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. 1972 Nov;6(5):810–818. doi: 10.1128/iai.6.5.810-818.1972

Immunological and Chemical Studies of the Streptococcal Group O Protein Type Antigens

H Mukasa 1, H D Slade 1
PMCID: PMC422615  PMID: 4118018

Abstract

Two antigens present in group O streptococci have been described and are designated as type antigens. No other antigens are present in extracts of group O cells in significant quantity. Antigen I is a cell wall protein, and II is a nucleoprotein of undetermined cell location. The immunological specificity of the latter resides only in the protein. Both antigens have been extracted from the cell with water or 0.2 n HCl (pH 2) at 100 C, and antigen I can be removed by trypsin. After purification, pH 2 was found to destroy the serological activity of each antigen. Antgens I and II and also the O polysaccharide antigen can be distinguished on a single agar-gel diffusion plate with group O whole-cell antiserum. N- and C-terminal amino acid analyses and total amino acid composition prove that I and II are separate molecular species. Each possesses properties which separate them from the streptococcal M, R, and T proteins. Antigen I separated into three fractions, each possessing serological specificity, on a diethylaminoethyl-Sephadex column at pH 8.6 during an increase in buffer molarity. The protein is considered to possess a multiple unit structure. The results indicate that the O streptococci are limited to two serological types. Group O whole-cell antisera, after adsorption with a strain not containing the O polysaccharide, can now be prepared for the positive identification of the types of group O streptococci.

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Selected References

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  1. Andrews P. Estimation of the molecular weights of proteins by Sephadex gel-filtration. Biochem J. 1964 May;91(2):222–233. doi: 10.1042/bj0910222. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DISCHE Z. Spectrophotometric method for the determination of free pentose and pentose in nucleotides. J Biol Chem. 1949 Nov;181(1):379–392. [PubMed] [Google Scholar]
  3. Fox E. N., Wittner M. K. New observations on the structure and antigenicity of the M proteins of the group A streptococcus. Immunochemistry. 1969 Jan;6(1):11–24. doi: 10.1016/0019-2791(69)90174-8. [DOI] [PubMed] [Google Scholar]
  4. Fox E. N., Wittner M. K. The multiple molecular structure of the M proteins of group A streptococci. Proc Natl Acad Sci U S A. 1965 Oct;54(4):1118–1125. doi: 10.1073/pnas.54.4.1118. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gehrke C. W., Shahrokhi F. Chromatographic separation of n-butyl N-trifluoroacetyl esters of amino acids. Anal Biochem. 1966 Apr;15(1):97–108. doi: 10.1016/0003-2697(66)90252-1. [DOI] [PubMed] [Google Scholar]
  6. HESS E. L., SLADE H. D. An electrophoretic examination of cell-free extracts from various serological types of group A hemolytic streptococci. Biochim Biophys Acta. 1955 Mar;16(3):346–353. doi: 10.1016/0006-3002(55)90237-7. [DOI] [PubMed] [Google Scholar]
  7. JABLON J. M., BRUST B., SASLAW M. S. BETA-HEMOLYTIC STREPTOCOCCI WITH GROUP A AND TYPE II CARBOHYDRATE ANTIGENS. J Bacteriol. 1965 Feb;89:529–534. doi: 10.1128/jb.89.2.529-534.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LANCEFIELD R. C., PERLMANN G. E. Preparation and properties of a protein (R antigen) occurring in streptococci of group A, type 28 and in certain streptococci of other serological groups. J Exp Med. 1952 Jul;96(1):83–97. doi: 10.1084/jem.96.1.83. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LEVY A. L. A paper chromatographic method for the quantitative estimation of amino-acids. Nature. 1954 Jul 17;174(4420):126–127. doi: 10.1038/174126a0. [DOI] [PubMed] [Google Scholar]
  10. LEVY A. L., LI C. H. Corticotropins (ACTH). III. Kinetic studies on the reaction of N-terminal group reagents with alpha-corticotropin, and N-terminal group analysis. J Biol Chem. 1955 Apr;213(2):487–497. [PubMed] [Google Scholar]
  11. MAXTED W. R. The M and T antigens of Streptococcus pyogenes type 2. J Pathol Bacteriol. 1953 Apr;65(2):345–354. doi: 10.1002/path.1700650207. [DOI] [PubMed] [Google Scholar]
  12. Matsuno T., Slade H. D. Composition and properties of a group A streptococcal teichoic acid. J Bacteriol. 1970 Jun;102(3):747–752. doi: 10.1128/jb.102.3.747-752.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Matsuno T., Slade H. D. Group a streptococcal polysaccharide antigens. Infect Immun. 1971 Mar;3(3):385–389. doi: 10.1128/iai.3.3.385-389.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Maxted W. R., Potter E. V. The presence of type 12 M-protein antigen in group G streptococci. J Gen Microbiol. 1967 Oct;49(1):119–125. doi: 10.1099/00221287-49-1-119. [DOI] [PubMed] [Google Scholar]
  15. Mukasa H., Slade H. D. Chemical composition and immunological specificity of the streptococcal group O cell wall polysaccharide antigen. Infect Immun. 1972 May;5(5):707–714. doi: 10.1128/iai.5.5.707-714.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. OTTENS H., WINKLER K. C. Indifferent and haemolytic streptococci possessing group-antigen F. J Gen Microbiol. 1962 Apr;28:181–191. doi: 10.1099/00221287-28-1-181. [DOI] [PubMed] [Google Scholar]
  17. PAKULA R. Extraction of the T antigen of Streptococcus pyogenes. J Gen Microbiol. 1951 Oct;5(4):640–647. doi: 10.1099/00221287-5-4-640. [DOI] [PubMed] [Google Scholar]
  18. PATTISON I. H., MATTHEWS P. R., HOWELL D. G. The type classification of group-B streptococci, with special reference to bovine strains apparently lacking in type polysaccharide. J Pathol Bacteriol. 1955 Jan-Apr;69(1-2):51–60. [PubMed] [Google Scholar]
  19. SLADE H. D., SLAMP W. C. Cell-wall composition and the grouping antigens of Streptococci. J Bacteriol. 1962 Aug;84:345–351. doi: 10.1128/jb.84.2.345-351.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. SLADE H. D., SLAMP W. C. Studies on Streptococcus pyogenes. V. Biochemical and microscopic aspects of cell lysis and digestion by enzymes from Streptomyces albus. J Bacteriol. 1960 Jan;79:103–112. doi: 10.1128/jb.79.1.103-112.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. SMITH J. D., MARKHAM R. Chromatographic studies on nucleic acids; the quantitative analysis of ribonucleic acids. Biochem J. 1950 May;46(5):509–513. doi: 10.1042/bj0460509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Slade H. D., Slamp W. C. Peptidoglycan composition and taxonomy of group D, E, and H streptococci, and Streptococcus mutans. J Bacteriol. 1972 Feb;109(2):691–695. doi: 10.1128/jb.109.2.691-695.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Soprey P., Slade H. D. Chemical structure and immunological specificity of the streptococcal group e cell wall polysaccharide antigen. Infect Immun. 1971 May;3(5):653–658. doi: 10.1128/iai.3.5.653-658.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Soprey P., Slade H. D. Immunochemistry of the streptococcal group R cell wall polysaccharide antigen. Infect Immun. 1972 Jan;5(1):91–97. doi: 10.1128/iai.5.1.91-97.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. WYATT G. R. The purine and pyrimidine composition of deoxypentose nucleic acids. Biochem J. 1951 May;48(5):584–590. doi: 10.1042/bj0480584. [DOI] [PMC free article] [PubMed] [Google Scholar]

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