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. 1978 Aug;21(2):514–517. doi: 10.1128/iai.21.2.514-517.1978

Stimulation of cyclic AMP secretion in Vero cells by enterotoxins of Escherichia coli and Vibrio cholerae.

S Stavric, J I Speirs, J Konowalchuk, D Jeffrey
PMCID: PMC422025  PMID: 80381

Abstract

The morphological response of Vero cells to Escherichia coli heat-labile enterotoxin was similar to that of cholera toxin and was accompanied by increases in the intracellular level of cyclic AMP. The effects of both enterotoxins were enhanced by the presence of phosphodiesterase inhibitor and inhibited by heat or specific antisera. Accumulation of cyclic AMP preceded the morphological response.

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Selected References

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  1. BUTCHER R. W., SUTHERLAND E. W. Adenosine 3',5'-phosphate in biological materials. I. Purification and properties of cyclic 3',5'-nucleotide phosphodiesterase and use of this enzyme to characterize adenosine 3',5'-phosphate in human urine. J Biol Chem. 1962 Apr;237:1244–1250. [PubMed] [Google Scholar]
  2. Boyle J. M., Gardner J. D. Sequence of events mediating the effect of cholera toxin on rat thymocytes. J Clin Invest. 1974 Apr;53(4):1149–1158. doi: 10.1172/JCI107653. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Desbuquois B., Aurbach G. D. Use of polyethylene glycol to separate free and antibody-bound peptide hormones in radioimmunoassays. J Clin Endocrinol Metab. 1971 Nov;33(5):732–738. doi: 10.1210/jcem-33-5-732. [DOI] [PubMed] [Google Scholar]
  4. Donta S. T. Comparison of the effects of cholera enterotoxin and ACTH on adrenal cells in tissue culture. Am J Physiol. 1974 Jul;227(1):109–113. doi: 10.1152/ajplegacy.1974.227.1.109. [DOI] [PubMed] [Google Scholar]
  5. Donta S. T., Moon H. W., Whipp S. C. Detection of heat-labile Escherichia coli enterotoxin with the use of adrenal cells in tissue culture. Science. 1974 Jan 25;183(4122):334–336. doi: 10.1126/science.183.4122.334. [DOI] [PubMed] [Google Scholar]
  6. Dorner F., Mayer P. Escherichia coli enterotoxin: stimulation of adenylate cyclase in broken-cell preparations. Infect Immun. 1975 Mar;11(3):429–435. doi: 10.1128/iai.11.3.429-435.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Evans D. G., Evans D. J., Jr, Pierce N. F. Differences in the response of rabbit small intestine to heat-labile and heat-stable enterotoxins of Escherichia coli. Infect Immun. 1973 Jun;7(6):873–880. doi: 10.1128/iai.7.6.873-880.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gilman A. G. A protein binding assay for adenosine 3':5'-cyclic monophosphate. Proc Natl Acad Sci U S A. 1970 Sep;67(1):305–312. doi: 10.1073/pnas.67.1.305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Guerrant R. L., Brunton L. L., Schnaitman T. C., Rebhun L. I., Gilman A. G. Cyclic adenosine monophosphate and alteration of Chinese hamster ovary cell morphology: a rapid, sensitive in vitro assay for the enterotoxins of Vibrio cholerae and Escherichia coli. Infect Immun. 1974 Aug;10(2):320–327. doi: 10.1128/iai.10.2.320-327.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kantor H. S., Tao P., Wisdom C. Action of Escherichia coli enterotoxin: adenylate cyclase behavior of intestinal epithelial cells in culture. Infect Immun. 1974 Jun;9(6):1003–1010. doi: 10.1128/iai.9.6.1003-1010.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Konowalchuk J., Speirs J. I., Stavric S. Vero response to a cytotoxin of Escherichia coli. Infect Immun. 1977 Dec;18(3):775–779. doi: 10.1128/iai.18.3.775-779.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Li A. P., O'Neill J. P., Kawashima K., Hsie A. W. Correlation between changes in intracellular level of cyclic AMP, activation of cyclic AMP-dependent protein kinase, and the morphology of Chinese hamster ovary cells in culture. Arch Biochem Biophys. 1977 Jul;182(1):181–187. doi: 10.1016/0003-9861(77)90297-1. [DOI] [PubMed] [Google Scholar]
  13. Mashiter K., Mashiter G. D., Hauger R. L., Field J. B. Effects of cholera and E. coli enterotoxins on cyclic adenosine 3',5'-monophosphate levels and intermediary metabolism in the thyroid. Endocrinology. 1973 Feb;92(2):541–549. doi: 10.1210/endo-92-2-541. [DOI] [PubMed] [Google Scholar]
  14. SUTHERLAND E. W., RALL T. W. Fractionation and characterization of a cyclic adenine ribonucleotide formed by tissue particles. J Biol Chem. 1958 Jun;232(2):1077–1091. [PubMed] [Google Scholar]
  15. Speirs J. I., Stavric S., Konowalchuk J. Assay of Escherichia coli heat-labile enterotoxin with vero cells. Infect Immun. 1977 May;16(2):617–622. doi: 10.1128/iai.16.2.617-622.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Stavric S., Jeffrey D. A modified bioassay for heat-stable Escherichia coli enterotoxin1. Can J Microbiol. 1977 Mar;23(3):331–336. doi: 10.1139/m77-049. [DOI] [PubMed] [Google Scholar]
  17. Steiner A. L., Parker C. W., Kipnis D. M. Radioimmunoassay for cyclic nucleotides. I. Preparation of antibodies and iodinated cyclic nucleotides. J Biol Chem. 1972 Feb 25;247(4):1106–1113. [PubMed] [Google Scholar]
  18. Zenser T. V., Metzger J. F. Comparison of the action of Escherichia coli enterotoxin on the thymocyte adenylate cyclase-cyclic adenosine monophosphate system to that of cholera toxin and prostaglandin E1. Infect Immun. 1974 Sep;10(3):503–509. doi: 10.1128/iai.10.3.503-509.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

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