702–714. doi: 10.1128/jb.120.2.702-714.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Senff L. M., Wegener W. S., Brooks G. F., Finnerty W. R., Makula R. A. Phospholipid composition and phospholipase A activity of Neisseria gonorrhoeae. J Bacteriol. 1976 Aug;127(2):874–880. doi: 10.1128/jb.127.2.874-880.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sud I. J., Feingold D. S. Phospholipids and fatty acids of Neisseria gonorrhoeae. J Bacteriol. 1975 Nov;124(2):713–717. doi: 10.1128/jb.124.2.713-717.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Walstad D. L., Reitz R. C., Sparling P. F. Growth inhibition among strains of Neisseria gonorrhoeae due to production of inhibitory free fatty acids and lysophosphatidylethanolamine: absence of bacteriocins. Infect Immun. 1974 Sep;10(3):481–488. doi: 10.1128/iai.10.3.481-488.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Wegener W. S., Hebeler B. H., Morse S. A. Cell envelope of Neisseria gonorrhoeae: relationship between autolysis in buffer and the hydrolysis of peptidoglycan. Infect Immun. 1977 Oct;18(1):210–219. doi: 10.1128/iai.18.1.210-219.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. White D. C. Lipid composition of the electron transport membrane of Haemophilus parainfluenzae. J Bacteriol. 1968 Oct;96(4):1159–1170. doi: 10.1128/jb.96.4.1159-1170.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES