Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1978 May;20(2):421–429. doi: 10.1128/iai.20.2.421-429.1978

Immunochemical studies on a Mycoplasma pneumoniae polysaccharide fraction: cross-reactions with type 23 and 32 antipneumococcal rabbit sera.

P Z Allen, B Prescott
PMCID: PMC421873  PMID: 27459

Abstract

Lipid-free polysaccharide fraction 2 extracted from Mycoplasma pneumoniae strain FH by Prescott et al. (J. Bacteriol. 91:2117-2115, 1966) was examined for its ability to cross-precipitate antibody from type-specific rabbit antipneumococcal sera types 1 to 34 inclusive. Cross-precipitation in type-specific pneumococcal anti-type 23 and anti-type 32 sera was examined in detail and could be attributed to a rhamnose-galactose-rich component of crude M. pneumoniae polysaccharide fraction 2 recovered from immunoprecipitates formed with anti-type 23 serum. Immunochemically isolated mycoplasma polysaccharide was found to contain glucose, galactose, rhamnose, and mannose in 1:14:5:4 molar proportions. Comparison of the ability of 6-O-alpha-L-rhamnosyl-D-glucose and free L-rhamnose to inhibit precepitation by homologous pneumococcal and heterologous mycoplasma polysaccharide antigens indicates a combining site specificity for anti-type 23 and anti-type 32 antibodies directed largely against the alpha-linked L-rhamnosyl determinants and the occurrence of alpha-L-rhamnosyl units in type 32 and M. pneumoniae polysaccharides. Hapten inhibition of the cross-precipitation of pneumococcal type 23 capsular polysaccharide in anti-type 32 serum helps to establish that cross-reactivity can be attributed to interaction of recurrent, alpha-L-rhamnosyl units of type 23 with anit-alpha-L-rhamnoside combining sites of anti-type 32 antibodies.

Full text

PDF
421

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Caldes G., Prescott B. A comparison of the chemical composition of Mycobacterium tuberculosis muris with Mycobacterium tuberculosis bovis (BCG). Biochem Biophys Res Commun. 1971 Aug 20;44(4):852–858. doi: 10.1016/0006-291x(71)90789-3. [DOI] [PubMed] [Google Scholar]
  2. Felton L. D., Kauffmann G., Stahl H. J. The Precipitation of Bacterial Polysaccharides with Calcium Phosphate : Pneumococcus. J Bacteriol. 1935 Feb;29(2):149–161. doi: 10.1128/jb.29.2.149-161.1935. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Heidelberger M., Davie J. M., Krause R. M. Cross-reactions of the group-specific polysaccharides of streptococcal groups B and G in anti-pneumococcal sera with especial reference to type 23 and its determinants. J Immunol. 1967 Oct;99(4):794–796. [PubMed] [Google Scholar]
  4. Heidelberger M., Jann K., Jann B., Orskov F., Orskov I., Westphal O. Relations between structures of three K polysaccharides of Escherichia coli and cross-reactivity in antipneumococcal sera. J Bacteriol. 1968 Jun;95(6):2415–2417. doi: 10.1128/jb.95.6.2415-2417.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. KABAT E. A., SCHIFFMAN G. Immunochemical studies on blood groups. 28. Further studies on the oligosaccharide determinats of blood group B and BP1 specificity. J Immunol. 1962 Jun;88:782–787. [PubMed] [Google Scholar]
  6. Michel M. F., Krause R. M. Immunochemical studies on the group and type antigens of group F streptococci and the identification of a grouplike carbohydrate in a type II strain with an undesignated group antigen. J Exp Med. 1967 Jun 1;125(6):1075–1089. doi: 10.1084/jem.125.6.1075. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Prescott B., Sobeslavsky O., Caldes G., Chanock R. M. Isolation and characterization of fractions of Mycoplasma pneumoniae. I. Chemical and chromatographic separation. J Bacteriol. 1966 Jun;91(6):2117–2125. doi: 10.1128/jb.91.6.2117-2125.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. SHABAROVA Z. A., BUCHANAN J. G., BADDILEY J. The composition of pneumococcus type-specific substances containing phosphorus. Biochim Biophys Acta. 1962 Feb 12;57:146–148. doi: 10.1016/0006-3002(62)91091-0. [DOI] [PubMed] [Google Scholar]
  9. Schiefer H. G., Gerhardt U., Brunner H., Krüpe M. Studies with lectins on the surface carbohydrate structures of mycoplasma membranes. J Bacteriol. 1974 Oct;120(1):81–88. doi: 10.1128/jb.120.1.81-88.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sobeslavsky O., Prescott B., James W. D., Chanock R. M. Isolation and characterization of fractions of Mycoplasma pneumoniae. II. Antigenicity and immunogenicity. J Bacteriol. 1966 Jun;91(6):2126–2138. doi: 10.1128/jb.91.6.2126-2138.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sobeslavský O., Prescott B., James W. D., Chanock R. M. Serological and immunogenic activities of different fractions of Mycoplasma pneumoniae. Ann N Y Acad Sci. 1967 Jul 28;143(1):682–690. doi: 10.1111/j.1749-6632.1967.tb27714.x. [DOI] [PubMed] [Google Scholar]
  12. TREVELYAN W. E., PROCTER D. P., HARRISON J. S. Detection of sugars on paper chromatograms. Nature. 1950 Sep 9;166(4219):444–445. doi: 10.1038/166444b0. [DOI] [PubMed] [Google Scholar]
  13. WILLERS J. M., OTTENS H., MICHEL M. F. IMMUNOCHEMICAL RELATIONSHIP BETWEEN STREPTOCOCCUS MG, F 3 AND STREPTOCOCCUS SALIVARIUS. J Gen Microbiol. 1964 Dec;37:425–431. doi: 10.1099/00221287-37-3-425. [DOI] [PubMed] [Google Scholar]
  14. Welch H., Borman E. K., Mickle F. L. Preparation and Analysis of Diagnostic Antipneumococcus Serum. Am J Public Health Nations Health. 1939 Jan;29(1):35–42. doi: 10.2105/ajph.29.1.35. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES