Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1978 Jul;21(1):55–58. doi: 10.1128/iai.21.1.55-58.1978

Immunoglobulin G and immunoglobulin M enzyme-linked immunosorbent assays and defined toxoplasmosis serological patterns.

M E Camargo, A W Ferreira, J R Mineo, C K Takiguti, O S Nakahara
PMCID: PMC421956  PMID: 361569

Abstract

Enzyme-linked immunosorbent assay (ELISA) for toxoplasmosis was evaluated in serum samples presenting defined toxoplasmosis serological patterns, as determined by results in immunoglobulin (Ig)G and IgM immunofluorescence (IgG-IF, IgM-IF), hemagglutination, and complement fixation tests. ELISA was carried out with alkaline phosphatase-labeled anti-IgG and anti-IgM antibodies. Serum titer was expressed as the serum dilution end point determined by mere observation of color development in the test. A straight agreement was found between IgG-ELISA and IgG-IF titers, both in group A sera of ancient infections (patterns II and III) and group B sera of recent infections (pattern I). A similar agreement was found between IgG-ELISA and hemagglutination titers in group A sera, for which coincident IgG-IF and hemagglutination titers are also frequent. However, in group B sera, in spite of the same toxoplasma extract being used to sensitize both plastic surfaces and erythrocytes, IgG-ELISA titers were much higher than hemagglutination titers, in a way similar to that observed for IgG-IF titers. IgM-ELISA was positive in every group B serum, with higher titers than corresponding IgM-IF titers. Occasional low-titered positive IgM-ELISA results were seen for group A sera, sometimes due to IgM-antiglobulin antibodies. An easy test to perform, ELISA seems to be an adequate substitute for toxoplasmosis IF tests for routine purposes.

Full text

PDF
55

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avrameas S. Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies. Immunochemistry. 1969 Jan;6(1):43–52. doi: 10.1016/0019-2791(69)90177-3. [DOI] [PubMed] [Google Scholar]
  2. Bout D., Dugimont J. C., Farag H., Capron A. Le diagnostic immunoenzymologique des affections parasitaires. II - Immunoenzymologie quantitative sur lame. Lille Med. 1975 Jun-Jul;20(6):561–566. [PubMed] [Google Scholar]
  3. Camargo M. E., Leser P. G. Diagnostic information from serological tests in human toxoplasmosis. II Evolutive study of antibodies and serological patterns in acquired toxoplasmosis, as detected by hemagglutination, complement fixation, IgG and IgM-immunofluorescence tests. Rev Inst Med Trop Sao Paulo. 1976 Jul-Aug;18(4):227–238. [PubMed] [Google Scholar]
  4. Camargo M. E., Leser P. G., Leser W. S. Diagnostic information from serological tests in human toxoplasmosis. I. A comparative study of hemagglutination, complement fixation, IgG and IgM-immunofluorescence tests in 3,752 serum samples. Rev Inst Med Trop Sao Paulo. 1976 Jul-Aug;18(4):215–226. [PubMed] [Google Scholar]
  5. Camargo M. E., Leser P. G., Rocca A. Diagnostic information from serological tests in human toxoplasmosis. III.--Hemagglutination titers paradoxical decrease in acute infections. Rev Inst Med Trop Sao Paulo. 1978 Jan-Feb;20(1):41–44. [PubMed] [Google Scholar]
  6. Carlsson H. E., Lindberg A. A., Hammarström S. Titration of antibodies to salmonella O antigens by enzyme-linked immunosorbent assay. Infect Immun. 1972 Nov;6(5):703–708. doi: 10.1128/iai.6.5.703-708.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
  8. Karim K. A., Ludlam G. B. The relationship and significance of antibody titres as determined by various serological methods in glandular and ocular toxoplasmosis. J Clin Pathol. 1975 Jan;28(1):42–49. doi: 10.1136/jcp.28.1.42. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Voller A., Bidwell D. E., Bartlett A., Fleck D. G., Perkins M., Oladehin B. A microplate enzyme-immunoassay for toxoplasma antibody. J Clin Pathol. 1976 Feb;29(2):150–153. doi: 10.1136/jcp.29.2.150. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Walls K. W., Bullock S. L., English D. K. Use of the enzyme-linked immunosorbent assay (ELISA) and its microadaptation for the serodiagnosis of toxoplasmosis. J Clin Microbiol. 1977 Mar;5(3):273–277. doi: 10.1128/jcm.5.3.273-277.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES