Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1978 Nov;22(2):492–501. doi: 10.1128/iai.22.2.492-501.1978

Antibody-Dependent Cell-Mediated Cytotoxicity in Cattle: Activity Against 51Cr-Labeled Chicken Erythrocytes Coated with Protozoal Antigens

W P H Duffus †, A E Butterworth 1, G G Wagner 2, J M Preston 3, D Franks 4
PMCID: PMC422183  PMID: 103832

Abstract

Bovine mononuclear cells in the presence of bovine anti-chicken erythrocyte sera at high dilutions induce release of chromium-51 from labeled chicken erythrocytes. Bovine effector cells are capable of recognizing both bovine immunoglobulin G1 and bovine immunoglobulin G2; in contrast, human effector cells only recognize immunoglobulin G1. Effector cell activity of bovine mononuclear cells is equally distributed between peripheral blood and spleen. As in other species, thymus and lymph node cells exert no antibody-dependent effect, although some direct cytotoxicity by lymph node cells may be observed. Antibody-dependent cell-mediated cytotoxicity against a bovine cell line can also be detected. By using a tannic acid technique, it was found that chicken erythrocytes coated with Theileria parva piroplasm antigen or with Trypanosoma rhodesiense variant-specific coat antigen form suitable targets for bovine antibody-dependent cell-mediated cytotoxicity assays. By using such targets, a moderate degree of direct cytotoxicity by bovine mononuclear cells, in the absence of antibody, is always observed; this may be reduced by choosing optimal conditions of tannic acid treatment and antigen sensitization and by the use of short incubation periods for the cytotoxicity assay. Observations have been made on the variant specificity, time course of appearance, and association with immunoglobulin G1 of the antibody activity responsible for cell-dependent cytotoxicity against chicken erythrocytes coated with T. rhodesiense antigens. The potential usefulness of this technique in the analysis of protective immune responses against protozoal infections is discussed.

Full text

PDF
492

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blakeslee D., Butler J. E., Stone W. H. Serum antigens of cattle. II. Immunogenetics of two immunoglobulin allotypes. J Immunol. 1971 Jul;107(1):227–235. [PubMed] [Google Scholar]
  2. Butterworth A. E., Coombs R. R., Gurner B. W., Wilson A. B. Receptors for antibody-opsonic adherence on the eosinophils of guinea pigs. Int Arch Allergy Appl Immunol. 1976;51(3):368–377. doi: 10.1159/000231610. [DOI] [PubMed] [Google Scholar]
  3. Butterworth A. E., Sturrock R. F., Houba V., Mahmoud A. A., Sher A., Rees P. H. Eosinophils as mediators of antibody-dependent damage to schistosomula. Nature. 1975 Aug 28;256(5520):727–729. doi: 10.1038/256727a0. [DOI] [PubMed] [Google Scholar]
  4. Calder E. A., Penhale W. J., McLeman D., Barnes E. W., Irvine W. J. Lymphocyte-dependent antibody-mediated cytotoxicity in Hashimoto thyroiditis. Clin Exp Immunol. 1973 Jun;14(2):153–158. [PMC free article] [PubMed] [Google Scholar]
  5. Cerottini J. C., Brunner K. T. Cell-mediated cytotoxicity, allograft rejection, and tumor immunity. Adv Immunol. 1974;18:67–132. doi: 10.1016/s0065-2776(08)60308-9. [DOI] [PubMed] [Google Scholar]
  6. Cordier G., Samarut C., Brochier J., Revillard J. P. Antibody-dependent cell cytoxicity (ADCC). Characterization of 'killer' cells in human lymphoid organs. Scand J Immunol. 1976;5(3):233–242. doi: 10.1111/j.1365-3083.1976.tb00274.x. [DOI] [PubMed] [Google Scholar]
  7. Cross G. A. Identification, purification and properties of clone-specific glycoprotein antigens constituting the surface coat of Trypanosoma brucei. Parasitology. 1975 Dec;71(3):393–417. doi: 10.1017/s003118200004717x. [DOI] [PubMed] [Google Scholar]
  8. Denk H., Stemberger H., Wiedermann G., Eckerstorfer R., Tappeiner G. The influence of 2-mercaptoethanol (2-ME) treatment of IgG antibody on its ability to induce cytotoxicity in nonsensitized lymphocytes. Cell Immunol. 1974 Sep;13(3):489–492. doi: 10.1016/0008-8749(74)90268-8. [DOI] [PubMed] [Google Scholar]
  9. Duffus W. P., Wagner G. G. The specific immunoglobulin response in cattle immunized with Theileria parva (Muguga) stabilate. Parasitology. 1974 Aug;69(1):31–41. doi: 10.1017/s0031182000046151. [DOI] [PubMed] [Google Scholar]
  10. Franks D., Kelly M. M., Sanderson C. J. Inhibition of xenogeneic cell-mediated cytotoxicity in the rat by antiglobulin sera. Int Arch Allergy Appl Immunol. 1975;48(5):621–631. doi: 10.1159/000231350. [DOI] [PubMed] [Google Scholar]
  11. Grant C. K., Adams E., Miller H. R. Leukocyte-dependent antibody in sheep immunized with murine mastocytoma cells. Eur J Immunol. 1975 May;5(5):324–330. doi: 10.1002/eji.1830050507. [DOI] [PubMed] [Google Scholar]
  12. Lanham S. M., Godfrey D. G. Isolation of salivarian trypanosomes from man and other mammals using DEAE-cellulose. Exp Parasitol. 1970 Dec;28(3):521–534. doi: 10.1016/0014-4894(70)90120-7. [DOI] [PubMed] [Google Scholar]
  13. Lazáry S., Rivera E., de Weck A. L., Gerber H., Nicolet J. In vitro stimulation of bovine leucocytes by phytohaemagglutinin and other mitogens. Res Vet Sci. 1974 Nov;17(3):344–350. [PubMed] [Google Scholar]
  14. Lustig H. J., Bianco C. Antibody-mediated cell cytotoxicity in a defined system: regulation by antigen, antibody, and complement. J Immunol. 1976 Jan;116(1):253–260. [PubMed] [Google Scholar]
  15. MacLennan I. C. Antibody in the induction and inhibition of lymphocyte cytotoxicity. Transplant Rev. 1972;13:67–90. doi: 10.1111/j.1600-065x.1972.tb00060.x. [DOI] [PubMed] [Google Scholar]
  16. Mkwananzi J. B., Franks D., Baker J. R. Cytotoxicity of antibody-coated trypanosomes by normal human lymphoid cells. Nature. 1976 Feb 5;259(5542):403–404. doi: 10.1038/259403a0. [DOI] [PubMed] [Google Scholar]
  17. Muhammed S. I., Wagner G. G., Lauerman L. H., Jr Leucocyte migration inhibition as a model for the demonstration of sensitized cells in East Coast fever. Immunology. 1974 Dec;27(6):1033–1037. [PMC free article] [PubMed] [Google Scholar]
  18. Muscoplat C. C., Chen A. W., Johnson D. W., Alhaji I. In vitro stimulation of bovine peripheral blood lymphocytes: standardization and kinetics of the response. Am J Vet Res. 1974 Dec;35(12):1557–1561. [PubMed] [Google Scholar]
  19. Plackett P., Alton G. G. A mechanism for prozone formation in the complement fixation test for bovine brucellosis. Aust Vet J. 1975 Aug;51(8):374–377. doi: 10.1111/j.1751-0813.1975.tb15598.x. [DOI] [PubMed] [Google Scholar]
  20. Rouse B. T., Wardley R. C., Babiuk L. A. Antibody-dependent cell-mediated cytotoxicity in cows: comparison of effector cell activity against heterologous erthrocyte and herpesvirus-infected bovine target cells. Infect Immun. 1976 May;13(5):1433–1441. doi: 10.1128/iai.13.5.1433-1441.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rouse B. T., Wardley R. C., Babiuk L. A. The role of antibody dependent cytotoxicity in recovery from herpesvirus infections. Cell Immunol. 1976 Mar 1;22(1):182–186. doi: 10.1016/0008-8749(76)90019-8. [DOI] [PubMed] [Google Scholar]
  22. Schirrmacher V., Golstein P. Cytotoxic immune cells with specificity for defined soluble antigens. I. Assay with antigen-coated target cells. Cell Immunol. 1973 Nov;9(2):198–210. doi: 10.1016/0008-8749(73)90071-3. [DOI] [PubMed] [Google Scholar]
  23. Torten M., Johnson R. C., Kaattari S., Leung C., Benjamini E. Immune plasma-dependent cytotoxicity of immune and non-immune peripheral lymphoid cells for target cells coated with bacterial outer unit membrane. Immunology. 1975 Dec;29(6):1093–1102. [PMC free article] [PubMed] [Google Scholar]
  24. Wagner G. G., Duffus W. P., Akwabi C., Burridge M. J., Lule M. The specific immunoglobulin response in cattle to Theileria parva (Muguga) infection. Parasitology. 1975 Feb;70(1):95–102. doi: 10.1017/s0031182000048903. [DOI] [PubMed] [Google Scholar]
  25. Wagner G. G., Duffus W. P., Kimber C. D., Lule M. Immunochemical studies on East Coast fever. II. Partial segregation and characterization of the Theileria parva piroplasm antigen. J Parasitol. 1974 Oct;60(5):854–859. [PubMed] [Google Scholar]
  26. Wardley R. C., Rouse B. T., Babiuk L. A. Lymphocyte activation by cell separation procedures. Immunol Commun. 1976;5(7-8):637–648. doi: 10.3109/08820137609033872. [DOI] [PubMed] [Google Scholar]
  27. Wasserman J., Packalén T., Perlmann P., Perlmann H. Cytotoxic lymphoid cells and antibodies from guinea pigs immunized with tubercle bacilli. Int Arch Allergy Appl Immunol. 1969;36(1):115–116. doi: 10.1159/000230730. [DOI] [PubMed] [Google Scholar]
  28. Wasserman J., von Stedingk L. V., Perlmann P., Jonsson J. Antibody-induced in vitro lymphocyte cytotoxicity in Hashimoto thyroiditis. Int Arch Allergy Appl Immunol. 1974;47(4):473–482. doi: 10.1159/000231241. [DOI] [PubMed] [Google Scholar]
  29. Wellde B. T., Duxbury R. E., Sadun E. H., Langbehn H. R., Lötzsch R., Deindl G., Warui G. Experimental infections with African trypanosomes. IV. Immunization of cattle with gamma-irradiated Trypanosoma rhodesiense. Exp Parasitol. 1973 Aug;34(1):62–68. doi: 10.1016/0014-4894(73)90063-5. [DOI] [PubMed] [Google Scholar]
  30. Wiedermann G., Denk H., Stemberger H., Eckerstorfer R., Tappeiner G. Influence of the antigenicity of target cells on the antibody-mediated cytotoxicity of nonsensitized lymphocytes. Cell Immunol. 1975 Jun;17(2):440–446. doi: 10.1016/s0008-8749(75)80048-7. [DOI] [PubMed] [Google Scholar]
  31. Zighelboim J., Gale R. P. Interspecies variability in antibody-dependent cellular cytotoxicity (ADCC). J Immunol. 1974 Oct;113(4):1145–1150. [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES