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. 1978 Dec;22(3):676–680. doi: 10.1128/iai.22.3.676-680.1978

Heat-labile B-cell mitogen obtained from Listeria monocytogenes.

R J Kearns, D J Hinrichs
PMCID: PMC422213  PMID: 103837

Abstract

A water-soluble extract of Listeria monocytogenes strain 10403 acts as a mitogen on cultured mouse spleen lymphocytes. This mitogen induced a response six to nine times that of controls, as measured by [3H]thymidine incorporation. The mitogen extract was derived from washed bacterial cells which were mechanically disrupted with a French press. The extract was centrifuged at 105,000 X g and filtered through a 0.22-micrometer filter. Similar levels of lymphocyte stimulation were observed in lymphocyte cultures prepared from spleens of nude mice, indicating the effect of this mitogen on B-cells. The mitogenic property of this extract was destroyed by heating to 56 degrees C. This heat treatment does not destroy the antigens in the extract, which stimulate spleen cell cultures obtained from specifically immune mice. Similarly prepared extracts from Staphylococcus epidermidis and Salmonella typhimurium did not show similar levels of mitogenic activity. The mitogenic property of the L. monocytogenes extract was present in two strains of Listeria tested and was not associated with mouse virulence.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersson J., Melchers F., Galanos C., Lüderitz O. The mitogenic effect of lipopolysaccharide on bone marrow-derived mouse lymphocytes. Lipid A as the mitogenic part of the molecule. J Exp Med. 1973 Apr 1;137(4):943–953. doi: 10.1084/jem.137.4.943. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baker L. A., Campbell P. A., Hollister J. R. Chemotaxigenesis and complement fixation by Listeria monocytogenes cell wall fractions. J Immunol. 1977 Nov;119(5):1723–1726. [PubMed] [Google Scholar]
  3. Bona C., Damais C., Chedid L. Blastic transformtion of mouse spleen lymphocytes by a water-soluble mitogen extracted from Nocardia. Proc Natl Acad Sci U S A. 1974 May;71(5):1602–1606. doi: 10.1073/pnas.71.5.1602. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Campbell P. A., Schuffler C., Rodriguez G. E. Listeria cell wall fraction: a B cell adjuvant. J Immunol. 1976 Mar;116(3):590–594. [PubMed] [Google Scholar]
  5. Ciorbaru R., Petit J. F., Lederer E., Zissman E., Bona C., Chedid L. Presence and subcellular localization of two distinct mitogenic fractions in the cells of Nocardia rubra and Nocardia opaca: preparation of soluble mitogenic peptidoglycan fractions. Infect Immun. 1976 Apr;13(4):1084–1090. doi: 10.1128/iai.13.4.1084-1090.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cohen J. J., Rodriguez G. E., Kind P. D., Campbell P. A. Listeria cell wall fraction: a B cell mitogen. J Immunol. 1975 Mar;114(3):1132–1134. [PubMed] [Google Scholar]
  7. Damais C., Bona C., Chedid L., Fleck J., Nauciel C., Martin J. P. Mitogenic effect of bacterial peptidoglycans possessing adjuvant activity. J Immunol. 1975 Jul;115(1):268–271. [PubMed] [Google Scholar]
  8. Engel D., Clagett J., Page R., Williams B. Mitogenic activity of Actinomyces viscosus. I. Effects on murine B and T lymphocytes, and partial characterization. J Immunol. 1977 Apr;118(4):1466–1471. [PubMed] [Google Scholar]
  9. Gery I., Krüger J., Spiesel S. Z. Stimulation of B-lymphocytes by endotoxin. Reactions of thymus-deprived mice and karyotypic analysis of dividing cells in mice bearing T 6 T 6 thymus grafts. J Immunol. 1972 Apr;108(4):1088–1091. [PubMed] [Google Scholar]
  10. Groves W. E., Davis F. C., Jr, Sells B. H. Spectrophotometric determination of microgram quantities of protein without nucleic acid interference. Anal Biochem. 1968 Feb;22(2):195–210. doi: 10.1016/0003-2697(68)90307-2. [DOI] [PubMed] [Google Scholar]
  11. Kearns R. J., Hinrichs D. J. Kinetics and maintenance of acquired resistance in mice to Listeria monocytogenes. Infect Immun. 1977 Jun;16(3):923–927. doi: 10.1128/iai.16.3.923-927.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Melchers F., Braun V., Galanos C. The lipoprotein of the outer membrane of Escherichia coli: a B-lymphocyte mitogen. J Exp Med. 1975 Aug 1;142(2):473–482. doi: 10.1084/jem.142.2.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mishell R. I., Dutton R. W. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967 Sep 1;126(3):423–442. doi: 10.1084/jem.126.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Petit J. C., Unanue E. R. Effects of bacterial products on lymphocytes and macrophages: their possible role in natural resistance to listeria infetion in mice. J Immunol. 1974 Sep;113(3):984–992. [PubMed] [Google Scholar]
  15. Rodriguez G. E., McClatchy J. K., Campbell P. A. Induction of Resistance by Listeria monocytogenes Cell Wall Fraction. Infect Immun. 1974 Nov;10(5):1163–1169. doi: 10.1128/iai.10.5.1163-1169.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sultzer B. M., Nilsson B. S. PPD tuberculin--a B-cell mitogen. Nat New Biol. 1972 Dec 13;240(102):198–200. doi: 10.1038/newbio240198a0. [DOI] [PubMed] [Google Scholar]
  17. Yin E. T., Galanos C., Kinsky S., Bradshaw R. A., Wessler S., Lüderitz O., Sarmiento M. E. Picogram-sensitive assay for endotoxin: gelation of Limulus polyphemus blood cell lysate induced by purified lipopolysaccharides and lipid A from Gram-negative bacteria. Biochim Biophys Acta. 1972 Jan 28;261(1):284–289. doi: 10.1016/0304-4165(72)90340-6. [DOI] [PubMed] [Google Scholar]

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