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. 1978 Dec;22(3):758–762. doi: 10.1128/iai.22.3.758-762.1978

Host defense mechanisms against influenza virus: interaction of influenza virus with murine macrophages in vitro.

M A Wells, P Albrecht, S Daniel, F A Ennis
PMCID: PMC422225  PMID: 569645

Abstract

The interaction of mouse macrophages with influenza virus was examined as part of a study into the defense mechanisms against influenza infection. Macrophages exposed to A/Port Chalmers/1/73 virus produced infectious foci on susceptible indicator cell monolayers. Sampling of supernatant fluids and cells from infected macrophage cultures showed release of virus adsorbed to the cell surface. Active virus replication in macrophages could not be demonstrated. Exposing macrophages to specific antibody before or after virus infection resulted in a significant decrease in the number of infectious macrophages. The results suggest that although macrophages are not the source of replicating influenza virus, they are able to spread the infection by having virus attaching to their surface. This activity is interfered with by the presence of specific antibody.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berken A., Benacerraf B. Properties of antibodies cytophilic for macrophages. J Exp Med. 1966 Jan 1;123(1):119–144. doi: 10.1084/jem.123.1.119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ennis F. A., Martin W. J., Verbonitz M. W., Butchko G. M. Specificity studies on cytotoxic thymus-derived lymphocytes reactive with influenza virus-infected cells: evidence for dual recognition of H-2 and viral hemagglutinin antigens. Proc Natl Acad Sci U S A. 1977 Jul;74(7):3006–3010. doi: 10.1073/pnas.74.7.3006. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ennis F. A., Martin W. J., Verbonitz M. W. Cytotoxic T lymphocytes induced in mice by inactivated influenza virus vaccine. Nature. 1977 Sep 29;269(5627):418–419. doi: 10.1038/269418a0. [DOI] [PubMed] [Google Scholar]
  4. Ennis F. A., Martin W. J., Verbonitz M. W. Hemagglutinin-specific cytotoxic T-cell response during influenza infection. J Exp Med. 1977 Sep 1;146(3):893–898. doi: 10.1084/jem.146.3.893. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ennis F. A., Ruth W. A., Wells M. A. Host defense mechanisms against infection with influenza virus. I. Effect of sensitized spleen cells on infection in vitro. J Infect Dis. 1974 Sep;130(3):248–256. doi: 10.1093/infdis/130.3.248. [DOI] [PubMed] [Google Scholar]
  6. Ennis F. A., Wells M. A., Barry D. W., Daniel S., Manischewitz J. Host defence mechanisms against influenza infection. II. Protection of mice with vaccines against A/Port Chalmers/1/73 and B/Hong Kong/5/72. Postgrad Med J. 1976 Jun;52(608):338–344. doi: 10.1136/pgmj.52.608.338. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lindenmann J., Deuel E., Fanconi S., Haller O. Inborn resistance of mice to myxoviruses: macrophages express phenotype in vitro. J Exp Med. 1978 Feb 1;147(2):531–540. doi: 10.1084/jem.147.2.531. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. MIMS C. A. ASPECTS OF THE PATHOGENESIS OF VIRUS DISEASES. Bacteriol Rev. 1964 Mar;28:30–71. doi: 10.1128/br.28.1.30-71.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MIMS C. A. THE PERITONEAL MACROPHAGES OF MICE. Br J Exp Pathol. 1964 Feb;45:37–43. [PMC free article] [PubMed] [Google Scholar]
  10. ROBERTS J. A. GROWTH OF VIRULENT AND ATTENUATED ECTROMELIA VIRUS IN CULTURED MACROPHAGES FROM NORMAL AND ECTROMELIAIMMUNE MICE. J Immunol. 1964 Jun;92:837–842. [PubMed] [Google Scholar]
  11. Sawyer W. D. Interaction of influenza virus with leukocytes and its effect on phagocytosis. J Infect Dis. 1969 Jun;119(6):541–556. doi: 10.1093/infdis/119.6.541. [DOI] [PubMed] [Google Scholar]
  12. Shayegani M., Lief F. S., Mudd S. Specific and nonspecific cell-mediated resistance to influenza virus in mice. Infect Immun. 1974 Jun;9(6):991–998. doi: 10.1128/iai.9.6.991-998.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Silverstein S. Macrophages and viral immunity. Semin Hematol. 1970 Apr;7(2):185–214. [PubMed] [Google Scholar]
  14. Unanue E. R., Askonas B. A. Persistence of immunogenicity of antigen after uptake by macrophages. J Exp Med. 1968 May 1;127(5):915–926. doi: 10.1084/jem.127.5.915. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Unanue E. R., Cerottini J. C. The function of macrophages in the immune response. Semin Hematol. 1970 Apr;7(2):225–248. [PubMed] [Google Scholar]
  16. Verbonitz M. W., Ennis F. A., Hicks J. T., Albrecht P. Hemagglutinin-specific complement-dependent cytolytic antibody response to influenza infection. J Exp Med. 1978 Jan 1;147(1):265–270. doi: 10.1084/jem.147.1.265. [DOI] [PMC free article] [PubMed] [Google Scholar]

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