Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1978 Jun;20(3):721–727. doi: 10.1128/iai.20.3.721-727.1978

Water-soluble adjuvant obtained from Bacterionema matruchotii.

T Nitta, S Okumura, M J Tanabe, M Nakano
PMCID: PMC421918  PMID: 352955

Abstract

The adjuvant effect of a butanol-extracted water-soluble adjuvant (bu-WSA) obtained from Bacterionemia matruchotii, a gram-positive oral bacteria, was studied on the antibody response at the plaque-forming cell (PFC) level in murine spleens. Intraperitoneal injection of Bu-WSA caused significant increase in direct PFC numbers in spleens 1 to 3 days after the antigenic stimulation with sheep erythrocytes (SRBC). Injection of 100 to 800 microgram of Bu-WSA was effective, and 400 microgram of Bu-WSA seemed to be the optimum for induction of the adjuvant effect. The adjuvant effect was strongest when Bu-WSA was injected at the same time as the SRBC, but some effect was still observed when Bu-WSA was injected 7 days before or 1 day after the immunization. The adjuvant effect of Bu-WSA was greatest at high dose of antigen. The mice injected with Bu-WSA at the time of priming SRBC and then immunized with trinitrophenylated SRBC showed greater anti-trinitrophenyl PFC response than controls without the injection of Bu-WSA. These findings suggest that a part of the adjuvant effect of Bu-WSA depends on thymic cell function and another part does not.

Full text

PDF
721

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adam A., Ciorbaru R., Petit J. F., Lederer E. Isolation and properties of a macromolecular, water-soluble, immuno-adjuvant fraction from the cell wall of Mycobacterium smegmatis. Proc Natl Acad Sci U S A. 1972 Apr;69(4):851–854. doi: 10.1073/pnas.69.4.851. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Andersson J., Melchers F., Galanos C., Lüderitz O. The mitogenic effect of lipopolysaccharide on bone marrow-derived mouse lymphocytes. Lipid A as the mitogenic part of the molecule. J Exp Med. 1973 Apr 1;137(4):943–953. doi: 10.1084/jem.137.4.943. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Andersson J., Sjöberg O., Möller G. Induction of immunoglobulin and antibody synthesis in vitro by lipopolysaccharides. Eur J Immunol. 1972 Aug;2(4):349–353. doi: 10.1002/eji.1830020410. [DOI] [PubMed] [Google Scholar]
  4. Behling U. H., Nowotny A. Immune adjuvancy of lipopolysaccharide and a nontoxic hydrolytic product demonstrating oscillating effects with time. J Immunol. 1977 May;118(5):1905–1907. [PubMed] [Google Scholar]
  5. Hiu I. J. The adjuvant active fraction of delipidated mycobacteria. Nature. 1977 Jun 23;267(5613):708–709. doi: 10.1038/267708a0. [DOI] [PubMed] [Google Scholar]
  6. Horton J. E., Oppenheim J. J., Mergenhagen S. E. A role for cell-mediated immunity in the pathogenesis of periodontal disease. J Periodontol. 1974 May;45(5):351–360. doi: 10.1902/jop.1974.45.5.351. [DOI] [PubMed] [Google Scholar]
  7. Howard J. G., Christie G. H., Scott M. T. Biological effects of Corynebacterium parvum. IV. Adjuvant and inhibitory activities on B lymphocytes. Cell Immunol. 1973 May;7(2):290–301. doi: 10.1016/0008-8749(73)90251-7. [DOI] [PubMed] [Google Scholar]
  8. KIND L. S. Relationship of the anaphylaxis sensitizing and adjuvant properties of Hemophilus pertussis vaccine. J Immunol. 1957 Sep;79(3):238–242. [PubMed] [Google Scholar]
  9. Kotani S., Narita T., Stewart-Tull D. E., Shimono T., Watanabe Y. Immunoadjuvant activities of cell walls and their water-soluble fractions prepared from various gram-positive bacteria. Biken J. 1975 Jun;18(2):77–92. [PubMed] [Google Scholar]
  10. Kumazawa Y., Shibusawa A., Suzuki T., Mizunoe K. Separation of an adjuvant-active glycolipid lacking peptide moiety from wax D preparation of Mycobacterium tuberculosis strain aoyama B. Immunochemistry. 1976 Feb;13(2):173–177. doi: 10.1016/0019-2791(76)90286-x. [DOI] [PubMed] [Google Scholar]
  11. MADDY A. H. THE SOLUBILISATION OF THE PROTEIN OF THE OX-ERYTHROCYTE GHOST. Biochim Biophys Acta. 1964 Sep 25;88:448–449. doi: 10.1016/0926-6577(64)90204-9. [DOI] [PubMed] [Google Scholar]
  12. MUNOZ J. EFFECT OF BACTERIA AND BACTERIAL PRODUCTS ON ANTIBODY RESPONSE. Adv Immunol. 1964;27:396–440. [PubMed] [Google Scholar]
  13. Marx J. L. Suppressor T cells: role in immune regulation. Science. 1975 Apr 18;188(4185):245–247. doi: 10.1126/science.188.4185.245. [DOI] [PubMed] [Google Scholar]
  14. Modolell M., Luckenbach G. A., Parant M., Munder P. G. The adjuvant activity of a mycobacterial water soluble adjuvant (WSA) in vitro. I. The requirement of macrophages. J Immunol. 1974 Jul;113(1):395–403. [PubMed] [Google Scholar]
  15. Morrison D. C., Leive L. Fractions of lipopolysaccharide from Escherichia coli O111:B4 prepared by two extraction procedures. J Biol Chem. 1975 Apr 25;250(8):2911–2919. [PubMed] [Google Scholar]
  16. Murgo A. J., Athanassiades T. J. Adjuvant effect of Bordetella pertussis vaccine to sheep erythrocytes: enhancement of antibody formation by using subcutaneous administration of adjuvant and antigen. Infect Immun. 1975 Nov;12(5):969–977. doi: 10.1128/iai.12.5.969-977.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Nagoya T., Kobayashi F., Nomoto K. Immunological properties of Propionibacterium acnes. I. Potentiation and Suppression on antibody response to sheep and hamster erythrocytes in mice. Microbiol Immunol. 1977;21(1):33–44. doi: 10.1111/j.1348-0421.1977.tb02805.x. [DOI] [PubMed] [Google Scholar]
  18. Nakano M., Shimamura T., Saito K. Cellular mechanisms of adjuvant action of bacterial lipopolysaccharide in anti-sheep red blood cell antibody response. Jpn J Microbiol. 1971 Mar;15(2):149–158. doi: 10.1111/j.1348-0421.1971.tb00564.x. [DOI] [PubMed] [Google Scholar]
  19. Nakashima I., Kobayashi T., Kato N. Alterations in the antibody response to bovine serum albumin by capsular polysaccharide of Klebsiella pneumoniae. J Immunol. 1971 Oct;107(4):1112–1121. [PubMed] [Google Scholar]
  20. Neter E. Endotoxins and the immune response. Curr Top Microbiol Immunol. 1969;47:82–124. doi: 10.1007/978-3-642-46160-6_5. [DOI] [PubMed] [Google Scholar]
  21. Nitta T., Okumura S., Nakano M. Effect of Bacterionema matruchotii on immune responses. I. Enhancement of phagocytic and bactericidal functions. Nihon Saikingaku Zasshi. 1977 Sep;32(5):691–696. [PubMed] [Google Scholar]
  22. Okada H., Inui M., Kawagoe K., Nakata H., Aono M. Adjuvant effects of oral microorganisms on the immunological responses. Arch Oral Biol. 1974 Sep;19(9):761–766. doi: 10.1016/0003-9969(74)90163-0. [DOI] [PubMed] [Google Scholar]
  23. Rittenberg M. B., Pratt K. L. Antitrinitrophenyl (TNP) plaque assay. Primary response of Balb/c mice to soluble and particulate immunogen. Proc Soc Exp Biol Med. 1969 Nov;132(2):575–581. doi: 10.3181/00379727-132-34264. [DOI] [PubMed] [Google Scholar]
  24. SCHERP H. W. Discussion of bacterial factors in periodontal disease. J Dent Res. 1962 Jan-Feb;41:327–330. doi: 10.1177/00220345620410014301. [DOI] [PubMed] [Google Scholar]
  25. Saito T., Nakano M. Effect of bacterial lipopolysaccharides on anti-trinitrophenyl antibody-producing cells. Nonspecific modification of the affinity at the cellular level. Jpn J Microbiol. 1975 Dec;19(6):419–425. doi: 10.1111/j.1348-0421.1975.tb00957.x. [DOI] [PubMed] [Google Scholar]
  26. Schwab J. H. Suppression of the immune response by microorganisms. Bacteriol Rev. 1975 Jun;39(2):121–143. doi: 10.1128/br.39.2.121-143.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Sterzl J., Ríha I. Detection of cells producing 7S antibodies by the plaque technique. Nature. 1965 Nov 27;208(5013):858–859. doi: 10.1038/208858a0. [DOI] [PubMed] [Google Scholar]
  28. Warr G. W., James K. Effect of Corynebacterium parvum on the class and subclass of antibody produced in the response of different strains of mice to sheep erythrocytes. Immunology. 1975 Mar;28(3):431–442. [PMC free article] [PubMed] [Google Scholar]
  29. Warr G. W., Sljivić V. S. Enhancement and depression of the antibody response in mice caused by Corynebacterium parvum. Clin Exp Immunol. 1974 Jul;17(3):519–532. [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES