Abstract
Cells infected with herpesvirus type 1 or type 2 develop surface antigens which can be detected by immunofluorescence. Using the indirect immunofluorescence technique, we determined antibody titers to the surface antigens in 20 sera from persons with known type 1 virus infections, 20 sera from persons with known type 2 virus infections, 30 sera from women with cervical cancer, and 30 sera from matched control women. There was a good correlation between antibody activity to the surface antigens and neutralizing antibody activity for both herpesvirus type 1 and herpesvirus type 2 in all groups of sera examined. Women with cervical cancer did not have unusually high or low titers of antibody activity to the surface antigens. In the selected sera examined, analysis of antibodies to the surface antigens was not superior to the microneutralization test in distinguishing women with cervical cancer from control women.
Full text
PDF





Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cerottini J. C., Brunner K. T. Localization of mouse isoantigens on the cell surface as revealed by immunofluorescence. Immunology. 1967 Oct;13(4):395–403. [PMC free article] [PubMed] [Google Scholar]
- Chen J. H., Purchase H. G. Surface antigen on chick kidney cells infected with the herpesvirus of Marek's disease. Virology. 1970 Feb;40(2):410–412. doi: 10.1016/0042-6822(70)90421-6. [DOI] [PubMed] [Google Scholar]
- Duff R., Rapp F. Reaction of serum from pregnant hamsters with surface of cells transformed by SV40. J Immunol. 1970 Aug;105(2):521–523. [PubMed] [Google Scholar]
- Dunkel V. C., Zeigel R. F. Studies on the relation of membrane immunofluorescence to Epstein-Barr virus infection. J Natl Cancer Inst. 1970 Jan;44(1):133–144. [PubMed] [Google Scholar]
- Figueroa M. E., Rawls W. E. Biological markers for differentiation of herpes-virus strains of oral and genital origin. J Gen Virol. 1969 Mar;4(2):259–267. doi: 10.1099/0022-1317-4-2-259. [DOI] [PubMed] [Google Scholar]
- Geder L., Skinner G. R. Differentiation between type 1 and type 2 strains of herpes simplex virus by an indirect immunofluorescent technique. J Gen Virol. 1971 Aug;12(2):179–182. doi: 10.1099/0022-1317-12-2-179. [DOI] [PubMed] [Google Scholar]
- Hahon N. Cell surface antigen induced by venezuelan equine encephalomyelitis virus. Infect Immun. 1970 Dec;2(6):713–715. doi: 10.21236/ad0872045. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hampar B., Miyamoto K., Martos L. M. Serologic classification of Herpes simplex viruses. J Immunol. 1971 Feb;106(2):580–582. [PubMed] [Google Scholar]
- Ishimoto A., Ito Y. Specific surface antigen in Shope papilloma cells. Virology. 1969 Nov;39(3):595–597. doi: 10.1016/0042-6822(69)90109-3. [DOI] [PubMed] [Google Scholar]
- Klein G. Immunological studies on Burkitt's lymphoma. Postgrad Med J. 1971 Mar;47(545):141–155. doi: 10.1136/pgmj.47.545.141. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Klein G., Pearson G., Nadkarni J. S., Nadkarni J. J., Klein E., Henle G., Henle W., Clifford P. Relation between Epstein-Barr viral and cell membrane immunofluorescence of Burkitt tumor cells. I. Dependence of cell membrane immunofluorescence on presence of EB virus. J Exp Med. 1968 Nov 1;128(5):1011–1020. doi: 10.1084/jem.128.5.1011. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kluchareva T. E., Shachanina K. L., Belova S., Chibisova V., Deichman G. I. Use of immunofluorescence for detection of specific membrane antigen in simian virus 40-infected nontransformed cells. J Natl Cancer Inst. 1967 Nov;39(5):825–832. [PubMed] [Google Scholar]
- Lowry S. P., Bronson D. L., Rawls W. E. Characterization of the abortive infection of chick embryo cells by herpesvirus type I. J Gen Virol. 1971 Apr;11(1):47–51. doi: 10.1099/0022-1317-11-1-47. [DOI] [PubMed] [Google Scholar]
- Lowry S. P., Melnick J. L., Rawls W. E. Investigation of plaque formation in chick embryo cells as a biological marker for distinguishing herpes virus type 2 from type 1. J Gen Virol. 1971 Jan;10(1):1–9. doi: 10.1099/0022-1317-10-1-1. [DOI] [PubMed] [Google Scholar]
- Nahmias A. J., Josey W. E., Naib Z. M., Luce C. F., Guest B. A. Antibodies to Herpesvirus hominis types 1 and 2 in humans. II. Women with cervical cancer. Am J Epidemiol. 1970 Jun;91(6):547–552. doi: 10.1093/oxfordjournals.aje.a121166. [DOI] [PubMed] [Google Scholar]
- Naib Z. M., Nahmias A. J., Josey W. E., Kramer J. H. Genital herpetic infection. Association with cervical dysplasia and carcinoma. Cancer. 1969 Apr;23(4):940–945. doi: 10.1002/1097-0142(196904)23:4<940::aid-cncr2820230432>3.0.co;2-e. [DOI] [PubMed] [Google Scholar]
- RODRIGUEZ J., DEINHARDT F. Preparation of a semipermanent mounting medium for fluorescent antibody studies. Virology. 1960 Oct;12:316–317. doi: 10.1016/0042-6822(60)90205-1. [DOI] [PubMed] [Google Scholar]
- Rawls W. E., Iwamoto K., Adam E., Melnick J. L., Green G. H. Herpesvirus-type-2 antibodies and carcinoma of the cervix. Lancet. 1970 Nov 28;2(7683):1142–1143. doi: 10.1016/s0140-6736(70)92349-4. [DOI] [PubMed] [Google Scholar]
- Rawls W. E., Iwamoto K., Adam E., Melnick J. L. Measurement of antibodies to herpesvirus types 1 and 2 in human sera. J Immunol. 1970 Mar;104(3):599–606. [PubMed] [Google Scholar]
- Rawls W. E., Kaufman R. H. Herpesvirus and other factors related to the genesis of cervical cancer. Clin Obstet Gynecol. 1970 Dec;13(4):857–872. doi: 10.1097/00003081-197012000-00004. [DOI] [PubMed] [Google Scholar]
- Roizman B., Spear P. G. Herpesvirus antigens on cell membranes detected by centrifugation of membrane-antibody complexes. Science. 1971 Jan 22;171(3968):298–300. doi: 10.1126/science.171.3968.298. [DOI] [PubMed] [Google Scholar]
- Royston I., Aurelian L. Immunofluorescent detection of herpesvirus antigens in exfoliated cells from human cervical carcinoma. Proc Natl Acad Sci U S A. 1970 Sep;67(1):204–212. doi: 10.1073/pnas.67.1.204. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Royston I., Aurelian L. The association of genital herpesvirus with cervical atypia and carcinoma in situ. Am J Epidemiol. 1970 Jun;91(6):531–538. doi: 10.1093/oxfordjournals.aje.a121164. [DOI] [PubMed] [Google Scholar]
- TEVETHIA S. S., KATZ M., RAPP F. NEW SURFACE ANTIGEN IN CELLS TRANSFORMED BY SIMIAN PAPOVAVIRUS SV40. Proc Soc Exp Biol Med. 1965 Jul;119:896–901. doi: 10.3181/00379727-119-30330. [DOI] [PubMed] [Google Scholar]
- Ueda Y., Ito M., Tagaya I. A specific surface antigen induced by poxvirus. Virology. 1969 May;38(1):180–182. doi: 10.1016/0042-6822(69)90141-x. [DOI] [PubMed] [Google Scholar]