Figure 1.

Deletion of eight thiol peroxidases causes growth defects and reduces life span in yeast. (A) Decreased colony growth of ∆8 cells. Overnight cultures of WT, ∆3, ∆5, and two independent ∆8 isolates were diluted to an OD₆₀₀ of 0.3. Then, 5 µl of 10-fold dilution of each cell suspension was spotted onto YPD plates and photographed 3 days later. (B) Reduced cell growth of ∆8 cells. Growth of WT and ∆8 cells in liquid YPD medium was monitored by measuring the OD₆₀₀. (C) Increased division time of ∆8 cells. Division time (in minutes) was calculated for each strain and plotted. (D) Replicative life span of WT and ∆8 cells. Numbers in brackets next to each strain indicate mean life span. The y-axis represents the fraction of survivors (fraction of mother cells capable of budding) at each generation (x-axis).