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. 2014 Oct 6;3:e03502. doi: 10.7554/eLife.03502

Figure 3. Trx and the core components of the SET/MLL complex maintain a type II neurobalst functional identity dependently on their catalytic activity for H3K4 methylaiton.

(A–B) The function of trx for the H3K4 methylation is required for the maintenance of a type II neuroblast functional identity. (A–B) In the 72-hr clones, a trxZ11 mutant type II neuroblast displays a type I neuroblast marker expression profile and directly generates GMCs. Scale bar, 10 μm. Three-dimensionally reconstructed images of the clones are shown to the right. Scale bar, 10 μm. (C–K) The function of rbbp5 for the H3K4 methylation is required for the maintenance of a type II neuroblast functional identity. (C–E, H, J) In the 96-hr clones, rbbp5null type II neuroblasts display a type I neuroblast marker expression profile and directly generate GMCs. Over-expression of rbbp5FL but not rbbp5SG restores a type II neuroblast functional identity in rbbp5null type II neuroblasts. Three-dimensionally reconstructed images of the clones are shown to the right. Scale bar, 10 μm. (F) The frequency of type II neuroblasts of the indicated genotypes displaying the type I or type II marker expression profiles. (G, I, K) rbbp5 function is essential for the H3K4 methylation in fly larval brains. Scale bar, 10 μm.

DOI: http://dx.doi.org/10.7554/eLife.03502.007

Figure 3.

Figure 3—figure supplement 1. Decreasing the function of the core components of the SET1/MLL complex leads to a reduction in type II neuroblasts.

Figure 3—figure supplement 1.

(A–E) Knocking down the function of trx, rbbp5, wds or ash2 specifically reduces the number of type II neuroblasts per brain lobe. Scale bar, 20 μm. (F–G) The average number of type II neuroblasts or INPs per brain lobe of the indicated genotypes after knocking down the function of trx, rbbp5, wds, or ash2 for 72 hr.
DOI: http://dx.doi.org/10.7554/eLife.03502.008
Figure 3—figure supplement 2. Generation of the rbbp5null allele and the UAS-rbbp5SG transgene.

Figure 3—figure supplement 2.

(A) The genomic organization of the rbbp5 locus. The rbbp5null allele was generated via imprecise excision of the P(EP)G4226 element, which removes the entire rbbp5 coding region. Yellow squares indicate the coding exons of rbbp5 while blue squares indicate the untranslated regions. The red line indicates the molecular lesion induced by the rbbp5null allele. (B) The average number of INPs per clone of the indicated genotypes at 96 hr after clone induction. (C) An alignment of the hinge region of the yeast, fly, and human Rbbp5 protein. The amino acid substitutions in the Rbbp5SG transgenic protein are indicated in red.
DOI: http://dx.doi.org/10.7554/eLife.03502.009