Abstract
Rabbits sensitized intravenously with heat-killed Mycobacterium tuberculosis (strain H37Ra) suspended in mineral oil developed a strong pulmonary granulomatous response which reached its peak about 3 to 4 weeks after injection. Alveolar cells (4 × 106 cells/ml of tissue culture medium 199) procured 6 weeks after sensitization showed extensive development of multinucleated giant cells after 12 hr of incubation in tissue culture flasks containing heat-killed H37Ra (5 μg/ml). Giant cells measured 80 μm to 2.5 mm in length and contained between 30 and 700 nuclei. In contrast, no giant cells were observed when similar samples of the same cell populations were incubated in flasks containing: (i) no mycobacteria; (ii) heat-killed Escherichia coli; (iii) heat-killed Bacillus subtilis; (iv) latex particles; (v) ovalbumin; or (vi) phytohemagglutinin. The addition of immune (anti-H37Ra) sera potentiated the phenomenon of giant cell formation. In addition, supernatant fluids obtained from sensitive alveolar cells incubated with H37Ra were capable of inducing giant cell formation when incubated with nonsensitized alveolar cells. The results suggest that fusion of alveolar macrophages is mediated by an immunological mechanism.
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- ALLEN J. M., BRIEGER E. M., REES R. J. ELECTRON MICROSCOPY OF THE HOST-CELL PARASITE RELATION IN MURINE LEPROSY. J Pathol Bacteriol. 1965 Jan;89:301–306. doi: 10.1002/path.1700890131. [DOI] [PubMed] [Google Scholar]
- CHANOCK R. M. Association of a new type of cytopathogenic myxovirus with infantile croup. J Exp Med. 1956 Oct 1;104(4):555–576. doi: 10.1084/jem.104.4.555. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Comoglio P. M., Ottino G., Cantino D. Experimental study on development and behaviour of the multinucleated giant cells "in vitro". J Reticuloendothel Soc. 1971 May;9(5):397–408. [PubMed] [Google Scholar]
- David J. R. Delayed hypersensitivity in vitro: its mediation by cell-free substances formed by lymphoid cell-antigen interaction. Proc Natl Acad Sci U S A. 1966 Jul;56(1):72–77. doi: 10.1073/pnas.56.1.72. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Galindo B., Myrvik Q. N. Migratory response of granulomatous alveolar cells from BCG-sensitized rabbits. J Immunol. 1970 Jul;105(1):227–237. [PubMed] [Google Scholar]
- MARSTON R. Q. Cytopathogenic effects of hemadsorption virus type I. Proc Soc Exp Biol Med. 1958 Aug-Sep;98(4):853–856. doi: 10.3181/00379727-98-24207. [DOI] [PubMed] [Google Scholar]
- MYRVIK Q., LEAKE E. S., FARISS B. Studies on pulmonary alveolar macrophages from the normal rabbit: a technique to procure them in a high state of purity. J Immunol. 1961 Feb;86:128–132. [PubMed] [Google Scholar]
- Matthews J. L., Martin J. H., Race G. J., Collins E. J. Giant-cell centrioles. Science. 1967 Mar 17;155(3768):1423–1424. doi: 10.1126/science.155.3768.1423. [DOI] [PubMed] [Google Scholar]
- McLIMANS W. F., DAVIS E. V., GLOVER F. L., RAKE G. W. The submerged culture of mammalian cells; the spinner culture. J Immunol. 1957 Nov;79(5):428–433. [PubMed] [Google Scholar]
- Sutton J. S., Weiss L. Transformation of monocytes in tissue culture into macrophages, epithelioid cells, and multinucleated giant cells. An electron microscope study. J Cell Biol. 1966 Feb;28(2):303–332. doi: 10.1083/jcb.28.2.303. [DOI] [PMC free article] [PubMed] [Google Scholar]