Abstract
Lactoferrin is contained in cytoplasmic granules of human polymorphonuclear leukocytes. Upon centrifugation, it sediments in a band of granules that also contain 50% of the lysozyme activity. This granule class is distinct from others associated with alkaline phosphatase and peroxidase. The granules are latent for lactoferrin as only lysed granules have the capacity specifically to inhibit antigen binding by anti-lactoferrin serum.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baggiolini M., De Duve C., Masson P. L., Heremans J. F. Association of lactoferrin with specific granules in rabbit heterophil leukocytes. J Exp Med. 1970 Mar 1;131(3):559–570. doi: 10.1084/jem.131.3.559. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Baggiolini M., Hirsch J. G., De Duve C. Resolution of granules from rabbit heterophil leukocytes into distinct populations by zonal sedimentation. J Cell Biol. 1969 Feb;40(2):529–541. doi: 10.1083/jcb.40.2.529. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bullen J. J., Rogers H. J., Lewin J. E. The bacteriostatic effect of serum on Pasteurella septica and its abolition by iron compounds. Immunology. 1971 Mar;20(3):391–406. [PMC free article] [PubMed] [Google Scholar]
- FARR R. S. A quantitative immunochemical measure of the primary interaction between I BSA and antibody. J Infect Dis. 1958 Nov-Dec;103(3):239–262. doi: 10.1093/infdis/103.3.239. [DOI] [PubMed] [Google Scholar]
- Green I., Kirkpatrick C. H., Dale D. C. Lactoferrin--specific localization in the nuclei of human polymorphonuclear neutrophilic leukocytes. Proc Soc Exp Biol Med. 1971 Sep;137(4):1311–1317. doi: 10.3181/00379727-137-35779. [DOI] [PubMed] [Google Scholar]
- JANOFF A., ZWEIFACH B. W. Inactivation of bacterial exotoxins and endotoxin by iron. In vitro studies. J Exp Med. 1960 Jul 1;112:23–34. doi: 10.1084/jem.112.1.23. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
- Masson P. L., Heremans J. F. Metal-combining properties of human lactoferrin (red milk protein). 1. The involvement of bicarbonate in the reaction. Eur J Biochem. 1968 Dec 5;6(4):579–584. doi: 10.1111/j.1432-1033.1968.tb00484.x. [DOI] [PubMed] [Google Scholar]
- Masson P. L., Heremans J. F., Schonne E. Lactoferrin, an iron-binding protein in neutrophilic leukocytes. J Exp Med. 1969 Sep 1;130(3):643–658. doi: 10.1084/jem.130.3.643. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mogensen C. E. The glomerular permeability determined by dextran clearance using Sephadex gel filtration. Scand J Clin Lab Invest. 1968;21(1):77–82. doi: 10.3109/00365516809076979. [DOI] [PubMed] [Google Scholar]
- Oram J. D., Reiter B. Inhibition of bacteria by lactoferrin and other iron-chelating agents. Biochim Biophys Acta. 1968 Dec 23;170(2):351–365. doi: 10.1016/0304-4165(68)90015-9. [DOI] [PubMed] [Google Scholar]
- Rogers H. J., Bullen J. J., Cushnie G. H. Iron compounds and resistance to infection. Further experiments with Clostridium welchii type A in vivo and in vitro. Immunology. 1970 Oct;19(4):521–538. [PMC free article] [PubMed] [Google Scholar]
- Welsh I. R., Spitznagel J. K. Distribution of lysosomal enzymes, cationic proteins, and bactericidal substances in subcellular fractions of human polymorphonuclear leukocytes. Infect Immun. 1971 Aug;4(2):97–102. doi: 10.1128/iai.4.2.97-102.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]