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. 1973 Feb;7(2):178–189. doi: 10.1128/iai.7.2.178-189.1973

Properties of the Enterotoxic Component in Escherichia coli Enteropathogenic for Swine

Thomas M Jacks 1, Bo J Wu 1, Allen C Braemer 1, Donald E Bidlack 1
PMCID: PMC422657  PMID: 4633289

Abstract

The enterotoxic component in sterile syncase broth filtrates of Escherichia coli strains 340 (O9:K·:NM) and P307 (O8:K87,88a,b:H19) was studied. The enterotoxic activity in both strains was retained by an ultrafiltration membrane with a molecular weight retention of 100,000 (XM-100A) and eluted from a Sephadex G-200 column in the void volume. The enterotoxic activity in strain 340 was resistant to heating at 75 C for 30 min, but the activity in strain P307 was destroyed by heating at 60 C for 30 min. The P307 Sephadex G-200 column eluate possessing the enterotoxic activity, when desalted, contained 45.8% carbohydrate and 9.3% protein, and had an ED50 of 2.2 mg/rabbit ileal loop. Immunodiffusion studies showed that this material contained both endotoxin and acid-polysaccharide capsular material. The enterotoxic activity was acidlabile and was destroyed by Pronase, but was resistant to trypsin and eluted as a single peak in the void volume of a 4% agarose column. The enterotoxic component could not be separated from the endotoxin; in fact, the data indicated that the two components are closely associated and that the enterotoxic activity resides in material of a protein nature.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burrows W., Musteikis G. M. Cholera infection and toxin in the rabbit ileal loop. J Infect Dis. 1966 Apr;116(2):183–190. doi: 10.1093/infdis/116.2.183. [DOI] [PubMed] [Google Scholar]
  2. Dawson G., Clamp J. R. Investigations on the oligosaccharide units of an A myeloma globulin. Biochem J. 1968 Apr;107(3):341–352. doi: 10.1042/bj1070341. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Etkin S., Gorbach S. L. Studies on enterotoxin from Escherichia coli associated with acute diarrhea in man. J Lab Clin Med. 1971 Jul;78(1):81–87. [PubMed] [Google Scholar]
  4. Finkelstein R. A., Atthasampunna P., Chulasamaya M., Charunmethee P. Pathogenesis of experimental cholera: biologic ativities of purified procholeragen A. J Immunol. 1966 Mar;96(3):440–449. [PubMed] [Google Scholar]
  5. Finkelstein R. A., LoSpalluto J. J. Crystalline cholera toxin and toxoid. Science. 1972 Feb 4;175(4021):529–530. doi: 10.1126/science.175.4021.529. [DOI] [PubMed] [Google Scholar]
  6. Grady G. F., Keusch G. T. Pathogenesis of bacterial diarrheas. I. N Engl J Med. 1971 Oct 7;285(15):831–841. doi: 10.1056/NEJM197110072851505. [DOI] [PubMed] [Google Scholar]
  7. Gyles C. L., Barnum D. A. A heat-labile enterotoxin from strains of Eschericha coli enteropathogenic for pigs. J Infect Dis. 1969 Oct;120(4):419–426. doi: 10.1093/infdis/120.4.419. [DOI] [PubMed] [Google Scholar]
  8. HUMMEL B. C. A modified spectrophotometric determination of chymotrypsin, trypsin, and thrombin. Can J Biochem Physiol. 1959 Dec;37:1393–1399. [PubMed] [Google Scholar]
  9. Kohler E. M. Enterotoxic activity of filtrates of escherichia coli in young pigs. Am J Vet Res. 1968 Dec;29(12):2263–2274. [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Moon H. W., Whipp S. C., Engstrom G. W., Baetz A. L. Response of the rabbit ileal loop to cell-free products from Escherichia coli enteropathogenic for swine. J Infect Dis. 1970 Feb;121(2):182–187. doi: 10.1093/infdis/121.2.182. [DOI] [PubMed] [Google Scholar]
  12. Orskov I., Orskov F. The K antigens of Escherichia coli. Re-examination and re-evaluation of the nature of L antigens. Acta Pathol Microbiol Scand B Microbiol Immunol. 1970;78(5):593–604. [PubMed] [Google Scholar]
  13. SCHROHENLOHER R. E., KUNKEL H. G., TOMASI T. B. ACTIVITY OF DISSOCIATED AND REASSOCIATED 19S ANTI-GAMMA-GLOBULINS. J Exp Med. 1964 Dec 1;120:1215–1229. doi: 10.1084/jem.120.6.1215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. STELLWAGEN E., SCHACHMAN H. K. The dissociation and reconstitution of aldolase. Biochemistry. 1962 Nov;1:1056–1069. doi: 10.1021/bi00912a016. [DOI] [PubMed] [Google Scholar]
  15. Sack R. B., Gorbach S. L., Banwell J. G., Jacobs B., Chatterjee B. D., Mitra R. C. Enterotoxigenic Escherichia coli isolated from patients with severe cholera-like disease. J Infect Dis. 1971 Apr;123(4):378–385. doi: 10.1093/infdis/123.4.378. [DOI] [PubMed] [Google Scholar]
  16. Smith H. W., Gyles C. L. The effect of cell-free fluids prepared from cultures of human and animal enteropathogenic strains of Escherichia coli on ligated intestinal segments of rabbits and pigs. J Med Microbiol. 1970 Aug;3(3):403–409. doi: 10.1099/00222615-3-3-403. [DOI] [PubMed] [Google Scholar]
  17. Smith H. W., Gyles C. L. The relationship between two apparently different enterotoxins produced by enteropathogenic strains of Escherichia coli of porcine origin. J Med Microbiol. 1970 Aug;3(3):387–401. doi: 10.1099/00222615-3-3-387. [DOI] [PubMed] [Google Scholar]
  18. Smith H. W., Halls S. Studies on Escherichia coli enterotoxin. J Pathol Bacteriol. 1967 Apr;93(2):531–543. doi: 10.1002/path.1700930212. [DOI] [PubMed] [Google Scholar]
  19. Stirm S., Orskov F., Orskov I., Mansa B. Episome-carried surface antigen K88 of Escherichia coli. II. Isolation and chemical analysis. J Bacteriol. 1967 Feb;93(2):731–739. doi: 10.1128/jb.93.2.731-739.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Tarcsay L., Jann B., Jann K. Immunochemistry of the K antigens of Escherichia coli. The K87 antigen from Escherichia coli 08:K87(B?):H19. Eur J Biochem. 1971 Dec 10;23(3):505–514. doi: 10.1111/j.1432-1033.1971.tb01647.x. [DOI] [PubMed] [Google Scholar]
  21. Truszczyński M., Ciosek D. Antigenic properties of enterotoxins and water extracts obtained from Escherichia coli pathogenic for pigs. Res Vet Sci. 1972 May;13(3):205–211. [PubMed] [Google Scholar]
  22. Truszczyński M., Ciosek D. The influence of the contents of different K antigens and of physical factors on the toxicity of enterotoxins and water extracts of Escherichia coli. Res Vet Sci. 1972 May;13(3):212–218. [PubMed] [Google Scholar]

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