Crosstalk between TGF-β/Smad3 and BMP/BMPR2 signaling pathways via miR-17~92 cluster in carotid artery restenosis

Abstract

In the past decades, carotid angioplasty and stenting (CAS) has been developed into a credible option for the patients with carotid stenosis. However, restenosis remains a severe and unsolved issue after CAS treatment. Restenosis is characterized by neointimal hyperplasia, which is partially caused by vascular smooth muscle cells (VSMC) proliferation. However, the molecular mechanism involved in the restenosis is still unclear. In this study, we demonstrated a functional crosstalk between two TGF-β superfamily signaling pathway members, Smad3 and BMPR2, in VSMC proliferation. Smad3 plays an important role in the TGF-β/Smad3 signaling pathway, and is significantly up-regulated in the carotid artery with restenosis to promote VSMC proliferation. In contrast, BMP receptor II (BMPR2), an inhibitor of VSMC proliferation is down-regulated in carotid restenosis. We further found that BMPR2 down-regulation is mediated by miR-17~92 cluster, which is transcriptionally regulated by Smad3. Thus, Smad3 up-regulation and Smad3/miR-17~92 cluster -dependent BMPR2 down-regulation are likely to promote VSMC proliferation and restenosis. Taken together, our results may provide novel clues for early diagnosis of carotid restenosis and developing new therapeutic strategy.

Introduction

Carotid stenosis is a narrowing or constriction of the inner surface of the carotid artery [1–2]. It is a major risk factor for stroke that leads to brain damage [3–4]. Carotid stenosis is usually caused by atherosclerosis [5–6], characterized by the atherosclerostic plagues accumulating in the artery wall, thus occluding the blood flow. In the past decade, carotid angioplasty and stenting (CAS) has been developed into a credible alternative treatment to carotid endarterectomy (CEA) for the patients with symptomatic moderate- and high-grade stenosis [7–8]. However, restenosis still remains an unsolved issue following CAS treatment, and the restenosis rate is even higher than that following CEA treatment [9–10]. Restenosis is a healing process of the arterial wall in response to mechanical injury caused by CAS and comprises two major processes—neointimal hyperplasia and vessel remodeling [11–12]. Thus, to develop more effective therapeutic approaches for preventing restenosis, a better understanding of the molecular mechanism of restenosis is important.

One of important characters of restenosis is vascular smooth muscle cells (VSMC) proliferation, which is likely to be mediated by transforming growth factor-beta (TGF-β)/bone morphogenic protein (BMP) signaling pathway. TGF-β is known to play an important role in the development of restenosis [13–14]. As a profibrotic cytokine, TGF-β enhances neointimal formation by stimulating extracellular matrix (ECM) synthesis [15–16]. Among the many downstream effectors of TGF-β, Smad3 might play a critical role in the formation of intimal hyperplasia. It has been shown that TGF- β1, through Smad3-dependent pathway, stimulates VSMC fibronectin synthesis, thus enhancing neointimal ECM accumulation [17]. Moreover, it has been shown that Smad3 overexpression also enhanced intimal hyperplasia via the stimulation of VSMC proliferation [13,15]. Smad3 acts as a transcription factor that regulates the expression of a number of genes in the cellular processes [18–19]. However, how Smad3 regulates in the carotid artery restenosis is largely unknown.

Besides TGF-β, bone morphogenetic protein (BMP) is another important member of the TGF-β superfamily [20–21]. To date, around 20 BMP family members have been identified and characterized [22]. BMPs signal through serine/threonine kinase receptors, composed of type I and II subtypes [23]. Three type I receptors have been shown to bind BMP ligands, type IA and IB BMP receptors (BMPR1A and BMPR1B) and type IA activin receptor (ActR1A) [23]. Three type II receptors for BMPs have also been identified, including type II BMP receptor (BMPR2) and type II and IIB activin receptors (ActR2 and ActR2B) [23]. The function of BMP-depednent signaling pathway in carotid artery restenosis is not clear. However, loss-of-function mutations of BMP receptor II (BMPR2) are often identified in the patients with familial pulmonary arterial hypertension (PAH) [24–25], a disease characterized by excessive VSMC proliferation, particularly in response to TGF-β [26]. Thus, BMPR2 is likely to act as a negative regulator to suppress TGF-β-dependent VSMC proliferation. Since VSMC proliferation causes neointimal formation in carotid restenosis [11–12], we hypothesize that Smad3-dependent signaling pathway has functional crosstalk with BMPR2 in VSMC proliferation during carotid restenosis.

Using human tissue samples, we confirmed that Smad3 is significantly up-regulated in carotid restenosis. Moreover, we found that the expression level of BMPR2 was significantly reduced in human carotid artery restenosis. The ChIP analyses showed that BMPR2 was targeted by miR-17~92 cluster members, which is controlled by Smad3. Thus, our results reveal a novel crosstalk between Smad3 and BMPR2 through miR-17~92 cluster in carotid artery restenosis. This study may provide a novel therapeutic strategy for carotid artery restenosis, namely inhibition of miR-17~92 cluster, which may not only block the Smad3 function but also rescue BMPR2 expression to relieve the neointimal formation.

Material and methods

Sample collection

The carotid artery tissues were surgically removed from the patients with carotid restenosis following CAS treatment at Xuan Wu Hospital in China, and were harvested for this study in accordance with informed consent principles.

Peripheral blood samples were collected from the patients with carotid restenosis following CAS treatment at Xuanwu Hospital, Beijing, China after informed consent from the subjects. Peripheral blood samples from healthy volunteers were also collected as control. All the samples used in this study were harvested after obtaining approval from the ethics committees at Capital Medical University and University of Michigan.

Cell Culture

Human carotid Artery Smooth Muscle Cells (hHCtASMCs) were obtained commercially (Cell Applications, Inc) and cultured in Medium 231 supplemented with Smooth Muscle Growth Supplement (Life technologies) in 37 °C with 5 % CO2.

Histopathology and immunofluorescent staining

The artery specimens were fixed in 4 % paraformaldehyde for 24 hours, dehydrated, and embedded into paraffin wax, then sectioned to 4 μm slides and processed for H&E staining.

To perform immunofluorescent staining, sections were deparaffinized and incubated with rabbit anti-Smad3 (1:500) (Upstate, Cat. # 04-1035), mouse anti-BMPR2 (1:500) (Millipore, Cat. # MABD171). The slides were then washed in PBS and incubated with FITC-coupled secondary antibodies (1:1000, Jackson ImmunoResearch Laboratories).

Transfection of siRNAs to hHCtASMCs

hHCtASMCs were transfected with 500 ng of Smad3 siRNA and Mock siRNAs using a Primary P1 Nucleofection Kit and 4D Nucleofector machine (Lonza). Smad3 siRNA sequence was ordered from Dharmacon. The siRNA sense sequence is 5′-GAGAAACCAGUGACCACCA-3′. And the mock siRNA sense sequence is 5′-CUACAACUCCCACAACGUA-3′.

RNA isolation and quantitative reverse transcription polymerase chain reaction (qRT-PCR)

Total RNA was isolated from the carotid artery tissue using Trizol (Invitrogen) according to the manufacturer’s instruction. For circulating RNA isolation, whole blood was allowed to stand overnight at 4 °C, and total circulating RNA was isolated from 200 μl serum using miRNeasy mini kit according to the manufacturer’s protocol (Qiagen).

Approximately 500 ng of total RNA was reverse transcribed using oligo-dT primers. The cDNA was utilized as a template to amplify target genes with SYBR Premix Ex Tag Kit (TaKaRa). Specific primers were listed in Supplementary table 1. Each RNA sample was evaluated in triplicate. Gene expression results were analyzed with the ΔΔCt method and normalized to β-actin expression. The qRT-PCR assay was performed on Bio-Rad iQ5 instrument. The data were analyzed using Optical System Software 2.0.

The expression levels of mature miRNAs were determined using miRNA-specific qRT-PCR. The specific RT and PCR primers for miR-17, 18a, 19a, 20a, 92a, 130a, 21 and U6 were purchased from Qiagen. Reverse transcription of miRNAs was performed with a miScript Reverse Transcription Kit (QiAGEN). The qPCR was performed on Bio-Rad IQ 5 instrument by using SYBR Premix Ex Tag Kit (TaKaRa). The expression levels were normalized to the U6 endogenous control and measured by the comparative Ct (ΔΔCt) method.

Dual luciferase reporter gene construct and dual luciferase reporter assay

The target site of microRNA with ~60 flanking sequence in BMPR2 3′UTR was synthesized with cleavage sites for Xba I (5′ end) and Not I (3′ end), and a mutated sequence of the binding site was also synthesized. The two constructs were termed as WT and Mut. The DNA oligos were cloned into the pRL-TK vector (Promega). The DNA sequences containing the miR-17~92 cluster, miR-130a and miR-21 target sites as well as the mutant are shown in Supplementary Table 1. Each vector, along with 100 ng of PGL3 and 200 nmol/L miR-17~92 cluster, miR-130 and miR-21 or mimic control, were transfected into 293T cells. Cells were assayed for renilla and firefly luciferase activity using the Dual-Luciferase Reporter System (Promega).

Chromatin immunoprecipiatation assay

Chromatin immunoprecipation assays (ChIP) for artery tissues were performed according to the protocol described by Upstate. Briefly, 30 mg of fresh artery tissues per antibody in every ChIP were chopped into small pieces and transferred into 10 ml PBS (plus protease inhibitor). The artery tissues were fixed by 1 % formaldehyde at room temperature for 15 minutes, and then stopped by adding fresh glycine to a final concentration of 0.125 M at temperature for 5 minutes. After washed by cold PBS twice, the artery tissues were resuspended in 1 ml cold PBS (plus protease inhibitors). To achieve a single cell suspension, the Medimachine from Becton Dickinson was used to grind tissue for 2 minutes. After centrifugation, the cell pellet was suspended in 6 × volume of cell lysis buffer plus protease inhibitors for 15 minutes on ice, and lysed using a B dounce several times to aid in nuclei release. Cell nuclei were collected by centrifugation at 1000 g at 4 °C. The cell nuclei were resuspended in 5 × volume of nuclei lysis buffer plus the protease inhibitors, incubated on ice for 20 minutes and then subjected to sonication. The artery tissue DNA was sonicated to an average size between 300 and 600 bp. Solubilized chromatin was immunoprecipitated with antibody against Smad3 (Upstate). And IgG was used as control. Antibody-chromatin complexes were pulled-down using protein A-sepharose. After cross-link reversal and proteinase K treatment, immunoprecipated DNA was extracted with phenol-chloroform, ethanol precipitated, and treated with RNase. ChIP DNA was subjected to PCR amplification using primers flanking the Smad3 binding site at the miRNA gene promoter.

Protein Extraction and Western blotting

Protein samples were extracted from the artery tissues by using total protein extraction kit (Millipore). The protein concentration was determined using BCA standard curve. Equal amount of protein extract were separated by SDS-PAGE and transferred eletrophoretically to PVDF membranes (Millipore). The membranes were blocked in TBST containing 5 % milk att room temperature for 1 hour. After washing with TBST, the blocked membranes were probed with rabbit anti-Smad3 (1:500) (Upstate), mouse anti-BMPR2 (1:500) (Millipore) and mouse anti-β-actin (1:10,000) (Sigma) primary antibodies respectively overnight at 4 °C. After three consecutive 10-minute washes with TBST, the membrane was incubated with HRP-conjugated goat-anti-rabbit or goat-anti- mouse secondary antibody for 1 hour. The membrane was washed again three times with TBST and developed using the ECL+detection system (GE Healthcare).

Statistical analysis

All the experiments were performed at least three times. Two-tailed Student’s t-test was used for comparing two groups. ANOVA was used for multiple-group (> 2) comparison. Data were presented as mean ± S.D. p values less than 0.05 were considered statistically signi cant.

Result and Discussion

In the past decade, CAS has been proposed as a safe and effective alternative to CEA for patients with carotid stenosis [7–8]. However, restenosis remains a severe and unsolved issue following CAS treatment [9–10]. One option for these patients is to surgically remove the blocked part of the carotid artery together with stent, and use graft to replace the removed vessels [27]. We harvested the carotid artery samples following this type of surgery. H&E staining was performed and neointima hyperplasia was observed in the carotid artery with stenosis, but not in the adjacent normal artery tissue (Fig. 1A).

Fig. 1. Expression changes of Smad3 and BMPR2 in carotid restenosis.

(A) Carotid artery with restenosis was surgically removed from the patients and harvested for H&E staining. Thickened neointima was observed (dark arrows). Adjacent normal carotid artery was shown as the control. Scale bars: 400 μm. (B) Immunofluorescence staining was performed to examine the expression level of Smad3 and BMPR2 in the carotid artery tissues. Significant increase of Smad3 was observed in the carotid artery with restenosis. In contrast, BMPR2 is down-regulated in carotid restenosis. The adjacent normal artery was used as the control. * artery wall; # neointima. Scale bars: 200 μm. (C) The result of qRT-PCR shows the significant increase of Smad3 in carotid artery restenosis. Number 1, 2, 3, 4, 5, 6 and 7 represent the carotid tissue samples from different patient. Error bars indicate S.D. (n=6) ** p < 0.01. (D) Western blotting was used to detect the protein levels of Smad3 and BMPR2. Smad3 up-regulation and BMPR2 down-regulation were observed in carotid restenosis. β-actin was used as protein loading control. (E) Quantitative analysis of the Western blotting results was performed using ImageJ. Around 80 % decrease of BMPR2 was observed in carotid restenosis. Error bars indicate S.D. (n=6) ** p < 0.01. (F) The result of qRT-PCR shows a slight decrease of BMPR2 mRNA level in carotid restenosis. Error bars indicate S.D. (n=6) * p < 0.05.

TGF-β1/Smad3 signaling pathway has been shown to play a key role for promoting the neointimal formation during carotid restenosis, including VSMC proliferation, through experimental studies in the animal models [16,15]. Thus, we examined the Smad3 expression levels in human carotid artery tissue with restenosis by immunofluorescence staining. As shown in Fig. 1B, Smad3 was positively stained in the neointima hyperplasia but not normal carotid artery. Moreover, we confirmed the up-regulation of Smad3 using both RT-PCR and Western blotting (Fig. 1C and D). Taken together, these results suggest that the expression of Smad3 was significantly increased in the carotid restenosis.

Besides Smad3, BMPR2 also regulates VSMC proliferation. Thus, we questioned whether BMPR2 participates in developing carotid restenosis. Again, we examined the expression of BMPR2 in carotid restenosis. Both immunofluorescence staining and Western blotting results indicated a significant decrease of the protein level of BMPR2 in carotid tissues with restenosis (Fig. 1B, C). With the quantitative analysis of Western blotting results, we found a ~ 80 % decrease of BMPR2 in carotid restenosis (Fig. 1E). However, the result of qRT-PCR showed a slight decrease of BMPR2 mRNA level in artery tissues with restenosis compared with that in normal adjacent artery tissues (Fig. 1F).

This inconsistent alteration of the expression of BMPR2 in mRNA and protein levels indicated that the down-regulation of BMPR2 could be mediated by microRNAs, which post-transcripitionally down-regulate the target genes [28]. Thus, we then used online software, TargetScan, to predict the candidate microRNAs that potentially regulate the expression of BMPR2 [29]. Several microRNAs were shown to potentially target the 3′ UTR of BMPR2 (Fig. 2A, B). Interestingly, four of these candidate microRNAs, including miR-17, miR-19ab, miR-20a and miR-92a, belong to miR-17~92 cluster. Conserved target sites of these four microRNAs were found in human BMPR2 3′ UTR (Fig. 2A). Of note, miR-17 and miR-20a share the same potential target sites because these two microRNAs have the same seed sequence in the 5′ end (Fig. 2A). In addition, miR-130a and miR-21 are also found to potentially target BMPR2 among prediction results (Fig. 2B). Luciferase assays were performed to examine whether BMPR2 was directly regulated by these six microRNAs. The target site of each of these six microRNAs in BMPR2 3′ UTR was cloned into the downstream of renilla luciferase coding frame of pRL-TK vector (Promeg). Each microRNA targeting site mutant was also generated. The result showed that luciferase signal could be significantly down-regulated by miR-17, miR-19a, miR-20a or miR-92a, but not miR-130a or miR-21 (Fig. 2C). This result indicated that it is miR-17~92 cluster members, but not miR-130a and miR-21, directly targeting BMPR2. Both miR-130a and miR-21 have been shown to involve in the carotid restenosis by targeting Gax and PTEN respectively [30–33]. Both Gax and PTEN can inhibit the proliferation of VSMC [34–35]. Thus, miR-130a and miR-21 are up-regulated in the carotid restenosis and are likely to promote VSMC proliferation by down-regulating the expression levels of Gax and PTEN respectively.

Fig. 2. BMPR2 is regulated by miR-17~92 cluster.

(A) Targeting sites of four miR-17~92 cluster members in BMPR2 3′ UTR were predicted by TargetScan. (B) Targeting sites of miR-130a and miR-21 in BMPR2 3′ UTR were predicted by TargetScan. (C) Luciferase assays were performed to confirm that BMPR2 was the target gene of miR-17, miR-19a, miR-20a and miR-92a, but not miR-130a and miR-21. Error bars indicate S.D. (n=6) ** p < 0.01. (D) The result of qRT-PCR shows that miR-17~92 cluster members were significantly up-regulated in carotid artery restenosis. Error bars indicate S.D. (n=7) ** p < 0.01. (E) Circulating miR-17~92 cluster members increased in serum of the patients with carotid restenosis. Circulating RNAs were isolated from 8 patients’ serum, and qRT-PCR was performed to detect the microRNA expression levels. The circulating microRNAs in the healthy volunteers’ serum were detected as the control. ** p < 0.01.

We next detected the expression level of miR-17, miR-19a, miR-20a, miR-92a as well as another miR-17~92 family member, miR-18a in the carotid artery tissues with restenosis. All these microRNAs were found to be significantly up-regulated in carotid restenosis compared with that in adjacent normal artery (Fig. 2D). These results further support that miR-17~92 cluster members induce the carotid restenosis formation by suppressing the expression of BMPR2. Of note, as a member of miR-17~92 cluster, miR-18a was also up-regulated in carotid artery restenosis although little evidence indicate that miR-18a is involved in artery restenosis. It is possible that the up-regulation of miR-18a might be a byproduct of expressing miR-17~92 cluster in the carotid artery tissue with restenosis (Fig. 2D).

Recently, extracellular miRNAs have been found to circulate in the bloodstream and the circulating miRNAs are remarkably stable [36–38]. Although the precise cellular release mechanism of miRNAs remains elusive, some studies revealed that these circulating miRNAs can be delivered to recipient cells, where they can regulate the translation of target genes [39–40]. It raises the possibility that miRNAs can be probed in the blood and can serve as novel diagnostic markers [38,41]. Thus, as a potential clinical application for the early diagnosis of the restenosis, we examined the serum level of four circulating miR-17~92 cluster members. We isolated the serum microRNAs from 8 patients with carotid restenosis following CAS as well as 8 healthy volunteers. The qRT-PCR was performed to detect the circulating microRNA levels. The up-regulation of miR-17~92 cluster members, including miR-17, miR-19a, miR-20a and miR-92a, was clearly observed in the serum from the patients (Fig. 2E).

Two TGF-β superfamily signaling pathways were involved in the process of human carotid artery restenosis, in which Smad3 is up-regulated, and BMPR2 was down-regulated. We next asked whether these two signaling pathways have crosstalk in carotid restenosis. Smad3 is one of core transcription factors of TGF-β signaling pathway, and has been shown to directly bind to the promoter of miR-200b/a as a transcription activator in gastric cancer cells [42]. The Smad-binding elements have also been identified, and the consensus sequence is GTCTG [42]. Here, we hypothesize that Smad3 might regulates BMPR2 via the activation of miR-17~92 cluster during restenosis. We found that the Smad-binding elements existed in the promoter region of miR-17~92 cluster (Fig. 3A). ChIP-qRT-PCR was then performed and we found that Smad3 indeed stayed in the promoter region of miR-17~92 gene cluster but not in the promoter region of miR-20b and miR-92b (Fig. 3B). To demonstrate that Smad3 activates the transcription of miR-17~92 gene cluster and suppresses the expression of BMPR2, we knocked down Smad3 in human carotid artery smooth muscle cells by siRNA (Fig. 3C). Compared with control siRNA treatment, loss of Smad3 significantly suppressed the expression of miR-17~92 gene cluster (Fig. 3D). Moreover, lacking Smad3 induced the up-regulation of the expression level of BMPR2 (Fig. 3E).

Fig. 3. miR-17~92 cluster is regulated by Smad3.

(A) Smad-binding sequence, GTCTG, was found in the promoter region of miR-17~92 gene cluster. (B) ChIP-qPCR results suggested that the promoter region of miR-17~92 gene cluster was bound by Smad3. Carotid artery tissues with restenosis from 7 patients were used for ChIP-qPCR assay. A irrelevant IgG was used as control. Error bars indicate S.D. (n=7) ** p < 0.01 versus IgG. (C) hHCtASMCs were transfected with Smad3 and Mock siRNA respectively. And qRT-PCR was performed to detect the Smad3 mRNA level in hHCtASMCs. Smad3 siRNA can effectively decrease the Smad3 mRNA level. Error bars indicate S.D. (n=6) ** p < 0.01 versus Mock. (D) Knockdown Smad3 suppresses the transcription of miR-17~92 gene cluster. hHCtASMCs were treated with Smad3 siRNA. The qRT-PCR was performed to detect the microRNA expression level. Error bars indicate S.D. (n=6) ** p < 0.01. (E) Western blotting was performed to examine the protein level of BMPR2. Sinificant increase of BMPR2 was observed in hHCtASMCs treated with Smad3 siRNA but not in the cells treated with Mock siRNA. β-actin was used as protein loading control.

Taken together, our study demonstrates that miR-17~92 cluster mediates the crosstalk between the two important TGF-β superfamily signaling pathways, TGF-β1/Smad3 and BMP/BMPR2, to promote the VSMC proliferation and neointimal formation in the carotid artery restenosis (Fig. 4).

Fig. 4. A model shows the functional crosstalk between Smad3 and BMPR2 via miR-17~92 in carotid artery restenosis.

Smad3 is significantly up-regulated in the carotid artery with restenosis to promote VSMC proliferation. Up-regulation of Smad3 activates the transcription of miR-17~92 cluster. Then, miR-17~92 family members down-regulate BMPR2, an inhibitor of VSMC proliferation, to further promote VSMC proliferation and carotid artery restenosis.