Abstract
BCG cell wall skeletons (SK) derived from BCG cell walls (CW) by treatment with proteolytic enzymes and organic solvents were tested for their potency to cause regression of a transplanted guinea pig hepatoma. On a weight basic, SK were as effective as CW in causing tumor regression, and they, as well as purified protein derivative of mycobacteria, provoked delayed cutaneous hypersensitivity reactions in animals immunized with CW or with SK. On a weight basis, CW were more active than SK in eliciting delayed cutaneous hypersensitivity in sensitized guinea pigs whether the animals were immunized with CW or with SK. In unimmunized animals the inflammatory response to intradermally administered CW was greater than that evoked by SK. CW and SK provoked delayed cutaneous hypersensitivity reactions of similar strength in animals immunized with living BCG. This study provided no compelling reasons for using SK instead of CW in clinical trials of cancer treatment by mycobacterial vaccines.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Azuma I., Ribi E. E., Meyer T. J., Zbar B. Biologically active components from mycobacterial cell walls. I. Isolation and composition of cell wall skeleton and component P3. J Natl Cancer Inst. 1974 Jan;52(1):95–101. doi: 10.1093/jnci/52.1.95. [DOI] [PubMed] [Google Scholar]
- Bast R. C., Jr, Zbar B., Borsos T., Rapp H. J. BCG and cancer (first of two parts). N Engl J Med. 1974 Jun 20;290(25):1413–1420. doi: 10.1056/NEJM197406202902506. [DOI] [PubMed] [Google Scholar]
- Bekierkunst A. Immunotherapy of cancer with nonliving mycobacteria and cord factor (trehalose-6,6'-dimycolate) in aqueous medium. J Natl Cancer Inst. 1976 Oct;57(4):963–964. doi: 10.1093/jnci/57.4.963. [DOI] [PubMed] [Google Scholar]
- Meyer T. J., Ribi E. E., Azuma I., Zbar B. Biologically active components from mycobacterial cell walls. II. Suppression and regression of strain-2 guinea pig hepatoma. J Natl Cancer Inst. 1974 Jan;52(1):103–111. doi: 10.1093/jnci/52.1.103. [DOI] [PubMed] [Google Scholar]
- Ribi E. E., Meyer T. J., Azuma I., Zbar B. Mycobacterial cell wall components in tumor suppression and regression. Natl Cancer Inst Monogr. 1973 Dec;39:115–119. [PubMed] [Google Scholar]
- Rosenberg S. A., Seipp C., Sears H. F. Clinical and immunologic studies of disseminated BCG infection. Cancer. 1978 May;41(5):1771–1780. doi: 10.1002/1097-0142(197805)41:5<1771::aid-cncr2820410519>3.0.co;2-c. [DOI] [PubMed] [Google Scholar]
- Yamamura Y., Yoshizaki K., Azuma I., Yagura T., Watanabe T. Immunotherapy of human malignant melanoma with oil-attached BCG cell-wall skeleton. Gan. 1975 Aug;66(4):355–363. [PubMed] [Google Scholar]
- Yarkoni E., Meltzer M. S., Rapp H. J. Tumor regression after intralesional injection of emulsified trehalose-6,6'-dimycolate (cord factor): efficacy increases with oil concentration. Int J Cancer. 1977 Jun 15;19(6):818–821. doi: 10.1002/ijc.2910190612. [DOI] [PubMed] [Google Scholar]
- Zbar B., Ribi E., Meyer T., Azuma I., Rapp H. J. Immunotherapy of cancer: regression of established intradermal tumors after intralesional injection of mycobacterial cell walls attached to oil droplets. J Natl Cancer Inst. 1974 May;52(5):1571–1577. doi: 10.1093/jnci/52.5.1571. [DOI] [PubMed] [Google Scholar]
- Zbar B., Wepsic H. T., Rapp H. J., Whang-Peng J. Transplantable hepatomas induced in strain-2 guinea pigs by diethylnitrosamine: characterization by histology, growth, and chromosomes. J Natl Cancer Inst. 1969 Oct;43(4):821–831. [PubMed] [Google Scholar]