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. 1978 Jun;20(3):842–846. doi: 10.1128/iai.20.3.842-846.1978

Measles virus nucleocapsids: large-scale purification and use in radioimmunoassays.

P M Moore, E C Hayes, S E Miller, L L Wright, C E Machamer, H J Zweerink
PMCID: PMC421934  PMID: 352962

Abstract

Nucleocapsids in quantities approaching 1 mg were purified from 109 measles virus-infected cells. They contained one polypeptide species with a molecular weight of 59,000. Antiserum was raised in rabbits against purified nucleocapsids and used in a competitive radioimmunoassay. Because of their instability, purified nucleocapsids were not suitable for use in such an assay. Instead partially purified nucleocapsids from HEp-2 cells persistently infected with measles virus and labeled in vitro with [35S]methionine were used as the source of radioactive antigen. The radioimmunoassay thus developed measured less than 5 ng of nucleocapsids in infected cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADAMS J. M., IMAGAWA D. T. Measles antibodies in multiple sclerosis. Proc Soc Exp Biol Med. 1962 Dec;111:562–566. doi: 10.3181/00379727-111-27855. [DOI] [PubMed] [Google Scholar]
  2. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  3. Brown P., Cathala F., Gajdusek D. C., Gibbs C. J., Jr Measles antibodies in the cerebrospinal fluid of patients with multiple sclerosis. Proc Soc Exp Biol Med. 1971 Jul;137(3):956–961. doi: 10.3181/00379727-137-35704. [DOI] [PubMed] [Google Scholar]
  4. Chang C. T., Zweerink H. J. Fate of parental reovirus in infected cell. Virology. 1971 Dec;46(3):544–555. doi: 10.1016/0042-6822(71)90058-4. [DOI] [PubMed] [Google Scholar]
  5. Hebert G. A., Pelham P. L., Pittman B. Determination of the optimal ammonium sulfate concentration for the fractionation of rabbit, sheep, horse, and goat antisera. Appl Microbiol. 1973 Jan;25(1):26–36. doi: 10.1128/am.25.1.26-36.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kessler S. W. Cell membrane antigen isolation with the staphylococcal protein A-antibody adsorbent. J Immunol. 1976 Nov;117(5 Pt 1):1482–1490. [PubMed] [Google Scholar]
  7. Kessler S. W. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol. 1975 Dec;115(6):1617–1624. [PubMed] [Google Scholar]
  8. Morgan E. M., Rapp F. Measles virus and its associated diseases. Bacteriol Rev. 1977 Sep;41(3):636–666. doi: 10.1128/br.41.3.636-666.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mountcastle W. E., Choppin P. W. A comparison of the polypeptides of four measles virus strains. Virology. 1977 May 15;78(2):463–474. doi: 10.1016/0042-6822(77)90123-4. [DOI] [PubMed] [Google Scholar]
  10. Mountcastle W. E., Compans R. W., Caliguiri L. A., Choppin P. W. Nucleocapsid protein subunits of simian virus 5, Newcastle disease virus, and Sendai virus. J Virol. 1970 Nov;6(5):677–684. doi: 10.1128/jvi.6.5.677-684.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mountcastle W. E., Compans R. W., Lackland H., Choppin P. W. Proteolytic cleavage of subunits of the nucleocapsid of the paramyxovirus simian virus 5. J Virol. 1974 Nov;14(5):1253–1261. doi: 10.1128/jvi.14.5.1253-1261.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nakai M., Imagawa D. T. Electron microscopy of measles virus replication. J Virol. 1969 Feb;3(2):187–197. doi: 10.1128/jvi.3.2.187-197.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Norrby E., Link H., Olsson J. E. Measles virus antibodies in multiple sclerosis. Comparison of antibody titers in cerebrospinal fluid and serum. Arch Neurol. 1974 Apr;30(4):285–292. doi: 10.1001/archneur.1974.00490340013002. [DOI] [PubMed] [Google Scholar]
  14. PATTERSON M. S., GREENE R. C. MEASUREMENT OF LOW ENERGY BETA-EMITTERS IN AQUEOUS SOLUTION BY LIQUID SCINTILLATION COUNTING OF EMULSIONS. Anal Chem. 1965 Jun;37:854–857. doi: 10.1021/ac60226a017. [DOI] [PubMed] [Google Scholar]
  15. Panelius M., Salmi A., Halonen P. E., Kivalo E., Rinne U. K., Penttinen K. Virus antibodies in serum specimens from patients with multiple sclerosis, from siblings, and matched controls. A final report. Acta Neurol Scand. 1973;49(1):85–107. doi: 10.1111/j.1600-0404.1973.tb01281.x. [DOI] [PubMed] [Google Scholar]
  16. Paty D. W., Furesz J., Boucher D. W., Rand C. G., Stiller C. R. Measles antibodies as related to HL-A types in multiple sclerosis. Neurology. 1976 Jul;26(7):651–655. doi: 10.1212/wnl.26.7.651. [DOI] [PubMed] [Google Scholar]
  17. Prineas J. Paramyxovirus-like particles associated with acute demyelination in chronic relapsing multiple sclerosis. Science. 1972 Nov 17;178(4062):760–763. doi: 10.1126/science.178.4062.760. [DOI] [PubMed] [Google Scholar]
  18. Raine C. S., Powers J. M., Suzuki K. Acute multiple sclerosis. Confirmation of "paramyxovirus-like" intranuclear inclusions. Arch Neurol. 1974 Jan;30(1):39–46. doi: 10.1001/archneur.1974.00490310041007. [DOI] [PubMed] [Google Scholar]
  19. Smith R. E., Zweerink H. J., Joklik W. K. Polypeptide components of virions, top component and cores of reovirus type 3. Virology. 1969 Dec;39(4):791–810. doi: 10.1016/0042-6822(69)90017-8. [DOI] [PubMed] [Google Scholar]
  20. Waters D. J., Bussell R. H. Isolation and comparative study of the nucleocapsids of measles and canine distemper viruses from infected cells. Virology. 1974 Sep;61(1):74–79. [PubMed] [Google Scholar]

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