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. 1978 Aug;21(2):546–555. doi: 10.1128/iai.21.2.546-555.1978

Extraction of streptococcal type 12 M protein by cyanogen bromide.

K L Vosti, W K Williams
PMCID: PMC422030  PMID: 357290

Abstract

Conditions for the release of streptococcal type 12 M protein from whole cells by cyanogen bromide are described; they demonstrated that methionine is not essential to the structural arrangements which account for some of its immunological and biological properties. The released M protein was separated from other proteins by column chromatography with hydroxylapatite. The type-specific molecules which reacted with precipitating antibodies were found only in the 0.3 M eluate, formed zones with mobilities less than 12% of that of the dye front on electrophoresis in the standard acrylamide disc gel system, formed at least four bands in sodium dodecyl sulfate-acrylamide disc gels with molecular weights ranging from 12,000 to 23,000, and stimulated the formation of opsonic antibodies in rabbits. Cyanogen bromide provides a highly specific method for the release of M proteins which should prove particularly useful in analyses of structural-functional relationships among different M proteins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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