. 2014 Oct 25;3:632. doi: 10.1186/2193-1801-3-632

Table 1.

Oligonucleotide primers used in real-time RT-PCR

Target gene	Primer sequence (5'-3')
GAPDH	S:-GCCAAGAGGGTCATCATCTC
GAPDH	A:-GGCCCGTCCACGGTCTTCT
MITF	S:-GGGATTGATGGATCCTGCTTTG
MITF	A:-GGCTGGACAGGAGTTGCTGA
TYR	S:-TTGGCAGATTGTCTGTAGCC
TYR	A:-AGGCATTGTGCATGCTGCTT
TRP-2	S:-ACACAACACTGGCTGGGCCT
TRP-2	A:-GAGATCTCTTTCCAGACACAAC
PCNA	S:-AGTCACATCGGAGATGCTGTTGTA
PCNA	A:-AGCTGAACTGGCTCATTCATCTCTA
Ki67	S:-AATCTCTGCTTCGGGTCTCCA
Ki67	A:-ACTCCGGTTTCAGACGACCAC

GAPDH Glyceraldehyde-3-phosphate dehydrogenase, MITF microphthalmia- associated transcription factor, TYR tyrosinase, TRP-2 tyrosinase-related protein 2, PCNA proliferating cell nuclear factor, S sense, A; antisense.