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. 1972 Jun;5(6):890–895. doi: 10.1128/iai.5.6.890-895.1972

Study of the One-Step Growth Curve of Equine Infectious Anemia Virus by Immunofluorescence

Chuzo Ushimi a,1, James B Henson a, John R Gorham a
PMCID: PMC422458  PMID: 4344094

Abstract

Primary horse leukocyte cultures were inoculated with 2 or 10 50% tissue culture infective doses (TCID50) of equine infectious anemia (EIA) virus per cell, and the titer of cell-associated and fluid-phase virus was determined from 1 to 72 hr postinoculation (PI). Cover slips were collected from 4 to 72 hr PI and stained for EIA viral antigen by the indirect immunofluorescent (FA) technique. Viral replication was detected after a latent period of approximately 18 to 24 hr and reached peak titers of approximately 104.5 to 106 TCID50/0.5 ml from 48 to 72 hr PI. The fluid phase contained 101 to 102 TCID50/0.5 ml more virus than the cells. Viral antigen was first detected by FA from 18 to 24 hr PI. Approximately 75% of the cells contained antigen in their cytoplasm 72 hr PI. The FA technique is a sensitive method for detecting EIA virus in horse leukocyte cultures.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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