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. 1978 Aug;21(2):387–391. doi: 10.1128/iai.21.2.387-391.1978

Isolation of specific and common antibodies to staphylococcal enterotoxins A and E by affinity chromatography.

A C Lee, R N Robbins, M S Bergdoll
PMCID: PMC422007  PMID: 689729

Abstract

Specific and common antibodies to staphylococcal enterotoxins A and E (SEA and SEE) were isolated from anti-SEA and anti-SEE antisera by affinity chromatography. Anti-SEA and anti-SEE antisera were passed through a column with the cross-reacting enterotoxin coupled to a CNBr-activated Sepharose 4B matrix. Specifically bound common antibodies were eluted with NaSCN. The isolated specific antibodies reacted only with the homologous enterotoxin, whereas the common antibodies gave a reaction of identify with both enterotoxins in double gel diffusion plates. The common antibodies had a higher titer against SEE than against SEA. The significance of the isolation of antibodies common to two separate protein molecules is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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