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. 1973 Jan;7(1):5–8. doi: 10.1128/iai.7.1.5-8.1973

Assay of Typhoid Vaccines with Salmonella typhosa-Salmonella typhimurium Hybrids

B B Diena 1,2, E M Johnson 1,2, L S Baron 1,2, R Wallace 1,2, L Greenberg 1,2
PMCID: PMC422630  PMID: 4120357

Abstract

Salmonella typhimurium hybrids expressing the S. typhosa antigens 9, d, and Vi were constructed by genetic crosses with an S. typhosa Hfr donor. The hybrids retained the same degree of mouse virulence as their S. typhimurium parent strain, the minimum lethal dose being less than 50 organisms when tested either in C57 black mice or Swiss white mice. Vaccination of the Swiss white mice with S. typhosa Ty2 vaccines prepared by acetone treatment, alcohol treatment, or heat-killing conferred significant protection against challenge by the hybrid strains but not against their S. typhimurium parent. Both the acetone-treated and alcohol-treated typhoid vaccines were markedly more protective than the heat-killed, phenol-preserved vaccine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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