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. 1978 Jun;20(3):842–846. doi: 10.1128/iai.20.3.842-846.1978

Measles virus nucleocapsids: large-scale purification and use in radioimmunoassays.

P M Moore, E C Hayes, S E Miller, L L Wright, C E Machamer, H J Zweerink
PMCID: PMC421934  PMID: 352962

Abstract

Nucleocapsids in quantities approaching 1 mg were purified from 109 measles virus-infected cells. They contained one polypeptide species with a molecular weight of 59,000. Antiserum was raised in rabbits against purified nucleocapsids and used in a competitive radioimmunoassay. Because of their instability, purified nucleocapsids were not suitable for use in such an assay. Instead partially purified nucleocapsids from HEp-2 cells persistently infected with measles virus and labeled in vitro with [35S]methionine were used as the source of radioactive antigen. The radioimmunoassay thus developed measured less than 5 ng of nucleocapsids in infected cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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