Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1978 Nov;22(2):322–327. doi: 10.1128/iai.22.2.322-327.1978

Formation of antibody to matrix protein in experimental human influenza A virus infections.

L Cretescu, A S Beare, G C Schild
PMCID: PMC422157  PMID: 730356

Abstract

Antibodies to type A influenza virus matrix protein (M) were assayed by single radial diffusion in 180 paired sera of volunteers challenged intranasally with live H3N2 viruses of varying degrees of virulence. Of these volunteers 20 had had severe clinical reactions (influenza-like); there had been 19 moderate reactions (lesser degrees of constitutional illness), and the remaining 141 reactions had been graded mild, very mild, or nil. Only 2 volunteers were shown to have antibodies to M in the pre-trial serum samples, and 11 developed anti-M rises after virus inoculation. Nine of the 11 had had severe reactions, and 2 had had moderate reactions. There was, therefore, a clear correlation between severity of clinical illness and anti-M antibody formation. In general, anti-M increases coincided with increases to the hemagglutinins and nucleoprotein, and with virus shedding. However, no anti-M antibody could be demonstrated in paired sera of 18 additional volunteers of whom 12 had developed severe reactions after the inoculation of virulent H0N1 and H1N1 influenza A viruses and of whom 12 had shown laboratory evidence of infection.

Full text

PDF
322

Images in this article

324Image
on p.324

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aymard-Henry M., Coleman M. T., Dowdle W. R., Laver W. G., Schild G. C., Webster R. G. Influenzavirus neuraminidase and neuraminidase-inhibition test procedures. Bull World Health Organ. 1973;48(2):199–202. [PMC free article] [PubMed] [Google Scholar]
  2. Beare A. S., Hall T. S. Recombinant influenza-A viruses as live vaccines for man. Report to the Medical Research Council's Committee on Influenza and other Respiratory Virus Vaccines. Lancet. 1971 Dec 11;2(7737):1271–1273. doi: 10.1016/s0140-6736(71)90597-6. [DOI] [PubMed] [Google Scholar]
  3. Beare A. S., Schild G. C., Craig J. W. Trials in man with live recombinants made from A/PR/8/34 (H0 N1) and wild H3 N2 influenza viruses. Lancet. 1975 Oct 18;2(7938):729–732. doi: 10.1016/s0140-6736(75)90720-5. [DOI] [PubMed] [Google Scholar]
  4. Biddison W. E., Doherty P. C., Webster R. G. Antibody to influenza virus matrix protein detects a common antigen on the surface of cells infected with type A influenza viruses. J Exp Med. 1977 Sep 1;146(3):690–697. doi: 10.1084/jem.146.3.690. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. McCahon D., Beare A. S., Stealey V. The production of live attenuated influenza A strains by recombination with A/Okuda/57 (H2N2). Postgrad Med J. 1976 Jun;52(608):389–394. doi: 10.1136/pgmj.52.608.389. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. McCahon D., Schild G. C. Segregation of antigenic and biological characteristics during influenza virus recombination. J Gen Virol. 1972 Apr;15(1):73–77. doi: 10.1099/0022-1317-15-1-73. [DOI] [PubMed] [Google Scholar]
  7. Mostow S. R., Schild G. C., Dowdle W. R., Wood R. J. Application of the single radial diffusion test for assay of antibody to influenza type A viruses. J Clin Microbiol. 1975 Dec;2(6):531–540. doi: 10.1128/jcm.2.6.531-540.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Murphy B. R., Chalhub E. G., Nusinoff S. R., Chanock R. M. Temperature-sensitive mutants of influenza virus. II. Attenuation of ts recombinants for man. J Infect Dis. 1972 Aug;126(2):170–178. doi: 10.1093/infdis/126.2.170. [DOI] [PubMed] [Google Scholar]
  9. Oxford J. S., Schild G. C. Immunological and physicochemical studies of influenza matrix (M) polypeptides. Virology. 1976 Oct 15;74(2):394–402. doi: 10.1016/0042-6822(76)90345-7. [DOI] [PubMed] [Google Scholar]
  10. SCHULMAN J. L., KILBOURNE E. D. INDUCTION OF PARTIAL SPECIFIC HETEROTYPIC IMMUNITY IN MICE BY A SINGLE INFECTION WITH INFLUENZA A VIRUS. J Bacteriol. 1965 Jan;89:170–174. doi: 10.1128/jb.89.1.170-174.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Schild G. C. Evidence for a new type-specific structural antigen of the influenza virus particle. J Gen Virol. 1972 Apr;15(1):99–103. doi: 10.1099/0022-1317-15-1-99. [DOI] [PubMed] [Google Scholar]
  12. Schild G. C., Henry-Aymard M., Pereira H. G. A quantitative, single-radial-diffusion test for immunological studies with influenza virus. J Gen Virol. 1972 Aug;16(2):231–236. doi: 10.1099/0022-1317-16-2-231. [DOI] [PubMed] [Google Scholar]
  13. Waldman R. H., Spencer C. S., Johnson J. E., 3rd Respiratory and systemic cellular and humoral immune responses to influenza virus vaccine administered parenterally or by nose drops. Cell Immunol. 1972 Feb;3(2):294–300. doi: 10.1016/0008-8749(72)90168-2. [DOI] [PubMed] [Google Scholar]
  14. Webster R. G., Hinshaw V. S. Matrix protein from influenza A virus and its role in cross-protection in mice. Infect Immun. 1977 Sep;17(3):561–566. doi: 10.1128/iai.17.3.561-566.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES