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. 1978 Aug;21(2):381–386. doi: 10.1128/iai.21.2.381-386.1978

Polymorphonuclear neutrophil chemotaxis under aerobic and anaerobic conditions.

D A Casciato, L S Goldberg, R Bluestone
PMCID: PMC422006  PMID: 357284

Abstract

The motility of human polymorphonuclear neutrophils was studied in vitro under aerobic and anaerobic conditions. Chemotactic factors were generated from plasma with immune complexes or with whole bacteria (Staphylococcus aureus, Escherichia coli, and Bacteroides fragilis). Chemotaxis induced by chemotactic factors generated from immune complexes was identical under both conditions. However, chemotaxis utilizing chemotactic factors generated from bacteria was markedly depressed under anaerobic conditions. Mean random tubemoltility was not significantly different under aerobic and anaerobic conditions. These data indicate that different metabolic pathways may be involved in polymorphonuclear neutrophil movement. Some of these pathways require oxygen (chemotaxis in response to factors generated by bacteria in plasma), whereas others do not (random tube migration and chemotaxis in response to factors generated by immune complexes in plasma). These observations may be important in the induction of inflammatory responses within hypoxic tissues.

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Selected References

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  1. Arank A., Syed S. A., Kenney E. B., Freter R. Isolation of anaerobic bacteria from human gingiva and mouse cecum by means of a simplified glove box procedure. Appl Microbiol. 1969 Apr;17(4):568–576. doi: 10.1128/am.17.4.568-576.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bryant R. E., DesPrez R. M., VanWay M. H., Rogers D. E. Studies on human leukocyte motility. I. Effects of alterations in pH, electrolyte concentration, and phagocytosis on leukocyte migration, adhesiveness, and aggregation. J Exp Med. 1966 Sep 1;124(3):483–499. doi: 10.1084/jem.124.3.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CAGAN R. H., KARNOVSKY M. L. ENZYMATIC BASIS OF THE RESPIRATORY STIMULATION DURING PHAGOCYTOSIS. Nature. 1964 Oct 17;204:255–257. doi: 10.1038/204255a0. [DOI] [PubMed] [Google Scholar]
  4. Carruthers B. M. Leukocyte motility. I. Method of study, normal variation, effect of physical alterations in environment, and effect of iodoacetate. Can J Physiol Pharmacol. 1966 May;44(3):475–485. doi: 10.1139/y66-056. [DOI] [PubMed] [Google Scholar]
  5. Carruthers B. M. Leukocyte motility. II. Effect of absence of glucose in medium; effect of presence of deoxyglucose, dinitrophenol, puromycin, actinomycin D, and trypsin on the response to chemotactic substance; effect of segregation of cells from chemotactic substance. Can J Physiol Pharmacol. 1967 Mar;45(2):269–280. doi: 10.1139/y67-029. [DOI] [PubMed] [Google Scholar]
  6. KETCHEL M. M., FAVOUR C. B. The acceleration and inhibition of migration of human leucocytes in vitro by plasma protein fractions. J Exp Med. 1955 Jun 1;101(6):647–663. doi: 10.1084/jem.101.6.647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Keller H. U., Wilkinson P. C., Abercrombie M., Becker E. L., Hirsch J. G., Miller M. E., Ramsey W. S., Zigmond S. H. A proposal for the definition of terms related to locomotion of leukocytes and other cells. J Immunol. 1977 May;118(5):1912–1914. [PubMed] [Google Scholar]
  8. Klebanoff S. J. Antimicrobial mechanisms in neutrophilic polymorphonuclear leukocytes. Semin Hematol. 1975 Apr;12(2):117–142. [PubMed] [Google Scholar]
  9. Kraal J. H., Kenney E. B. Polymorphonuclear leukocyte chemotaxis in the absence of oxygen. J Periodontal Res. 1975 Nov;10(5):288–293. doi: 10.1111/j.1600-0765.1975.tb00036.x. [DOI] [PubMed] [Google Scholar]
  10. McRipley R. J., Sbarra A. J. Role of the phagocyte in host-parasite interactions. XI. Relationship between stimulated oxidative metabolism and hydrogen peroxide formation, and intracellular killing. J Bacteriol. 1967 Nov;94(5):1417–1424. doi: 10.1128/jb.94.5.1417-1424.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Miller M. E., Norman M. E., Koblenzer P. J., Schonauer T. A new familial defect of neutrophil movement. J Lab Clin Med. 1973 Jul;82(1):1–8. [PubMed] [Google Scholar]
  12. Snyderman R., Phillips J., Mergenhagen S. E. Polymorphonuclear leukocyte chemotactic activity in rabbit serum and Guinea pig serum treated with immune complexes: evidence for c5a as the major chemotactic factor. Infect Immun. 1970 Jun;1(6):521–525. doi: 10.1128/iai.1.6.521-525.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Snyderman R., Shin H. S., Phillips J. K., Gewurz H., Mergenhagen S. E. A neutrophil chemotatic factor derived from C'5 upon interaction of guinea pig serum with endotoxin. J Immunol. 1969 Sep;103(3):413–422. [PubMed] [Google Scholar]
  14. Walker W. S., Barlet R. L., Kurtz H. M. Isolation and partial characterization of a staphylococcal leukocyte cytotaxin. J Bacteriol. 1969 Mar;97(3):1005–1008. doi: 10.1128/jb.97.3.1005-1008.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ward P. A. A plasmin-split fragment of C'3 as a new chemotactic factor. J Exp Med. 1967 Aug 1;126(2):189–206. doi: 10.1084/jem.126.2.189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ward P. A., Newman L. J. A neutrophil chemotactic factor from human C'5. J Immunol. 1969 Jan;102(1):93–99. [PubMed] [Google Scholar]
  17. Ward P. A. The chemosuppression of chemotaxis. J Exp Med. 1966 Aug 1;124(2):209–226. doi: 10.1084/jem.124.2.209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Weksler B. B., Hill M. J. Inhibition of leukocyte migration by a staphylococcal factor. J Bacteriol. 1969 Jun;98(3):1030–1035. doi: 10.1128/jb.98.3.1030-1035.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wilkinson P. C. Inhibition of leukocyte locomotion and chemotaxis by lipid-specific bacterial toxins. Nature. 1975 Jun 5;255(5508):485–487. doi: 10.1038/255485a0. [DOI] [PubMed] [Google Scholar]

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