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. 1978 Jun;20(3):732–738. doi: 10.1128/iai.20.3.732-738.1978

Quantitative measurement of phagocytosis of Neisseria gonorrhoeae by mouse peritoneal macrophages.

R B Jones, T M Buchanan
PMCID: PMC421920  PMID: 97235

Abstract

The simultaneous labeling of gonococci with [6-3H]uracil and of mouse peritoneal macrophages with L-[U-14C]leucine permits a quantitative assessment of the association of gonococci with macrophages under various experimental conditions. Colony-type T1 (piliated) gonococci associated more than T4 (nonpiliated) organisms at 4 degrees C, but at 37 degrees C the association of T4 gonococci with macrophages exceeded that for the T1 organisms. The association of T1 gonococci with macrophages could be enhanced as much as 70-fold by homologous rabbit antisera prepared against whole, formaldehyde-treated organisms. This immune enhancement represented primarily increased phagocytosis rather than surface attachment, as shown by its inhibition at 4 degrees C or with 2-deoxyglucose. The data further suggested that this enhanced phagocytosis was mediated via the Fc receptor for immunoglobulin G.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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