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. 1972 May;5(5):814–825. doi: 10.1128/iai.5.5.814-825.1972

Activity of Macrophage and Neutrophil Cellular Fractions from Normal and Immune Sheep Against Listeria monocytogenes

P M Outteridge a,1, J W Osebold a, Y C Zee a
PMCID: PMC422445  PMID: 4629255

Abstract

Cellular immunity to Listeria monocytogenes infection was studied by assaying for antibacterial activity in fractions of leukocytes collected from the peritoneal cavity, lungs, and mammary glands of immunized sheep. The cells were collected in populations that were largely either macrophages or neutrophils. Mechanically disrupted cells were divided into nuclear, lysosomal, and supernatant fluid fractions and then subjected to freezing and thawing. Comparison with similarly treated rabbit cells showed that greater fragility exists in the lysosomes of sheep cells, as indicated by the amount of acid phosphatase activity released. Inhibition of bacterial growth was assayed in a broth medium at pH 4.6. As expected, nuclear and lysosomal fractions from neutrophils were inhibitory. Some antibacterial activity was found in nuclear fractions of macrophages. The lysosomes of macrophages collected from the peritoneal cavity and the mammary gland did not inhibit the growth of L. monocytogenes. Peritoneal macrophages were allowed to interact with sensitized lymphocytes and an avirulent strain of L. monocytogenes for 4 hr prior to disruption and fractionation, but antibacterial activity was not detected. Pulmonary alveolar macrophages from 5 out of 16 sheep contained Listeria inhibitory activity in their lysosomes. The mechanism was inhibitory but not bactericidal.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. COHN Z. A., BENSON B. THE DIFFERENTIATION OF MONONUCLEAR PHAGOCYTES. MORPHOLOGY, CYTOCHEMISTRY, AND BIOCHEMISTRY. J Exp Med. 1965 Jan 1;121:153–170. doi: 10.1084/jem.121.1.153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. COHN Z. A., HIRSCH J. G. The influence of phagocytosis on the intracellular distribution of granule-associated components of polymorphonuclear leucocytes. J Exp Med. 1960 Dec 1;112:1015–1022. doi: 10.1084/jem.112.6.1015. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. COHN Z. A. The fate of bacteria within phagocytic cells. I. The degradation of isotopically labeled bacteria by polymorphonuclear leucocytes and macrophages. J Exp Med. 1963 Jan 1;117:27–42. doi: 10.1084/jem.117.1.27. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Coppel S., Youmans G. P. Specificity of the anamnestic response produced by Listeria monocytogenes or Mycobacterium tuberculosis to challenge with Listeria monocytogenes. J Bacteriol. 1969 Jan;97(1):127–133. doi: 10.1128/jb.97.1.127-133.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dannenberg A. M., Jr Cellular hypersensitivity and cellular immunity in the pathogensis of tuberculosis: specificity, systemic and local nature, and associated macrophage enzymes. Bacteriol Rev. 1968 Jun;32(2):85–102. doi: 10.1128/br.32.2.85-102.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. ELBERG S. S., SCHNEIDER P., FONG J. Cross-immunity between Brucella melitensis and Mycobacterium tuberculosis; intracellular behavior of Brucella melitensis in monocytes from vaccinated animals. J Exp Med. 1957 Oct 1;106(4):545–554. doi: 10.1084/jem.106.4.545. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Godal T., Rees R. J., Lamvik J. O. Lymphocyte-mediated modification of blood-derived macrophage function in vitro; inhibition of growth of intracellular mycobacteria with lymphokines. Clin Exp Immunol. 1971 Apr;8(4):625–637. [PMC free article] [PubMed] [Google Scholar]
  8. HEISE E. R., MYRVIK Q. N., LEAKE E. S. EFFECT OF BACILLUS CALMETTE-GU'ERIN ON THE LEVELS OF ACID PHOSPHATASE, LYSOZYME AND CATHEPSIN IN RABBIT ALVEOLAR MACROPHAGES. J Immunol. 1965 Jul;95:125–130. [PubMed] [Google Scholar]
  9. Holub M., Hauser R. E. Lung alveolar histiocytes engaged in antibody production. Immunology. 1969 Aug;17(2):207–226. [PMC free article] [PubMed] [Google Scholar]
  10. Janoff A., Bean M. A., Schuller E. Mediators of inflammation in leukocyte lysosomes. 3. Studies on lysosomes of rabbit peritoneal macrophages. Life Sci. 1965 Dec;4(24):2361–2372. doi: 10.1016/0024-3205(65)90291-2. [DOI] [PubMed] [Google Scholar]
  11. Kurashige S., Osawa N., Kawakami M., Mitsuhashi S. Experimental salmonellosis. X. Cellular immunity and its antibody in mouse mononuclear phagocytes. J Bacteriol. 1967 Oct;94(4):902–906. doi: 10.1128/jb.94.4.902-906.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LEAKE E. S., MYRVIK Q. N. DIFFERENTIAL RELEASE OF LYSOZYME AND ACID PHOSPHATASE FROM SUB-CELLULAR GRANULES OF NORMAL RABBIT ALVEOLAR MACROPHAGES. Br J Exp Pathol. 1964 Aug;45:384–392. [PMC free article] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. Leake E. S., Evans D. G., Myrvik Q. N. Ultrastructural patterns of bacterial breakdown in normal and granulomatous rabbit alveolar macrophages. J Reticuloendothel Soc. 1971 Feb;9(2):174–199. [PubMed] [Google Scholar]
  15. MACKANESS G. B. Cellular resistance to infection. J Exp Med. 1962 Sep 1;116:381–406. doi: 10.1084/jem.116.3.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. MUSCHEL L. H., TREFFERS H. P. Quantitative studies on the bactericidal actions of serum and complement. I. A rapid photometric growth assay for bactericidal activity. J Immunol. 1956 Jan;76(1):1–10. [PubMed] [Google Scholar]
  17. Mackaness G. B. The immunology of antituberculous immunity. Am Rev Respir Dis. 1968 Mar;97(3):337–344. doi: 10.1164/arrd.1968.97.3.337. [DOI] [PubMed] [Google Scholar]
  18. NJOKU-OBI A. N., OSEBOLD J. W. Studies on mechanisms of immunity in listeriosis. I. Interaction of peritoneal exudate cells from sheep with Listeria monocytogenes in vitro. J Immunol. 1962 Aug;89:187–194. [PubMed] [Google Scholar]
  19. NORTH R. J., MACKANESS G. B. ELECTRON MICROSCOPICAL OBSERVATIONS ON THE PERITONEAL MACROPHAGES OF NORMAL MICE AND MICE IMMUNISED WITH LISTERIA MONOCYTOGENES. I. STRUCTURE OF NORMAL MACROPHAGES AND THE EARLY CYTOPLASMIC RESPONSE TO THE PRESENCE OF INGESTED BACTERIA. Br J Exp Pathol. 1963 Dec;44:601–607. [PMC free article] [PubMed] [Google Scholar]
  20. North R. J. The localization by electron microscopy of acid phosphatase activity in guinea pig macrophages. J Ultrastruct Res. 1966 Sep;16(1):96–108. doi: 10.1016/s0022-5320(66)80025-4. [DOI] [PubMed] [Google Scholar]
  21. OUTTERIDGE P. M., ROCK J. D., LASCELLES A. K. THE IMMUNE RESPONSE OF THE MAMMARY GLAND AND REGIONAL LYMPH NODE FOLLOWING ANTIGENIC STIMULATION. Aust J Exp Biol Med Sci. 1965 Jun;43:265–274. doi: 10.1038/icb.1965.25. [DOI] [PubMed] [Google Scholar]
  22. Osebold J. W., Aalund O. Interpretation of serum agglutinating antibodies to Listeria monocytogenes by immunoglobulin differentiation. J Infect Dis. 1968 Apr;118(2):139–148. doi: 10.1093/infdis/118.2.139. [DOI] [PubMed] [Google Scholar]
  23. Osebold J. W., Outteridge P. M., Pearson L. D. Mutation of Listeria monocytogenes After Prolonged In Vivo Survival in Diffusion Chambers. Infect Immun. 1970 Feb;1(2):209–211. doi: 10.1128/iai.1.2.209-211.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Outteridge P. M., Osebold J. W., Zee Y. C. The mammary gland of the ewe as a source of neutrophils and macrophages for repeated collections. J Reticuloendothel Soc. 1971 Nov;10(5):440–448. [PubMed] [Google Scholar]
  25. Patterson R. J., Youmans G. P. Demonstration in tissue culture of lymphocyte-mediated immunity to tuberculosis. Infect Immun. 1970 Jun;1(6):600–603. doi: 10.1128/iai.1.6.600-603.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Paul B. B., Strauss R. R., Jacobs A. A., Sbarra A. J. Function of h(2)o(2), myeloperoxidase, and hexose monophosphate shunt enzymes in phagocytizing cells from different species. Infect Immun. 1970 Apr;1(4):338–344. doi: 10.1128/iai.1.4.338-344.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. ROBBINS E., GONATAS N. K. IN VITRO SELECTION OF THE MITOTIC CELL FOR SUBSEQUENT ELECTRON MICROSCOPY. J Cell Biol. 1964 Feb;20:356–359. doi: 10.1083/jcb.20.2.356. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Rowley D. Phagocytosis and immunity. The carrier state and cellular immunity. Experientia. 1966 Jan 15;22(1):9–13. [PubMed] [Google Scholar]
  29. SAITO K., AKIYAMA T., NAKANO M., USHBA D. Interaction between Salmonella enteritidis and tissue cultured macrophages derived from immunized animals. Jpn J Microbiol. 1960 Oct;4:395–407. doi: 10.1111/j.1348-0421.1960.tb00188.x. [DOI] [PubMed] [Google Scholar]
  30. WIENER E., SHILO M., BECK A. EFFECT OF BACTERIAL LIPOPOLYSACCHARIDES ON MOUSE PERITONEAL LEUKOCYTES. Lab Invest. 1965 May;14:475–487. [PubMed] [Google Scholar]
  31. Weiser R. S. Antitissue versus antimicrobial cellular immunity: a perspective. J Reticuloendothel Soc. 1971 Jul;10(1):17–27. [PubMed] [Google Scholar]
  32. YOUMANS A. S., YOUMANS G. P. Effect of mycosuppressin on the respiration and growth of Mycobacterium tuberculosis. J Bacteriol. 1962 Oct;84:708–715. doi: 10.1128/jb.84.4.708-715.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Youmans G. P. The role of lymphocytes and other factors in antimicrobial cellular immunity. J Reticuloendothel Soc. 1971 Jul;10(1):100–119. [PubMed] [Google Scholar]
  34. ZUCKER-FRANKLIN D., HIRSCH J. G. ELECTRON MICROSCOPE STUDIES ON THE DEGRANULATION OF RABBIT PERITONEAL LEUKOCYTES DURING PHAGOCYTOSIS. J Exp Med. 1964 Oct 1;120:569–576. doi: 10.1084/jem.120.4.569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Zeya H. I., Spitznagel J. K. Cationic protein-bearing granules of polymorphonuclear leukocytes: separation from enzyme-rich granules. Science. 1969 Mar 7;163(3871):1069–1071. doi: 10.1126/science.163.3871.1069. [DOI] [PubMed] [Google Scholar]
  36. Zeya H. I., Spitznagel J. K. Cationic proteins of polymorphonuclear leukocyte lysosomes. I. Resolution of antibacterial and enzymatic activities. J Bacteriol. 1966 Feb;91(2):750–754. doi: 10.1128/jb.91.2.750-754.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]

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