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. 1978 Dec;22(3):798–803. doi: 10.1128/iai.22.3.798-803.1978

Host defenses in murine malaria: induction of a protracted state of immunity with a formalin-killed Plasmodium berghei blood parasite vaccine.

J R Murphy, M J Lefford
PMCID: PMC422231  PMID: 365770

Abstract

Random-bred mice were immunized with a nonliving antigen prepared from mixed-blood forms of Plasmodium berghei, strain NYU-2, in combination with Corynebacterium parvum and/or living BCG. A high proportion of intravenously immunized mice survived virulent challenge, but subcutaneous vaccination was less effective. Vaccinated mice developed a patent infection after challenge similar to that observed in normal controls. However, between days 12 to 20 postchallenge, infections in some vaccinated mice became subpatent, whereas infections in all normal controls progressed until death. The incidence of recrudescent infection was low and, eventually, a state of sterile immunity was established. The capacity of vaccinated mice to withstand P. berghei challenge was sustained at a fairly stable level for the 6-month period of observation. Mice that had survived a primary infection with P. berghei almost completely suppressed a second and larger challenge with the same organism.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brown K. N., Brown I. N., Hills L. A. Immunity to malaria. I. Protection against Plasmodium knowlesi shown by monkeys sensitized with drug-suppressed infections or by dead parasites in Freund's adjuvant. Exp Parasitol. 1970 Oct;28(2):304–317. doi: 10.1016/0014-4894(70)90101-3. [DOI] [PubMed] [Google Scholar]
  2. Cabrera E. J., Barr M. L., Silverman P. H. Long-term studies on rhesus monkeys (Macaca mulatta) immunized against Plasmodium knowlesi. Infect Immun. 1977 Feb;15(2):461–465. doi: 10.1128/iai.15.2.461-465.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Celada F. The cellular basis of immunologic memory. Prog Allergy. 1971;15:223–267. [PubMed] [Google Scholar]
  4. D'Antonio L. E. Plasmodium berghei: vaccination of mice against malaria with heat inactivated parasitized blood. Exp Parasitol. 1972 Feb;31(1):82–87. doi: 10.1016/0014-4894(72)90050-1. [DOI] [PubMed] [Google Scholar]
  5. D'Antonio L. E., Spira D. T., Fu R. C., Dagnillo D. M., Silverman P. H. Malaria resistance: artificial induction with a partially purified plasmodial fraction. Science. 1970 May 29;168(3935):1117–1118. doi: 10.1126/science.168.3935.1117. [DOI] [PubMed] [Google Scholar]
  6. Desowitz R. S. Plasmodium berghei: immunologic enhancement of antigen by adjuvant addition. Exp Parasitol. 1975 Aug;38(1):6–13. doi: 10.1016/0014-4894(75)90032-6. [DOI] [PubMed] [Google Scholar]
  7. Martin W. J., Finerty J., Rosenthal A. Isolation of Plasmodium berghei (malaria) parasites by ammonium chloride lysis of infected erythrocytes. Nat New Biol. 1971 Oct 27;233(43):260–261. doi: 10.1038/newbio233260a0. [DOI] [PubMed] [Google Scholar]
  8. Mitchell G. H., Butcher G. A., Langhorne J., Cohen S. A freeze-dried merozoite vaccine effective against Plasmodium knowlesi malaria. Clin Exp Immunol. 1977 May;28(2):276–279. [PMC free article] [PubMed] [Google Scholar]
  9. North R. J., Deissler J. F. Nature of "memory" in T-cell mediated antibacterial immunity: cellular parameters that distinguish between the active immune response and a state of "memory". Infect Immun. 1975 Oct;12(4):761–767. doi: 10.1128/iai.12.4.761-767.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Playfair J. H., De Souza J. B., Cottrell B. J. Protection of mice against malaria by a killed vaccine: differences in effectiveness against P. yoelii and P. berghei. Immunology. 1977 Oct;33(4):507–515. [PMC free article] [PubMed] [Google Scholar]
  11. Saul K. W., Kreier J. P. Plasmodium berghei: immunization of rats with antigens from a population of free parasites rich in merozoites. Tropenmed Parasitol. 1977 Sep;28(3):302–318. [PubMed] [Google Scholar]
  12. Trizio D., Cudkowicz G. Effect of selective T cell priming on anti-sheep and anti-hapten humoral responses. I. Acceleration, augmentation, and reversal of IgG:IgM ratios. J Immunol. 1978 Mar;120(3):1021–1027. [PubMed] [Google Scholar]
  13. Trizio D., Cudkowicz G. Effect of selective T cell priming on anti-sheep and anti-hapten humoral responses. II. Separation by nylon wool columns of the activated lymphocytes. J Immunol. 1978 Mar;120(3):1028–1032. [PubMed] [Google Scholar]
  14. Wellde B. T., Sadun E. H. Resistance produced in rats and mice by exposure to irradiated Plasmodium berghei. Exp Parasitol. 1967 Dec;21(3):310–324. doi: 10.1016/0014-4894(67)90091-4. [DOI] [PubMed] [Google Scholar]
  15. Wellde B. T., Ward R. A., Ueoka R. Aspects of immunity in mice inoculated with irradiated Plasmodium berghei. Mil Med. 1969 Sep;134(10):1153–1164. [PubMed] [Google Scholar]
  16. Zuckerman A., Hamburger Y., Spira D. Active immunization of rats with a cell-free extract of the erythrocytic parasites of Plasmodium berghei. Exp Parasitol. 1967 Aug;21(1):84–97. doi: 10.1016/0014-4894(67)90071-9. [DOI] [PubMed] [Google Scholar]

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