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. 1978 Dec;22(3):689–697. doi: 10.1128/iai.22.3.689-697.1978

Influence of oxygen tension, sulfhydryl compounds, and serum on the motility and virulence of Treponema pallidum (Nichols strain) in a cell-free system.

S J Norris, J N Miller, J A Sykes, T J Fitzgerald
PMCID: PMC422215  PMID: 365765

Abstract

The motility and virulence of Treponema pallidum (Nichols strain) were monitored during incubation in a modified tissue culture medium to study the effects of oxygen tension and medium composition on survival of the organism. A basal medium of Eagle minimal essential medium with 50% fresh, heat-inactivated normal rabbit serum was used inasmuch as better survival occurred with 50% normal rabbit serum than with lower concentrations. Addition of 0.5 to 2.0 mM dithiothreitol or 2.0 mM dithioerythritol to the basal medium led to significantly longer retention of T. pallidum viability in the presence of 3% oxygen than under aerobic or anaerobic conditions. The results of this investigation lend support to the classification of T. pallidum as a microaerophilic organism and provide direction for the design of potentially successful culture systems, with or without tissue culture cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baseman J. B., Nichols J. C., Hayes N. C. Virulent Treponema pallidum: aerobe or anaerobe. Infect Immun. 1976 Mar;13(3):704–711. doi: 10.1128/iai.13.3.704-711.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CLARK J. W., Jr Loss of virulence in vitro of motile Treponema pallidum. Br J Vener Dis. 1962 Jun;38:78–81. doi: 10.1136/sti.38.2.78. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CLELAND W. W. DITHIOTHREITOL, A NEW PROTECTIVE REAGENT FOR SH GROUPS. Biochemistry. 1964 Apr;3:480–482. doi: 10.1021/bi00892a002. [DOI] [PubMed] [Google Scholar]
  4. Cox C. D., Barber M. K. Oxygen uptake by Treponema pallidum. Infect Immun. 1974 Jul;10(1):123–127. doi: 10.1128/iai.10.1.123-127.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fieldsteel A. H., Becker F. A., Stout J. G. Prolonged survival of virulent Treponema pallidum (Nichols strain) in cell-free and tissue culture systems. Infect Immun. 1977 Oct;18(1):173–182. doi: 10.1128/iai.18.1.173-182.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fitzgerald T. J., Johnson R. C., Sykes J. A., Miller J. N. Interaction of Treponema pallidum (Nichols strain) with cultured mammalian cells: effects of oxygen, reducing agents, serum supplements, and different cell types. Infect Immun. 1977 Feb;15(2):444–452. doi: 10.1128/iai.15.2.444-452.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fitzgerald T. J., Miller J. N., Sykes J. A. Treponema pallidum (Nichols strain) in tissue cultures: cellular attachment, entry, and survival. Infect Immun. 1975 May;11(5):1133–1140. doi: 10.1128/iai.11.5.1133-1140.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Foster J. W., Kellogg D. S., Clark J. W., Balows A. The in vitro cultivation of Treponema pallidum. Corroborative studies. Br J Vener Dis. 1977 Dec;53(6):338–339. doi: 10.1136/sti.53.6.338. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Graves S. R., Sandok P. L., Jenkin H. M., Johnson R. C. Retention of motility and virulence of Treponema pallidum (Nichols strain) in vitro. Infect Immun. 1975 Nov;12(5):1116–1120. doi: 10.1128/iai.12.5.1116-1120.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Haugaard N. Cellular mechanisms of oxygen toxicity. Physiol Rev. 1968 Apr;48(2):311–373. doi: 10.1152/physrev.1968.48.2.311. [DOI] [PubMed] [Google Scholar]
  11. Jones R. H., Finn M. A., Thomas J. J., Folger C. Growth and subculture of pathogenic T. pallidum (Nichols strain) in BHK-21 cultured tissue cells. Br J Vener Dis. 1976 Feb;52(1):18–23. doi: 10.1136/sti.52.1.18. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. KIMM G. E., ALLEN R. H., MORTON H. J., MORGAN J. F. Enhancement of survival in vitro of Treponema pallidum by addition of glucose and magnesium. J Bacteriol. 1960 Nov;80:726–727. doi: 10.1128/jb.80.5.726-727.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. KIMM G. E., ALLEN R. H., MORTON H. J., MORGAN J. F. Studies on the in vitro survival of virulent Treponema pallidum. I. Methodology and basal synthetic medium. Am J Hyg. 1962 May;75:339–346. doi: 10.1093/oxfordjournals.aje.a120255. [DOI] [PubMed] [Google Scholar]
  14. Lysko P. G., Cox C. D. Terminal electron transport in Treponema pallidum. Infect Immun. 1977 Jun;16(3):885–890. doi: 10.1128/iai.16.3.885-890.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Miao R., Fieldsteel A. H. Genetics of Treponema: relationship between Treponema pallidum and five cultivable treponemes. J Bacteriol. 1978 Jan;133(1):101–107. doi: 10.1128/jb.133.1.101-107.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Nichols J. C., Baseman J. B. Ribosomal ribonucleic acid synthesis by virulent Treponema pallidum. Infect Immun. 1978 Mar;19(3):854–860. doi: 10.1128/iai.19.3.854-860.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. RICE F. A. H., NELSON R. A., Jr The isolation from beef serum of a survival factor for Treponema pallidum. J Biol Chem. 1951 Jul;191(1):35–41. [PubMed] [Google Scholar]
  18. Rathlev T. Investigations on in vitro survival and virulence of T. pallidum under aerobiosis. Br J Vener Dis. 1975 Oct;51(5):296–300. doi: 10.1136/sti.51.5.296. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. SYKES J. A., MOORE E. B. A simple tissue culture chamber. Tex Rep Biol Med. 1960;18:288–297. [PubMed] [Google Scholar]
  20. Sandok P. L., Jenkin H. M., Graves S. R., Knight S. T. Retention of motility of Treponema pallidum (Nichols virulent strain) in an anaerobic cell culture system and in a cell-free system. J Clin Microbiol. 1976 Jan;3(1):72–74. doi: 10.1128/jcm.3.1.72-74.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sandok P. L., Jenkin H. M., Matthews H. M., Roberts M. S. Unsustained multiplication of treponema pallidum (nichols virulent strain) in vitro in the presence of oxygen. Infect Immun. 1978 Feb;19(2):421–429. doi: 10.1128/iai.19.2.421-429.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sandok P. L., Knight S. T., Jenkin H. M. Examination of various cell culture techniques for co-incubation of virulent Treponema pallidum (Nichols I strain) under anaerobic conditions. J Clin Microbiol. 1976 Oct;4(4):360–371. doi: 10.1128/jcm.4.4.360-371.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. WEBER M. M. Factors influencing the in vitro survival of Treponema pallidum. Am J Hyg. 1960 May;71:401–417. doi: 10.1093/oxfordjournals.aje.a120123. [DOI] [PubMed] [Google Scholar]
  24. Willcox R. R., Guthe T. Treponema pallidum. A bibliographical review of the morphology, culture and survival of T. pallidum and associated organisms. Bull World Health Organ. 1966;35:1–169. [PMC free article] [PubMed] [Google Scholar]
  25. Yost F. J., Jr, Fridovich I. Superoxide and hydrogen peroxide in oxygen damage. Arch Biochem Biophys. 1976 Aug;175(2):514–519. doi: 10.1016/0003-9861(76)90539-7. [DOI] [PubMed] [Google Scholar]

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