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. 1972 Jun;5(6):968–975. doi: 10.1128/iai.5.6.968-975.1972

Isolation of the Outer Sheath of Leptospira and Its Immunogenic Properties in Hamsters

N E Auran 1, R C Johnson 1, D M Ritzi 1
PMCID: PMC422473  PMID: 4635506

Abstract

Spherical forms of Leptospira interrogans serotype canicola Hond Utrecht IV were induced with 1 m NaCl. Electron micrographs of these salt-altered cells (SAC) revealed that the outer envelope or sheath had pulled away from the protoplasmic cylinder. Treatment of SAC with 0.02% sodium lauryl sulfate solubilized the sheath and released the protoplasmic cylinder. Further processing of the solubilized sheath yielded a pellet which displayed a membrane structure in electron micrographs. The released protoplasmic cylinder showed loss of intracellular organization and the outer envelope present in normal cells. Immunization of hamsters with whole formalized cells, SAC, or sheath in doses as low as 10 μg/animal protected them from death upon challenge with virulent canicola 27.

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These references are in PubMed. This may not be the complete list of references from this article.

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