Abstract
A soluble viral antigen with possible diagnostic utility was obtained from blue-tongue virus-infected lamb kidney cells. It is a noninfectious proteinaceous substance without nucleic acid. The antigen is immunologically identical to one component of the viral particle, and in agar-gel diffusion tests it reacted with antiserum against several blue-tongue virus strains.
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- ALLISON A. C., HUMPHREY J. H. A theoretical and experimental analysis of double diffusion precipitin reactions in gels, and its application to characterization of antigens. Immunology. 1960 Jan;3:95–106. [PMC free article] [PubMed] [Google Scholar]
- ALLISON A. C., HUMPHREY J. H. Estimation of the size of antigens by gel diffusion methods. Nature. 1959 Jun 6;183(4675):1590–1592. doi: 10.1038/1831590a0. [DOI] [PubMed] [Google Scholar]
- Avrameas S., Ternynck T. Biologically active water-insoluble protein polymers. I. Their use for isolation of antigens and antibodies. J Biol Chem. 1967 Apr 10;242(7):1651–1659. [PubMed] [Google Scholar]
- Avrameas S., Ternynck T. The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents. Immunochemistry. 1969 Jan;6(1):53–66. doi: 10.1016/0019-2791(69)90178-5. [DOI] [PubMed] [Google Scholar]
- BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Campbell D. H., Luescher E., Lerman L. S. Immunologic Adsorbents: I. Isolation of Antibody by Means of a Cellulose-Protein Antigen. Proc Natl Acad Sci U S A. 1951 Sep;37(9):575–578. doi: 10.1073/pnas.37.9.575. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DISCHE Z. Qualitative and quantitative colorimetric determination of heptoses. J Biol Chem. 1953 Oct;204(2):983–997. [PubMed] [Google Scholar]
- Jochim M. M., Chow T. L. Immunodiffusion of bluetongue virus. Am J Vet Res. 1969 Jan;30(1):33–41. [PubMed] [Google Scholar]
- KLONTZ G. W., SVEHAG S. E., GORHAM J. R. A study by the agar diffusion technique of precipitating antibody directed against blue tongue virus and its relation to homotypic neutralizing antibody. Arch Gesamte Virusforsch. 1962;12:259–268. doi: 10.1007/BF01258136. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Lindsey N. J., Chow T. L. Preservation of primary bovine embryonic kidney cells with dimethyl sulfoxide. Appl Microbiol. 1969 Mar;17(3):484–485. doi: 10.1128/am.17.3.484-485.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Luedke A. J., Jochim M. M. Clinical and serologic responses in vaccinated sheep given challenge inoculation with isolates of bluetongue virus. Am J Vet Res. 1968 Apr;29(4):841–851. [PubMed] [Google Scholar]
- MCKERCHER D. G., MCGOWAN B., Jr, CABASSO V. J., ROBERTS G. I., SAITO J. K. Studies on bluetongue. III. The development of a modified live virus vaccine employing American strains of bluetongue virus. Am J Vet Res. 1957 Apr;18(67):310–316. [PubMed] [Google Scholar]
- Marusyk R. G., Norrby E., Lundqvist U. Biophysical comparison of two canine adenoviruses. J Virol. 1970 Apr;5(4):507–512. doi: 10.1128/jvi.5.4.507-512.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Metcalf H. E., Jochim M. M. Bluetongue in cattle: efficacy of the agar gel precipitin test. Am J Vet Res. 1970 Oct;31(10):1743–1749. [PubMed] [Google Scholar]
- Porter R. R. Separation of proteins and protein sub-units. Br Med Bull. 1966 May;22(2):164–167. doi: 10.1093/oxfordjournals.bmb.a070460. [DOI] [PubMed] [Google Scholar]
- SQUIRE P. G. A RELATIONSHIP BETWEEN THE MOLECULAR WEIGHTS OF MACROMOLECULES AND THEIR ELUTION VOLUMES BASED ON A MODEL FOR SEPHADEX GEL FILTRATION. Arch Biochem Biophys. 1964 Sep;107:471–478. doi: 10.1016/0003-9861(64)90303-0. [DOI] [PubMed] [Google Scholar]
- Silman I., Katchalski E. Water-insoluble derivatives of enzymes, antigens, and antibodies. Annu Rev Biochem. 1966;35:873–908. doi: 10.1146/annurev.bi.35.070166.004301. [DOI] [PubMed] [Google Scholar]
- Trainer D. O., Jochim M. M. Serologic evidence of bluetongue in wild ruminants of North America. Am J Vet Res. 1969 Nov;30(11):2007–2011. [PubMed] [Google Scholar]
- Verwoerd D. W. Purification and characterization of bluetongue virus. Virology. 1969 Jun;38(2):203–212. doi: 10.1016/0042-6822(69)90361-4. [DOI] [PubMed] [Google Scholar]
- ZWARTOUW H. T., WESTWOOD J. C., HARRIS W. J. ANTIGENS FROM VACCINIA VIRUS PARTICLES. J Gen Microbiol. 1965 Jan;38:39–45. doi: 10.1099/00221287-38-1-39. [DOI] [PubMed] [Google Scholar]




