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. 1978 Sep;21(3):862–865. doi: 10.1128/iai.21.3.862-865.1978

Chemotaxigenic activity of extracts from the mycelial and spherule phases of Coccidioides immitis for human polymorphonuclear leukocytes.

J N Galgiani, R A Isenberg, D A Stevens
PMCID: PMC422076  PMID: 711340

Abstract

In order to further characterize human host defenses against Coccidioides immitis, extracts of this fungus were tested in vitro for their ability to attract polymorphonuclear leukocytes derived from peripheral blood of uninfected subjects. Soluble substances prepared from the mycelial (saprophytic) and spherule (tissue) phases exhibited, in the presence of serum, dose-dependent chemotactic activity. The dose-response correlations were different. The spherule-derived preparation showed decreased activity at the high concentrations, a diminution not observed with equivalent concentrations of the mycelial filtrate. Chemotactic activity was not observed with either substance in the absence of serum or if heat-inactivated serum was substituted. Because the response of human polymorphonuclear leukocytes to these fungal substances appears complement mediated, a selective cellular defect in this function which antedates exposure to C. immitis seems unlikely.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAKER O., BRAUDE A. I. A study of stimuli leading to the production of spherules in coccidioidomycosis. J Lab Clin Med. 1956 Feb;47(2):169–181. [PubMed] [Google Scholar]
  2. Cutler J. E. Chemotactic factor produced by Candida albicans. Infect Immun. 1977 Dec;18(3):568–573. doi: 10.1128/iai.18.3.568-573.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Denning T. J., Davies R. R. Candida albicans and the chemotaxis of polymorphonuclear neutrophils. Sabouraudia. 1973 Nov;11(3):210–221. [PubMed] [Google Scholar]
  4. Deresinski S. C., Levine H. B., Stevens D. A. Soluble antigens of mycelia and spherules in the in vitro detection of immunity to Coccidioides immitis. Infect Immun. 1974 Oct;10(4):700–704. doi: 10.1128/iai.10.4.700-704.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Graybill J. R., Silva J., Jr, Alford R. H., Thor D. E. Immunologic and clinical improvement of progressive coccidioidomycosis following administration of transfer factor. Cell Immunol. 1973 Jul;8(1):120–135. doi: 10.1016/0008-8749(73)90099-3. [DOI] [PubMed] [Google Scholar]
  6. Landay M. E., Wheat R. W., Conant N. F., Lowe E. P. Serological comparison of the three morphological phases of Coccidioides immitis by the agar gel diffusion method. J Bacteriol. 1967 Jan;93(1):1–6. doi: 10.1128/jb.93.1.1-6.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lehrer R. I. Antifungal effects of peroxidase systems. J Bacteriol. 1969 Aug;99(2):361–365. doi: 10.1128/jb.99.2.361-365.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Levine H. B., Cobb J. M., Scalarone G. M. Spherule coccidioidin in delayed dermal sensitivity reactions of experimental animals. Sabouraudia. 1969 Feb;7(1):20–32. doi: 10.1080/00362177085190051. [DOI] [PubMed] [Google Scholar]
  9. Ray J. G., Jr, Converse J. L. Agar-gel precipitin-inhibition test for coccidioidomycosis. II. Serological test antigen studies in agar-gel. Appl Microbiol. 1967 Sep;15(5):1054–1061. doi: 10.1128/am.15.5.1054-1061.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ray T. L., Wuepper K. D. Activation of the alternative (properdin) pathway of complement by Candida albicans and related species. J Invest Dermatol. 1976 Dec;67(6):700–703. doi: 10.1111/1523-1747.ep12598581. [DOI] [PubMed] [Google Scholar]
  11. Stevens D. A., Pappagianis D., Marinkovich V. A., Waddell T. F. Immunotherapy in recurrent coccidioidomycosis. Cell Immunol. 1974 Apr;12(1):37–48. doi: 10.1016/0008-8749(74)90054-9. [DOI] [PubMed] [Google Scholar]
  12. TARBET J. E., BRESLAU A. M. Histochemical investigation of the spherule of Coccidioides immitis in relation to host reaction. J Infect Dis. 1953 Mar-Apr;92(2):183–190. doi: 10.1093/infdis/92.2.183. [DOI] [PubMed] [Google Scholar]
  13. Ward P. A., Lepow I. H., Newman L. J. Bacterial factors chemotactic for polymorphonuclear leukocytes. Am J Pathol. 1968 Apr;52(4):725–736. [PMC free article] [PubMed] [Google Scholar]
  14. Zweiman B., Pappagianis D., Maibach H., Hildreth E. A. Coccidioidin delayed hypersensitivity: skin test and in vitro lymphocyte reactivities. J Immunol. 1969 May;102(5):1284–1289. [PubMed] [Google Scholar]

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